Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACMXYP_RS01370 Genome accession   NZ_CP180546
Coordinates   316616..317782 (+) Length   388 a.a.
NCBI ID   WP_415883223.1    Uniprot ID   -
Organism   Neptuniibacter sp. QD34_54     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 311616..322782
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMXYP_RS01345 (ACMXYP_01345) metX 311816..312985 (-) 1170 WP_415883218.1 homoserine O-succinyltransferase MetX -
  ACMXYP_RS01350 (ACMXYP_01350) - 313282..313827 (-) 546 WP_415883219.1 YggT family protein -
  ACMXYP_RS01355 (ACMXYP_01355) proC 313926..314753 (-) 828 WP_415883220.1 pyrroline-5-carboxylate reductase -
  ACMXYP_RS01360 (ACMXYP_01360) - 314772..315467 (-) 696 WP_415883221.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACMXYP_RS01365 (ACMXYP_01365) pilT 315559..316593 (+) 1035 WP_415883222.1 type IV pilus twitching motility protein PilT Machinery gene
  ACMXYP_RS01370 (ACMXYP_01370) pilU 316616..317782 (+) 1167 WP_415883223.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACMXYP_RS01375 (ACMXYP_01375) - 317859..319133 (-) 1275 WP_415883224.1 dihydroorotase -
  ACMXYP_RS01380 (ACMXYP_01380) - 319139..320143 (-) 1005 WP_415883225.1 aspartate carbamoyltransferase catalytic subunit -
  ACMXYP_RS01385 (ACMXYP_01385) pyrR 320153..320668 (-) 516 WP_415883226.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  ACMXYP_RS01390 (ACMXYP_01390) ruvX 320649..321125 (-) 477 WP_415883227.1 Holliday junction resolvase RuvX -
  ACMXYP_RS01395 (ACMXYP_01395) - 321180..321752 (-) 573 WP_415883228.1 YqgE/AlgH family protein -
  ACMXYP_RS01400 (ACMXYP_01400) - 321789..322721 (-) 933 WP_415883229.1 TonB family protein -

Sequence

Protein


Download         Length: 388 a.a.        Molecular weight: 43384.43 Da        Isoelectric Point: 6.0154

>NTDB_id=1096393 ACMXYP_RS01370 WP_415883223.1 316616..317782(+) (pilU) [Neptuniibacter sp. QD34_54]
MDFTQLLKVMVDRDASDLFVTAGARPTIKVDGTLKPLTKDTLKPSQARALVYSTMNDKQLAEFEGSHECNFAISAPGLGR
FRVSAFFQRNSAGMVLRKINNYIPSLEELNLPPILRDLSMTKRGLILFVGGTSTGKSTSLASMIDYRNTNHRGHIITIED
PIEYIHEHKQSIITQREVGIDTDSFDVALKNTLRQAPDVILMGEIRTRETMQHGIVFAETGHLTLATLHANNANQALDRV
ISFFPPEHHDQLWMDLSLNLKAIIAQQLLPTKDGKGRRAAIEVLINTPLIQDLIRKGEVHEIKDVVKKSTNLGMQTFDQS
LFTLYKDGVITYDVALAHADSPNDLRLMIKLSADTNPELAVEDDNQSFHLQDEEDYLHNEGDANVKGM

Nucleotide


Download         Length: 1167 bp        

>NTDB_id=1096393 ACMXYP_RS01370 WP_415883223.1 316616..317782(+) (pilU) [Neptuniibacter sp. QD34_54]
GTGGACTTTACGCAGCTACTAAAGGTTATGGTAGATAGGGATGCATCTGACCTTTTTGTTACGGCCGGTGCAAGGCCAAC
GATTAAAGTTGACGGTACTTTGAAGCCGTTAACAAAAGACACCCTCAAGCCCTCTCAGGCGCGGGCACTTGTTTACAGTA
CCATGAATGATAAGCAGCTTGCTGAGTTTGAAGGCAGTCATGAGTGTAACTTTGCGATTAGTGCGCCGGGTCTAGGTCGT
TTCCGTGTTTCTGCATTCTTTCAGCGTAACTCAGCAGGGATGGTATTAAGGAAGATTAATAACTATATCCCGAGTTTAGA
AGAGCTCAATTTGCCACCTATTCTCCGTGATTTGTCGATGACTAAACGAGGGTTGATCCTGTTTGTAGGTGGTACCTCTA
CAGGTAAATCGACTTCCTTGGCATCGATGATTGATTACCGTAATACTAATCACCGTGGTCATATCATTACCATCGAAGAC
CCGATTGAATATATCCATGAGCACAAGCAAAGTATTATCACTCAGCGTGAAGTGGGTATCGATACAGACTCGTTTGATGT
GGCATTAAAGAATACGCTGCGCCAGGCGCCCGATGTGATTTTGATGGGTGAGATCCGTACTCGAGAAACGATGCAGCACG
GTATCGTATTTGCCGAAACGGGCCACTTAACTTTGGCAACATTGCACGCTAACAATGCTAACCAAGCATTGGATCGTGTT
ATCAGTTTCTTCCCGCCGGAACACCATGATCAGTTGTGGATGGATCTGTCGCTGAACTTGAAAGCGATCATAGCCCAGCA
ACTTTTGCCGACGAAAGATGGTAAAGGTCGTCGTGCAGCGATAGAGGTATTGATTAATACGCCATTGATTCAGGACCTTA
TCCGCAAGGGCGAAGTTCATGAGATCAAAGATGTCGTTAAGAAATCGACTAACTTAGGGATGCAGACGTTCGATCAGTCC
CTGTTTACTCTCTATAAAGATGGTGTGATTACTTACGATGTAGCGTTGGCTCATGCGGATTCTCCAAACGATCTCCGTCT
CATGATTAAGTTGAGTGCTGATACCAACCCAGAGCTAGCAGTTGAAGATGATAATCAATCCTTCCATTTGCAAGATGAAG
AGGATTATCTACATAACGAAGGCGATGCTAACGTGAAGGGGATGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.877

92.268

0.626
  pilU Acinetobacter baylyi ADP1

61.111

92.784

0.567
  pilU Vibrio cholerae strain A1552

54.857

90.206

0.495
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.058

88.918

0.392
  pilT Acinetobacter baylyi ADP1

44.444

88.144

0.392
  pilT Acinetobacter nosocomialis M2

43.567

88.144

0.384
  pilT Acinetobacter baumannii D1279779

43.567

88.144

0.384
  pilT Acinetobacter baumannii strain A118

43.567

88.144

0.384
  pilT Pseudomonas stutzeri DSM 10701

42.319

88.918

0.376
  pilT Pseudomonas aeruginosa PAK

42.319

88.918

0.376

Multiple sequence alignment