Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACMXYN_RS01475 Genome accession   NZ_CP180545
Coordinates   333203..334369 (+) Length   388 a.a.
NCBI ID   WP_415893177.1    Uniprot ID   -
Organism   Neptuniibacter sp. PT8_73     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 328203..339369
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMXYN_RS01450 (ACMXYN_01450) metX 328403..329572 (-) 1170 WP_415893172.1 homoserine O-succinyltransferase MetX -
  ACMXYN_RS01455 (ACMXYN_01455) - 329869..330414 (-) 546 WP_415893173.1 YggT family protein -
  ACMXYN_RS01460 (ACMXYN_01460) proC 330513..331340 (-) 828 WP_415893174.1 pyrroline-5-carboxylate reductase -
  ACMXYN_RS01465 (ACMXYN_01465) - 331359..332054 (-) 696 WP_415893175.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACMXYN_RS01470 (ACMXYN_01470) pilT 332146..333180 (+) 1035 WP_415893176.1 type IV pilus twitching motility protein PilT Machinery gene
  ACMXYN_RS01475 (ACMXYN_01475) pilU 333203..334369 (+) 1167 WP_415893177.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACMXYN_RS01480 (ACMXYN_01480) - 334446..335720 (-) 1275 WP_415893178.1 dihydroorotase -
  ACMXYN_RS01485 (ACMXYN_01485) - 335726..336730 (-) 1005 WP_415893179.1 aspartate carbamoyltransferase catalytic subunit -
  ACMXYN_RS01490 (ACMXYN_01490) pyrR 336740..337255 (-) 516 WP_415893180.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  ACMXYN_RS01495 (ACMXYN_01495) ruvX 337236..337712 (-) 477 WP_415893181.1 Holliday junction resolvase RuvX -
  ACMXYN_RS01500 (ACMXYN_01500) - 337766..338338 (-) 573 WP_415893182.1 YqgE/AlgH family protein -
  ACMXYN_RS01505 (ACMXYN_01505) - 338376..339308 (-) 933 WP_415893183.1 TonB family protein -

Sequence

Protein


Download         Length: 388 a.a.        Molecular weight: 43398.46 Da        Isoelectric Point: 6.0160

>NTDB_id=1096370 ACMXYN_RS01475 WP_415893177.1 333203..334369(+) (pilU) [Neptuniibacter sp. PT8_73]
MDFTQLLKVMVERDASDLFVTAGARPTIKVDGTLKPLTKDTLKPSQARALVYSTMNDKQLAEFEGSHECNFAISAPGLGR
FRVSAFFQRNSAGMVLRKINNYIPSLEELNLPPILRDLSMTKRGLILFVGGTSTGKSTSLASMIDYRNTNHRGHIITIED
PIEYIHEHKQSIITQREVGIDTDSFDVALKNTLRQAPDVILMGEIRTRETMQHGIVFAETGHLTLATLHANNANQALDRV
ISFFPPEHHDQLWMDLSLNLKAIIAQQLLPTKDGKGRRAAIEVLINTPLIQDLIRKGEVHEIKDVVKKSTNLGMQTFDQS
LFTLYKDGVITYDVALAHADSPNDLRLMIKLSADTNPELAVEDDNQSFHLQDEEDYLHNEGDANVKGM

Nucleotide


Download         Length: 1167 bp        

>NTDB_id=1096370 ACMXYN_RS01475 WP_415893177.1 333203..334369(+) (pilU) [Neptuniibacter sp. PT8_73]
GTGGATTTTACGCAGCTACTAAAGGTTATGGTAGAGAGGGATGCATCTGACCTTTTTGTTACGGCCGGTGCAAGGCCTAC
GATTAAAGTTGATGGTACTTTGAAGCCGTTAACAAAAGACACCCTCAAGCCTTCTCAGGCACGGGCGCTTGTATACAGTA
CCATGAATGATAAGCAGCTTGCTGAGTTTGAAGGATCACATGAGTGTAACTTTGCGATTAGTGCGCCGGGTCTAGGTCGT
TTCCGTGTGTCTGCATTCTTTCAGCGTAACTCTGCGGGGATGGTATTAAGGAAAATTAATAACTATATCCCGAGTTTAGA
AGAGCTCAATTTGCCACCGATTCTCCGTGATTTGTCGATGACAAAACGAGGACTGATCTTGTTTGTGGGTGGTACCTCTA
CAGGTAAATCGACTTCCTTGGCATCGATGATTGATTACCGTAATACCAATCACCGTGGTCATATCATTACCATCGAAGAC
CCGATTGAATATATCCATGAGCACAAGCAAAGTATTATCACTCAGCGTGAAGTGGGTATCGATACAGACTCGTTTGATGT
GGCGTTGAAAAATACGCTGCGTCAGGCGCCTGATGTAATTTTGATGGGTGAGATCCGTACTCGTGAAACGATGCAGCACG
GTATCGTATTTGCCGAAACAGGCCACTTAACTTTAGCGACATTGCACGCTAACAATGCTAACCAGGCATTGGATCGTGTT
ATCAGCTTTTTCCCACCTGAACACCACGATCAGTTGTGGATGGATCTGTCGCTGAACTTGAAAGCGATCATAGCCCAGCA
ACTTTTGCCGACGAAAGATGGTAAAGGTCGTCGTGCAGCGATAGAGGTATTGATTAATACGCCATTGATTCAGGACCTTA
TCCGCAAGGGCGAAGTTCATGAGATCAAAGATGTAGTTAAGAAATCGACTAACTTAGGGATGCAGACGTTCGATCAGTCC
TTATTTACTCTCTATAAAGATGGTGTGATTACTTACGATGTGGCGTTGGCTCATGCGGATTCACCAAACGATCTCCGTCT
CATGATTAAGTTGAGTGCTGATACCAACCCAGAGCTAGCAGTTGAAGACGATAATCAATCCTTCCATTTGCAAGATGAAG
AGGATTATCTACATAACGAAGGCGATGCTAACGTTAAGGGGATGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.156

92.268

0.629
  pilU Acinetobacter baylyi ADP1

61.111

92.784

0.567
  pilU Vibrio cholerae strain A1552

54.857

90.206

0.495
  pilT Acinetobacter baylyi ADP1

44.444

88.144

0.392
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.768

88.918

0.389
  pilT Acinetobacter nosocomialis M2

43.567

88.144

0.384
  pilT Acinetobacter baumannii D1279779

43.567

88.144

0.384
  pilT Acinetobacter baumannii strain A118

43.567

88.144

0.384
  pilT Pseudomonas stutzeri DSM 10701

42.319

88.918

0.376
  pilT Pseudomonas aeruginosa PAK

42.319

88.918

0.376

Multiple sequence alignment