Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACMXYW_RS01395 Genome accession   NZ_CP180555
Coordinates   308225..309259 (+) Length   344 a.a.
NCBI ID   WP_415904958.1    Uniprot ID   -
Organism   Neptuniibacter sp. QD48_55     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 303225..314259
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMXYW_RS01365 (ACMXYW_01365) - 303430..303879 (-) 450 WP_415904955.1 DUF4426 domain-containing protein -
  ACMXYW_RS01370 (ACMXYW_01370) metW 303892..304482 (-) 591 WP_415893171.1 methionine biosynthesis protein MetW -
  ACMXYW_RS01375 (ACMXYW_01375) metX 304482..305651 (-) 1170 WP_415904956.1 homoserine O-succinyltransferase MetX -
  ACMXYW_RS01380 (ACMXYW_01380) - 305948..306493 (-) 546 WP_415893173.1 YggT family protein -
  ACMXYW_RS01385 (ACMXYW_01385) proC 306592..307419 (-) 828 WP_415904957.1 pyrroline-5-carboxylate reductase -
  ACMXYW_RS01390 (ACMXYW_01390) - 307438..308133 (-) 696 WP_415899878.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACMXYW_RS01395 (ACMXYW_01395) pilT 308225..309259 (+) 1035 WP_415904958.1 type IV pilus twitching motility protein PilT Machinery gene
  ACMXYW_RS01400 (ACMXYW_01400) pilU 309282..310448 (+) 1167 WP_415904959.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACMXYW_RS01405 (ACMXYW_01405) - 310524..311798 (-) 1275 WP_415904960.1 dihydroorotase -
  ACMXYW_RS01410 (ACMXYW_01410) - 311804..312808 (-) 1005 WP_415899881.1 aspartate carbamoyltransferase catalytic subunit -
  ACMXYW_RS01415 (ACMXYW_01415) pyrR 312818..313333 (-) 516 WP_415904961.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  ACMXYW_RS01420 (ACMXYW_01420) ruvX 313314..313790 (-) 477 WP_415899883.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38256.87 Da        Isoelectric Point: 6.5797

>NTDB_id=1096530 ACMXYW_RS01395 WP_415904958.1 308225..309259(+) (pilT) [Neptuniibacter sp. QD48_55]
MDITELLSFTVQQGASDLHISAGMPPIIRVDGDVRRIKLPSLDHKQVHTLIYDIMNDKQRKEYEDVLETDFSFEVPGLAR
FRVNAFNQNRGAGAVFRTIPSKVLTLDDLDMGKVFQDLSEKPRGLVLVTGPTGSGKSTTLAAMIDYINDTRADHILTIED
PIEFVHESKKCLVNQREVHRDTHSFSNALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAAEKDMVRSMLSESLMGVISQTLLKKPKGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGASFGMKTLDQSL
QELLHKGLITRETARERAANPQQF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1096530 ACMXYW_RS01395 WP_415904958.1 308225..309259(+) (pilT) [Neptuniibacter sp. QD48_55]
ATGGATATTACCGAACTTTTATCATTTACCGTACAACAAGGTGCATCAGATTTGCATATATCAGCAGGGATGCCACCTAT
TATTCGTGTTGACGGTGATGTGCGTCGCATAAAACTGCCTTCTTTGGACCATAAACAGGTTCATACTCTTATCTACGACA
TTATGAATGATAAGCAACGTAAAGAGTATGAAGATGTACTCGAGACAGACTTTTCGTTCGAGGTTCCTGGTTTAGCGCGT
TTTCGTGTCAATGCATTTAACCAGAACCGAGGTGCAGGTGCTGTTTTCCGTACGATCCCAAGCAAGGTGTTGACCCTAGA
TGATCTTGATATGGGTAAGGTTTTTCAGGACTTATCTGAAAAACCGCGTGGTTTAGTGCTTGTGACGGGGCCAACGGGTT
CAGGTAAAAGTACCACCTTGGCCGCAATGATCGATTACATTAATGATACTCGCGCAGATCATATTTTGACGATCGAAGAC
CCAATTGAATTTGTGCACGAAAGTAAGAAGTGCTTGGTTAACCAGCGTGAAGTGCATAGGGATACACATAGCTTTTCGAA
TGCACTTCGTTCGGCACTACGTGAAGATCCGGATATCATTCTTGTTGGTGAGATGCGAGACTTGGAAACTATCCGCCTCG
CGTTGACTGCAGCTGAAACCGGCCACTTAGTATTTGGTACCCTGCACACGACCTCAGCTGCGAAAACAATTGACCGTGTA
ATTGACGTATTTCCAGCGGCTGAGAAAGATATGGTGCGATCCATGCTTTCAGAGTCGTTAATGGGTGTTATATCGCAAAC
GCTTTTGAAGAAGCCAAAAGGAGGACGTGTTGCGGCTCACGAGATTATGATTGGTACGCCGGCTATTCGTAATCTTATCC
GAGAAGATAAAGTGGCTCAGATGTATTCGGCTATTCAAACCGGTGCGTCATTTGGTATGAAAACGTTGGATCAATCTCTA
CAAGAATTGCTGCATAAAGGATTGATTACACGCGAAACGGCGCGTGAGCGTGCAGCCAACCCACAACAGTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

82.849

100

0.828
  pilT Pseudomonas stutzeri DSM 10701

82.267

100

0.823
  pilT Acinetobacter nosocomialis M2

80.523

100

0.805
  pilT Acinetobacter baumannii D1279779

80.523

100

0.805
  pilT Acinetobacter baumannii strain A118

80.523

100

0.805
  pilT Acinetobacter baylyi ADP1

80.233

100

0.802
  pilT Legionella pneumophila strain Lp02

72.965

100

0.73
  pilT Legionella pneumophila strain ERS1305867

72.965

100

0.73
  pilT Neisseria meningitidis 8013

67.055

99.709

0.669
  pilT Neisseria gonorrhoeae MS11

66.764

99.709

0.666
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.997

95.64

0.66
  pilT Vibrio cholerae strain A1552

68.997

95.64

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

100

0.512
  pilU Pseudomonas stutzeri DSM 10701

41.791

97.384

0.407
  pilU Vibrio cholerae strain A1552

41.846

94.477

0.395
  pilU Acinetobacter baylyi ADP1

39.017

100

0.392

Multiple sequence alignment