Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACMXYW_RS01400 Genome accession   NZ_CP180555
Coordinates   309282..310448 (+) Length   388 a.a.
NCBI ID   WP_415904959.1    Uniprot ID   -
Organism   Neptuniibacter sp. QD48_55     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 304282..315448
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMXYW_RS01375 (ACMXYW_01375) metX 304482..305651 (-) 1170 WP_415904956.1 homoserine O-succinyltransferase MetX -
  ACMXYW_RS01380 (ACMXYW_01380) - 305948..306493 (-) 546 WP_415893173.1 YggT family protein -
  ACMXYW_RS01385 (ACMXYW_01385) proC 306592..307419 (-) 828 WP_415904957.1 pyrroline-5-carboxylate reductase -
  ACMXYW_RS01390 (ACMXYW_01390) - 307438..308133 (-) 696 WP_415899878.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACMXYW_RS01395 (ACMXYW_01395) pilT 308225..309259 (+) 1035 WP_415904958.1 type IV pilus twitching motility protein PilT Machinery gene
  ACMXYW_RS01400 (ACMXYW_01400) pilU 309282..310448 (+) 1167 WP_415904959.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACMXYW_RS01405 (ACMXYW_01405) - 310524..311798 (-) 1275 WP_415904960.1 dihydroorotase -
  ACMXYW_RS01410 (ACMXYW_01410) - 311804..312808 (-) 1005 WP_415899881.1 aspartate carbamoyltransferase catalytic subunit -
  ACMXYW_RS01415 (ACMXYW_01415) pyrR 312818..313333 (-) 516 WP_415904961.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  ACMXYW_RS01420 (ACMXYW_01420) ruvX 313314..313790 (-) 477 WP_415899883.1 Holliday junction resolvase RuvX -
  ACMXYW_RS01425 (ACMXYW_01425) - 313842..314414 (-) 573 WP_415899884.1 YqgE/AlgH family protein -
  ACMXYW_RS01430 (ACMXYW_01430) - 314452..315384 (-) 933 WP_415904962.1 energy transducer TonB -

Sequence

Protein


Download         Length: 388 a.a.        Molecular weight: 43411.48 Da        Isoelectric Point: 6.0156

>NTDB_id=1096531 ACMXYW_RS01400 WP_415904959.1 309282..310448(+) (pilU) [Neptuniibacter sp. QD48_55]
MDFTQLLKVMVDRDASDLFVTAGARPTIKVDGTLKPLTKDTLKPSQARALVYSTMNDKQLAEFEGCHECNFAISAPGLGR
FRVSAFFQRNSAGMVLRKINNYIPSLEELNLPPILRDLSMTKRGLILFVGGTSTGKSTSLASMIDYRNTNHRGHIITIED
PIEYVHEHKQSIITQREVGIDTDSFDVALKNTLRQAPDVILMGEIRTRETMQHGIVFAETGHLTLATLHANNANQALDRV
ISFFPPEHHDQLWMDLSLNLKAIIAQQLLPTKDGKGRRAAIEVLINTPLIQDLIRKGEVHEIKDVVKKSTNLGMQTFDQS
LFNLYKDGVITYDVALAHADSPNDLRLMIKLSADTNPELAPEEDNQSFHLQDEEDYLHNEGDANVKGM

Nucleotide


Download         Length: 1167 bp        

>NTDB_id=1096531 ACMXYW_RS01400 WP_415904959.1 309282..310448(+) (pilU) [Neptuniibacter sp. QD48_55]
GTGGATTTTACGCAACTACTAAAAGTGATGGTAGATAGGGATGCCTCTGACCTTTTCGTAACGGCCGGTGCAAGGCCAAC
GATTAAAGTTGATGGTACTTTGAAACCGCTAACCAAAGACACTCTGAAGCCTTCACAGGCTAGGGCTCTTGTTTACAGCA
CAATGAATGATAAACAGTTGGCAGAGTTTGAGGGTTGTCATGAGTGTAACTTTGCGATTAGTGCACCAGGTTTGGGCCGT
TTTCGTGTGTCAGCATTTTTCCAGCGTAACTCAGCGGGTATGGTGTTAAGGAAAATTAATAACTATATTCCAAGTTTAGA
AGAGTTGAATTTGCCACCAATCCTGCGTGATTTGTCTATGACAAAGCGTGGCTTGATCTTATTTGTTGGTGGTACTTCTA
CAGGTAAATCTACCTCTTTAGCATCGATGATTGATTACCGAAATACCAATCATCGCGGTCATATTATTACCATCGAAGAT
CCGATTGAATACGTTCACGAACATAAGCAAAGTATCATCACTCAACGTGAAGTAGGTATCGATACAGACTCGTTTGATGT
GGCATTAAAAAATACGCTGCGTCAGGCACCAGATGTAATTTTGATGGGTGAGATCCGTACTAGGGAGACGATGCAACATG
GTATTGTCTTTGCTGAAACAGGCCACTTAACTTTGGCAACGTTACATGCCAACAATGCGAACCAAGCATTAGACCGTGTA
ATTAGTTTCTTCCCACCTGAACATCATGATCAGTTGTGGATGGATCTTTCGCTGAACTTGAAGGCGATTATTGCGCAACA
ACTTTTACCTACTAAAGATGGTAAGGGGCGTCGTGCAGCGATCGAAGTATTGATTAACACACCATTGATTCAGGATTTGA
TCCGTAAAGGTGAAGTTCATGAAATTAAAGATGTGGTTAAGAAATCGACCAATTTAGGTATGCAGACGTTCGACCAATCT
TTGTTTAATCTCTACAAAGATGGGGTGATTACTTACGATGTAGCACTAGCTCATGCTGATTCGCCAAACGATCTTCGTCT
CATGATTAAGTTGAGTGCTGATACGAATCCTGAATTGGCTCCAGAGGAAGATAATCAATCTTTTCATCTCCAGGATGAAG
AGGATTACCTACATAACGAAGGCGATGCTAACGTTAAGGGGATGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.877

92.268

0.626
  pilU Acinetobacter baylyi ADP1

60.44

93.814

0.567
  pilU Vibrio cholerae strain A1552

55.143

90.206

0.497
  pilT Acinetobacter baylyi ADP1

44.737

88.144

0.394
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.058

88.918

0.392
  pilT Acinetobacter baumannii D1279779

43.86

88.144

0.387
  pilT Acinetobacter baumannii strain A118

43.86

88.144

0.387
  pilT Acinetobacter nosocomialis M2

43.86

88.144

0.387
  pilT Pseudomonas stutzeri DSM 10701

42.609

88.918

0.379
  pilT Pseudomonas aeruginosa PAK

42.609

88.918

0.379

Multiple sequence alignment