Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACM2ZB_RS09695 Genome accession   NZ_CP180589
Coordinates   2081283..2082434 (-) Length   383 a.a.
NCBI ID   WP_313435065.1    Uniprot ID   -
Organism   Stenotrophomonas maltophilia strain ZSL2493     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2076283..2087434
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACM2ZB_RS09665 (ACM2ZB_09665) - 2076386..2077093 (-) 708 WP_200733422.1 YitT family protein -
  ACM2ZB_RS09670 (ACM2ZB_09670) - 2077314..2077526 (-) 213 WP_416058325.1 DUF4287 domain-containing protein -
  ACM2ZB_RS09675 (ACM2ZB_09675) - 2077873..2078772 (+) 900 WP_416058326.1 LysR substrate-binding domain-containing protein -
  ACM2ZB_RS09680 (ACM2ZB_09680) - 2078805..2079788 (-) 984 WP_416058327.1 aldo/keto reductase -
  ACM2ZB_RS09685 (ACM2ZB_09685) - 2079880..2080452 (-) 573 WP_416058328.1 hypothetical protein -
  ACM2ZB_RS09690 (ACM2ZB_09690) - 2080566..2081207 (-) 642 WP_416058329.1 PIN domain-containing protein -
  ACM2ZB_RS09695 (ACM2ZB_09695) pilU 2081283..2082434 (-) 1152 WP_313435065.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACM2ZB_RS09700 (ACM2ZB_09700) pilT 2082531..2083568 (-) 1038 WP_004147099.1 type IV pilus twitching motility protein PilT Machinery gene
  ACM2ZB_RS09705 (ACM2ZB_09705) - 2083656..2084333 (+) 678 WP_416058330.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACM2ZB_RS09710 (ACM2ZB_09710) proC 2084350..2085171 (+) 822 WP_416058331.1 pyrroline-5-carboxylate reductase -
  ACM2ZB_RS09715 (ACM2ZB_09715) - 2086017..2086889 (-) 873 WP_416058332.1 LysR family transcriptional regulator -

Sequence

Protein


Download         Length: 383 a.a.        Molecular weight: 42580.92 Da        Isoelectric Point: 6.7684

>NTDB_id=1096853 ACM2ZB_RS09695 WP_313435065.1 2081283..2082434(-) (pilU) [Stenotrophomonas maltophilia strain ZSL2493]
MNSTASTASTIDFTSFLKLMAHQRASDLFITAGMPPAIKVNGKISPITQTPLTPQQSRDLVLNVMTPAQREEFEKTHECN
FAIGLAGVGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIGYRNQN
STGHIITIEDPIEFVHKHEGCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHA
NNANQAMDRIINFFPEDRRNQLLMDLSLNLKGVVAQQLVPSPDGRSRKVAMEILLGTPLVQDYIRDGEIHKLKELMKESV
QLGMKTFDQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDARTLSQGLDGVEISEVR

Nucleotide


Download         Length: 1152 bp        

>NTDB_id=1096853 ACM2ZB_RS09695 WP_313435065.1 2081283..2082434(-) (pilU) [Stenotrophomonas maltophilia strain ZSL2493]
ATGAACAGCACCGCCAGCACTGCCAGCACCATCGATTTCACCTCCTTCCTCAAGCTGATGGCGCACCAGCGCGCCTCGGA
CCTGTTCATCACCGCCGGCATGCCGCCGGCGATCAAGGTCAACGGCAAGATCTCGCCGATCACGCAGACGCCGCTCACCC
CGCAACAGAGCCGCGACCTGGTCCTCAACGTGATGACCCCGGCCCAGCGCGAGGAATTCGAGAAGACCCACGAGTGCAAC
TTCGCCATCGGCCTGGCCGGTGTCGGCCGTTTCCGCGTGAGCTGCTTCTACCAGCGCAACCAGGTCGGCATGGTCCTGCG
TCGCATCGAAACGCGCATCCCGACGGTGGAAGAACTGAGCCTGCCGCCTATCATCAAGACGCTGGCGATGACCAAGCGCG
GCATCATCCTGTTCGTGGGCGCCACCGGTACCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAAT
TCGACCGGCCACATCATCACCATCGAAGATCCGATCGAGTTCGTGCACAAGCATGAAGGCTGCATCATCACCCAGCGCGA
AGTCGGCATCGACACCGACAGCTGGGAAGCGGCGCTGAAGAACACCCTGCGCCAGGCACCGGACGTGATCATGATCGGCG
AGGTGCGTACCCGCGAGGGCATGGACCACGCCATCGCCTTCGCAGAAACCGGCCACCTGGTGCTGTGCACCCTGCATGCC
AACAACGCCAACCAGGCGATGGACCGCATCATCAACTTCTTCCCCGAAGACCGCCGCAACCAGCTGCTGATGGATCTGTC
GCTGAACCTCAAGGGCGTGGTCGCCCAGCAGCTGGTGCCGTCGCCCGATGGCCGCTCGCGCAAGGTGGCGATGGAAATCC
TGCTGGGCACGCCGCTGGTGCAGGACTACATCCGCGACGGCGAGATCCACAAGCTGAAGGAACTGATGAAGGAGTCGGTC
CAGCTGGGCATGAAGACCTTCGACCAGAGCCTGTTCGAACTGTACCAGGCCGGCGAGATCAGCTACGAGGACGCGCTGCG
CTACGCCGATTCGCAGAACGAAGTGCGCCTGCGCATCAAGCTCAGCCAGGGCGGTGATGCGCGCACCCTGTCGCAGGGCC
TGGACGGCGTGGAGATTTCCGAGGTTCGTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(125-280)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.12

95.822

0.653
  pilU Acinetobacter baylyi ADP1

64.146

93.211

0.598
  pilU Vibrio cholerae strain A1552

53.371

92.95

0.496
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.577

93.211

0.397
  pilT Acinetobacter nosocomialis M2

40.116

89.817

0.36
  pilT Acinetobacter baumannii D1279779

40.116

89.817

0.36
  pilT Acinetobacter baumannii strain A118

40.116

89.817

0.36

Multiple sequence alignment