Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACM2ZB_RS09700 Genome accession   NZ_CP180589
Coordinates   2082531..2083568 (-) Length   345 a.a.
NCBI ID   WP_004147099.1    Uniprot ID   A0AAP7J2J0
Organism   Stenotrophomonas maltophilia strain ZSL2493     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2077531..2088568
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACM2ZB_RS09675 (ACM2ZB_09675) - 2077873..2078772 (+) 900 WP_416058326.1 LysR substrate-binding domain-containing protein -
  ACM2ZB_RS09680 (ACM2ZB_09680) - 2078805..2079788 (-) 984 WP_416058327.1 aldo/keto reductase -
  ACM2ZB_RS09685 (ACM2ZB_09685) - 2079880..2080452 (-) 573 WP_416058328.1 hypothetical protein -
  ACM2ZB_RS09690 (ACM2ZB_09690) - 2080566..2081207 (-) 642 WP_416058329.1 PIN domain-containing protein -
  ACM2ZB_RS09695 (ACM2ZB_09695) pilU 2081283..2082434 (-) 1152 WP_313435065.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACM2ZB_RS09700 (ACM2ZB_09700) pilT 2082531..2083568 (-) 1038 WP_004147099.1 type IV pilus twitching motility protein PilT Machinery gene
  ACM2ZB_RS09705 (ACM2ZB_09705) - 2083656..2084333 (+) 678 WP_416058330.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACM2ZB_RS09710 (ACM2ZB_09710) proC 2084350..2085171 (+) 822 WP_416058331.1 pyrroline-5-carboxylate reductase -
  ACM2ZB_RS09715 (ACM2ZB_09715) - 2086017..2086889 (-) 873 WP_416058332.1 LysR family transcriptional regulator -
  ACM2ZB_RS09720 (ACM2ZB_09720) - 2086986..2087588 (+) 603 WP_416058333.1 short chain dehydrogenase -
  ACM2ZB_RS09725 (ACM2ZB_09725) soxR 2087635..2088066 (-) 432 WP_416058537.1 redox-sensitive transcriptional activator SoxR -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38658.41 Da        Isoelectric Point: 6.6654

>NTDB_id=1096854 ACM2ZB_RS09700 WP_004147099.1 2082531..2083568(-) (pilT) [Stenotrophomonas maltophilia strain ZSL2493]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLACPPLFREVIQQPQGLILVTGPTGSGKSTTLAAMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQQYGMMTLDQHL
QDLVKRSLITRNQAREYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1096854 ACM2ZB_RS09700 WP_004147099.1 2082531..2083568(-) (pilT) [Stenotrophomonas maltophilia strain ZSL2493]
ATGGATATCGCCGAACTGTTGGCGTTTTCCGTAAAGAACAAAGCGTCCGACCTGCACCTGTCTGCAGGCCTGCCGCCGAT
GATCCGCGTGGACGGTGACGTCCGCCGCATCAACATCCCGGCCCTGGACCACAAGCAGGTCCACGCGCTGGTGTACGACA
TCATGTCCGACAAGCAGCGGCGCGATTACGAGGAATTCCTCGAAGTCGACTTCTCCTTCGAGATTCCGTCGCTGGCCCGC
TTCCGTGTCAATGCCTTCAACCAGAACCGTGGCGCCGGTGCGGTGTTCCGTACCATTCCGTCCGAAGTGCTGACCCTGGA
GGACCTGGCCTGCCCGCCGCTGTTCCGCGAGGTCATCCAGCAGCCGCAGGGCCTGATCCTGGTGACCGGCCCGACCGGTT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCGACTACATCAACAAGAACGAATACGGCCACATCCTCACCGTCGAGGAT
CCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTGCACCGCGACACGCACGGCTTCAACGA
AGCACTGCGCTCGGCACTGCGTGAGGATCCGGACATCATCCTGGTGGGCGAACTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCTGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCAGCTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAATCGCTGCGCGCGGTCATTTCGCAGGC
GCTGCTGAAGAAGGTCGGCGGCGGCCGTACCGCTGCGTGGGAAATCATGGTCGGCACCCCGGCCATCCGCAACCTGATCC
GCGAAGACAAGGTGGCGCAGATGTACTCGGCCATCCAGACCGGCCAGCAGTACGGAATGATGACCCTTGACCAGCACCTG
CAGGACCTGGTCAAGCGCAGCCTGATCACCCGCAACCAGGCGCGCGAGTACGCCAAGGACAAGCGTCTGTTCGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.651

99.71

0.794
  pilT Acinetobacter baumannii D1279779

79.651

99.71

0.794
  pilT Acinetobacter baumannii strain A118

79.651

99.71

0.794
  pilT Pseudomonas stutzeri DSM 10701

78.488

99.71

0.783
  pilT Acinetobacter baylyi ADP1

77.907

99.71

0.777
  pilT Pseudomonas aeruginosa PAK

79.056

98.261

0.777
  pilT Legionella pneumophila strain Lp02

75

99.71

0.748
  pilT Legionella pneumophila strain ERS1305867

75

99.71

0.748
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.821

95.362

0.675
  pilT Vibrio cholerae strain A1552

70.821

95.362

0.675
  pilT Neisseria meningitidis 8013

65.689

98.841

0.649
  pilT Neisseria gonorrhoeae MS11

65.396

98.841

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.565

100

0.496
  pilU Vibrio cholerae strain A1552

39.943

100

0.403
  pilU Pseudomonas stutzeri DSM 10701

40.413

98.261

0.397
  pilU Acinetobacter baylyi ADP1

39.706

98.551

0.391

Multiple sequence alignment