Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACMXYP_RS01365 Genome accession   NZ_CP180546
Coordinates   315559..316593 (+) Length   344 a.a.
NCBI ID   WP_415883222.1    Uniprot ID   -
Organism   Neptuniibacter sp. QD34_54     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 310559..321593
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMXYP_RS01335 (ACMXYP_01335) - 310764..311213 (-) 450 WP_415883216.1 DUF4426 domain-containing protein -
  ACMXYP_RS01340 (ACMXYP_01340) metW 311226..311816 (-) 591 WP_415883217.1 methionine biosynthesis protein MetW -
  ACMXYP_RS01345 (ACMXYP_01345) metX 311816..312985 (-) 1170 WP_415883218.1 homoserine O-succinyltransferase MetX -
  ACMXYP_RS01350 (ACMXYP_01350) - 313282..313827 (-) 546 WP_415883219.1 YggT family protein -
  ACMXYP_RS01355 (ACMXYP_01355) proC 313926..314753 (-) 828 WP_415883220.1 pyrroline-5-carboxylate reductase -
  ACMXYP_RS01360 (ACMXYP_01360) - 314772..315467 (-) 696 WP_415883221.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACMXYP_RS01365 (ACMXYP_01365) pilT 315559..316593 (+) 1035 WP_415883222.1 type IV pilus twitching motility protein PilT Machinery gene
  ACMXYP_RS01370 (ACMXYP_01370) pilU 316616..317782 (+) 1167 WP_415883223.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACMXYP_RS01375 (ACMXYP_01375) - 317859..319133 (-) 1275 WP_415883224.1 dihydroorotase -
  ACMXYP_RS01380 (ACMXYP_01380) - 319139..320143 (-) 1005 WP_415883225.1 aspartate carbamoyltransferase catalytic subunit -
  ACMXYP_RS01385 (ACMXYP_01385) pyrR 320153..320668 (-) 516 WP_415883226.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  ACMXYP_RS01390 (ACMXYP_01390) ruvX 320649..321125 (-) 477 WP_415883227.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38205.84 Da        Isoelectric Point: 6.5074

>NTDB_id=1096392 ACMXYP_RS01365 WP_415883222.1 315559..316593(+) (pilT) [Neptuniibacter sp. QD34_54]
MDITELLSFTVQQGASDLHISAGMPPIIRVDGDVRRIKLPSLDHKQVHTLVYDIMNDKQRKEYEDVLETDFSFEVPGLAR
FRVNAFNQNRGAGAVFRTIPSKVLTLDDLDMGKVFQDLSEKPRGLVLVTGPTGSGKSTTLAAMIDYINDTRADHILTIED
PIEFVHESKKCLVNQREVHRDTHSFANALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAAEKDMVRSMLSESLMGVISQTLLKKPKGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGAAFGMKTLDQSL
QELMQKGLISRETARERAANPQQF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1096392 ACMXYP_RS01365 WP_415883222.1 315559..316593(+) (pilT) [Neptuniibacter sp. QD34_54]
ATGGATATTACCGAACTTTTATCATTTACCGTACAACAAGGCGCATCTGACTTGCATATCTCTGCAGGGATGCCCCCTAT
TATTCGTGTTGACGGTGATGTGCGTCGCATTAAACTGCCTTCATTGGATCATAAGCAGGTCCACACACTCGTATACGACA
TTATGAACGATAAACAACGTAAAGAGTACGAAGATGTGCTCGAAACGGATTTTTCGTTCGAAGTGCCTGGTTTAGCGCGT
TTTCGTGTCAATGCATTTAACCAGAACCGTGGTGCAGGTGCTGTTTTTCGTACGATTCCGAGCAAGGTGTTGACCTTAGA
TGATCTTGATATGGGTAAGGTTTTTCAGGACTTGTCTGAAAAACCGCGTGGTTTAGTGCTTGTGACCGGGCCAACGGGTT
CCGGTAAAAGTACCACCTTGGCGGCAATGATCGATTACATCAATGATACTCGTGCAGATCATATTTTGACGATCGAAGAT
CCAATCGAATTCGTGCACGAAAGTAAAAAATGCTTAGTAAACCAACGAGAAGTGCATCGAGATACCCATAGCTTCGCTAA
CGCACTTCGTTCGGCGCTTCGTGAAGACCCAGATATTATCCTTGTAGGTGAGATGCGAGACTTGGAAACTATCCGTCTTG
CACTTACGGCTGCTGAAACAGGTCACTTAGTGTTTGGTACCTTGCATACCACCTCAGCAGCCAAAACAATCGACCGTGTG
ATTGACGTATTCCCAGCGGCTGAGAAAGATATGGTGCGATCCATGCTTTCAGAATCACTGATGGGCGTTATATCGCAAAC
ACTTTTGAAAAAGCCGAAAGGCGGACGTGTTGCAGCTCACGAAATTATGATCGGTACACCAGCTATTCGTAACCTTATTC
GTGAGGATAAAGTGGCGCAGATGTATTCTGCGATCCAGACGGGTGCTGCATTTGGTATGAAAACCTTGGATCAATCTCTA
CAAGAGTTGATGCAGAAAGGACTAATCTCGCGCGAGACAGCGCGTGAACGAGCAGCAAACCCACAACAATTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

82.267

100

0.823
  pilT Pseudomonas stutzeri DSM 10701

81.395

100

0.814
  pilT Acinetobacter nosocomialis M2

81.105

100

0.811
  pilT Acinetobacter baumannii D1279779

81.105

100

0.811
  pilT Acinetobacter baumannii strain A118

81.105

100

0.811
  pilT Acinetobacter baylyi ADP1

79.651

100

0.797
  pilT Legionella pneumophila strain Lp02

73.256

100

0.733
  pilT Legionella pneumophila strain ERS1305867

73.256

100

0.733
  pilT Neisseria meningitidis 8013

67.93

99.709

0.677
  pilT Neisseria gonorrhoeae MS11

67.638

99.709

0.674
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.359

97.965

0.66
  pilT Vibrio cholerae strain A1552

67.359

97.965

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

100

0.509
  pilU Pseudomonas stutzeri DSM 10701

41.791

97.384

0.407
  pilU Vibrio cholerae strain A1552

41.493

97.384

0.404
  pilU Acinetobacter baylyi ADP1

39.017

100

0.392

Multiple sequence alignment