Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACMXYN_RS01470 Genome accession   NZ_CP180545
Coordinates   332146..333180 (+) Length   344 a.a.
NCBI ID   WP_415893176.1    Uniprot ID   -
Organism   Neptuniibacter sp. PT8_73     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 327146..338180
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMXYN_RS01440 (ACMXYN_01440) - 327351..327800 (-) 450 WP_415893170.1 DUF4426 domain-containing protein -
  ACMXYN_RS01445 (ACMXYN_01445) metW 327813..328403 (-) 591 WP_415893171.1 methionine biosynthesis protein MetW -
  ACMXYN_RS01450 (ACMXYN_01450) metX 328403..329572 (-) 1170 WP_415893172.1 homoserine O-succinyltransferase MetX -
  ACMXYN_RS01455 (ACMXYN_01455) - 329869..330414 (-) 546 WP_415893173.1 YggT family protein -
  ACMXYN_RS01460 (ACMXYN_01460) proC 330513..331340 (-) 828 WP_415893174.1 pyrroline-5-carboxylate reductase -
  ACMXYN_RS01465 (ACMXYN_01465) - 331359..332054 (-) 696 WP_415893175.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACMXYN_RS01470 (ACMXYN_01470) pilT 332146..333180 (+) 1035 WP_415893176.1 type IV pilus twitching motility protein PilT Machinery gene
  ACMXYN_RS01475 (ACMXYN_01475) pilU 333203..334369 (+) 1167 WP_415893177.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACMXYN_RS01480 (ACMXYN_01480) - 334446..335720 (-) 1275 WP_415893178.1 dihydroorotase -
  ACMXYN_RS01485 (ACMXYN_01485) - 335726..336730 (-) 1005 WP_415893179.1 aspartate carbamoyltransferase catalytic subunit -
  ACMXYN_RS01490 (ACMXYN_01490) pyrR 336740..337255 (-) 516 WP_415893180.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  ACMXYN_RS01495 (ACMXYN_01495) ruvX 337236..337712 (-) 477 WP_415893181.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38228.87 Da        Isoelectric Point: 6.5797

>NTDB_id=1096369 ACMXYN_RS01470 WP_415893176.1 332146..333180(+) (pilT) [Neptuniibacter sp. PT8_73]
MDITELLSFTVQQGASDLHISAGMPPIIRVDGDVRRIKLPSLDHKQVHTLVYDIMNDKQRKEYEDVLETDFSFEVPGLAR
FRVNAFNQNRGAGAVFRTIPSKVLTLDDLDMGKVFQDLSEKPRGLVLVTGPTGSGKSTTLAAMIDYINDTRADHILTIED
PIEFVHESKKCLVNQREVHRDTHSFAHALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAAEKDMVRSMLSESLMGVISQTLLKKPKGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGAAFGMKTLDQSL
QELMQKGLISRETARERAANPQQF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1096369 ACMXYN_RS01470 WP_415893176.1 332146..333180(+) (pilT) [Neptuniibacter sp. PT8_73]
ATGGATATTACCGAACTTTTATCATTTACCGTACAACAAGGCGCATCTGACTTGCATATCTCTGCAGGGATGCCTCCTAT
TATTCGTGTTGACGGTGATGTGCGTCGCATTAAACTGCCTTCATTGGATCATAAGCAGGTCCACACACTCGTATACGACA
TTATGAACGATAAACAACGTAAAGAGTACGAAGATGTGCTTGAAACGGATTTTTCGTTCGAAGTGCCTGGTTTGGCACGT
TTTCGTGTCAATGCATTTAACCAGAACCGTGGTGCTGGTGCTGTTTTCCGTACGATCCCGAGCAAGGTATTGACCCTAGA
TGATCTTGATATGGGTAAGGTTTTTCAGGACTTGTCTGAAAAACCGCGTGGTTTAGTGCTTGTGACGGGGCCAACGGGTT
CCGGTAAAAGTACCACCTTGGCTGCAATGATTGATTACATCAATGATACTCGCGCAGATCACATTCTTACAATCGAAGAT
CCAATCGAATTCGTGCATGAAAGTAAAAAGTGCTTGGTTAACCAGCGTGAAGTGCATCGAGATACTCATAGCTTTGCACA
TGCGCTTCGATCGGCACTTCGTGAAGACCCGGATATTATCCTTGTAGGTGAGATGCGAGACTTGGAAACTATCCGTCTTG
CGCTTACGGCTGCGGAAACAGGTCACTTAGTGTTTGGTACTTTGCATACGACCTCTGCAGCAAAAACAATTGACCGTGTG
ATTGACGTATTTCCAGCGGCTGAGAAAGATATGGTTCGATCCATGCTTTCAGAATCACTGATGGGCGTTATATCTCAAAC
ACTTTTGAAAAAGCCGAAAGGCGGACGTGTTGCAGCTCACGAAATTATGATCGGTACACCAGCTATTCGTAACCTTATTC
GTGAGGATAAAGTGGCGCAGATGTATTCTGCGATCCAGACGGGCGCTGCATTTGGTATGAAAACCTTGGATCAATCTCTA
CAAGAGTTGATGCAGAAAGGACTAATCTCTCGCGAGACAGCGCGTGAGCGAGCAGCAAACCCACAACAGTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

82.267

100

0.823
  pilT Pseudomonas stutzeri DSM 10701

81.395

100

0.814
  pilT Acinetobacter nosocomialis M2

81.105

100

0.811
  pilT Acinetobacter baumannii D1279779

81.105

100

0.811
  pilT Acinetobacter baumannii strain A118

81.105

100

0.811
  pilT Acinetobacter baylyi ADP1

79.651

100

0.797
  pilT Legionella pneumophila strain Lp02

73.256

100

0.733
  pilT Legionella pneumophila strain ERS1305867

73.256

100

0.733
  pilT Neisseria meningitidis 8013

67.638

99.709

0.674
  pilT Neisseria gonorrhoeae MS11

67.347

99.709

0.672
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.062

97.965

0.657
  pilT Vibrio cholerae strain A1552

67.062

97.965

0.657
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

100

0.509
  pilU Pseudomonas stutzeri DSM 10701

41.791

97.384

0.407
  pilU Vibrio cholerae strain A1552

41.493

97.384

0.404
  pilU Acinetobacter baylyi ADP1

39.017

100

0.392

Multiple sequence alignment