Detailed information NTG1097469

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACM0O0_RS14555
• Genome accession: NZ_CP180689
• Coordinates: 3120034..3121152 (+)
• Length: 372 a.a.
• NCBI ID: WP_005803129.1
• Uniprot ID: A0A009MM04
• Organism: Acinetobacter lactucae isolate nanjing
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3115034..3126152

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACM0O0_RS14545	-	3118157..3118849 (-)	693	WP_373357915.1	YggS family pyridoxal phosphate-dependent enzyme	-
ACM0O0_RS14550	pilT	3118976..3120013 (+)	1038	WP_081175033.1	type IV pilus twitching motility protein PilT	Machinery gene
ACM0O0_RS14555	pilU	3120034..3121152 (+)	1119	WP_005803129.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACM0O0_RS14560	fur	3121222..3121659 (-)	438	WP_002121758.1	ferric iron uptake transcriptional regulator	-
ACM0O0_RS14565	-	3121771..3122169 (+)	399	WP_002121740.1	outer membrane protein assembly factor BamE	-
ACM0O0_RS14570	-	3122234..3122548 (-)	315	WP_016144536.1	RnfH family protein	-
ACM0O0_RS14575	-	3122548..3123615 (-)	1068	WP_373357917.1	hypothetical protein	-
ACM0O0_RS14580	-	3123769..3124224 (-)	456	WP_002121698.1	bacteriohemerythrin	-
ACM0O0_RS14585	-	3124476..3125324 (-)	849	WP_416040612.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41903.09 Da
• Isoelectric Point: 6.6403

>NTDB_id=1097469 ACM0O0_RS14555 WP_005803129.1 3120034..3121152(+) (pilU) [Acinetobacter lactucae isolate nanjing]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEIIGQLLHSIMSEKQRKEFAETRECNFAIMNRDKSA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISYRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKSGQITYKDALKHADSPNDLRLTIKLAEEGPDQIAGTNQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=1097469 ACM0O0_RS14555 WP_005803129.1 3120034..3121152(+) (pilU) [Acinetobacter lactucae isolate nanjing]
ATGGATTTTAATGACCTACTCAACCTGATGGTTGAAAAAAAATCATCCGACCTCTTTATTACAGATGGCGTTGCGCCATC
GATGAAGATTAACGGGCAAATTGTTCCCATTTCAAAAAATAGTCTTTCAGGTGAAATTATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCTGAGACTCGTGAATGTAACTTTGCCATTATGAACCGAGATAAATCTGCC
CGTTTTCGTGTGAGTGCTTTTCAGCAACGTGACATGCCGGGCATGGTATTACGTCGAATTGAAACTAAAATTCCTTCAAT
TGATGACTTACAACTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGTATCATTATTTTTGTAGGCGCAACAG
GTACAGGTAAATCGACCTCATTGGCTTCAATGATTAGCTATCGGAACCATAACTCTAAAGGTCACATCATTACCATTGAA
GACCCTATCGAGTTTATTCATGAACACGCTGGCTGCATTATTACCCAACGTGAAGTTGGGATCGATACAGACTCATTCGA
AATTGCCTTAAAGAATACTTTGCGACAAGCACCCGATGTAATCTTGATTGGTGAGATTCGTTCTCGTGAAGTAATGGACT
ACGCAATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCCACAATGCACGCCAACAACGCTAACCAAGCACTTGATCGT
ATTATTCACTTCTTTGAAAGTGATCGTCACAGCCAGCTCTACATGGACCTATCACTTAACTTAAAGGCCATGATTGCACA
GCAACTTATTCCAACACCTGATGGTAATTCGCGCCGTGCAGCGATTGAGATTCTCATTAACTCACCATTAATTTCAGACT
ATATTCGTAAAGGCGAAATTCATGAAATTAAAGATTTAATGAAACGCTCACGTGAACTCGGTATGCAAACTTTTGACCAA
GCGCTGTTTGATCTTTATAAGTCAGGCCAAATTACCTACAAAGATGCATTAAAACATGCCGATTCACCAAACGATTTACG
TTTAACAATTAAGCTTGCCGAAGAAGGTCCTGATCAAATTGCAGGCACAAATCAGCATTTAACCTTCGACAGACAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (32-270)

3D structure

• AlphaFold DB: A0A009MM04

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.397	100	0.766
pilU	Pseudomonas stutzeri DSM 10701	68.464	99.731	0.683
pilU	Vibrio cholerae strain A1552	54.324	99.462	0.54
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.934	97.849	0.401
pilT	Legionella pneumophila strain Lp02	39.941	90.86	0.363
pilT	Legionella pneumophila strain ERS1305867	39.941	90.86	0.363