Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACMXYV_RS01430 Genome accession   NZ_CP180543
Coordinates   317125..318159 (+) Length   344 a.a.
NCBI ID   WP_415890005.1    Uniprot ID   -
Organism   Neptuniibacter sp. SY11_33     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 312319..326051 317125..318159 within 0


Gene organization within MGE regions


Location: 312319..326051
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMXYV_RS01400 (ACMXYV_01400) - 312319..312768 (-) 450 WP_415889999.1 DUF4426 domain-containing protein -
  ACMXYV_RS01405 (ACMXYV_01405) metW 312781..313371 (-) 591 WP_415890000.1 methionine biosynthesis protein MetW -
  ACMXYV_RS01410 (ACMXYV_01410) metX 313371..314540 (-) 1170 WP_415890001.1 homoserine O-succinyltransferase MetX -
  ACMXYV_RS01415 (ACMXYV_01415) - 314837..315382 (-) 546 WP_415890002.1 YggT family protein -
  ACMXYV_RS01420 (ACMXYV_01420) proC 315492..316319 (-) 828 WP_415890003.1 pyrroline-5-carboxylate reductase -
  ACMXYV_RS01425 (ACMXYV_01425) - 316338..317033 (-) 696 WP_415890004.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACMXYV_RS01430 (ACMXYV_01430) pilT 317125..318159 (+) 1035 WP_415890005.1 type IV pilus twitching motility protein PilT Machinery gene
  ACMXYV_RS01435 (ACMXYV_01435) pilU 318182..319348 (+) 1167 WP_415890006.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACMXYV_RS01440 (ACMXYV_01440) - 319426..320700 (-) 1275 WP_415890008.1 dihydroorotase -
  ACMXYV_RS01445 (ACMXYV_01445) - 320706..321710 (-) 1005 WP_415890009.1 aspartate carbamoyltransferase catalytic subunit -
  ACMXYV_RS01450 (ACMXYV_01450) pyrR 321720..322235 (-) 516 WP_415890011.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  ACMXYV_RS01455 (ACMXYV_01455) ruvX 322216..322692 (-) 477 WP_415890012.1 Holliday junction resolvase RuvX -
  ACMXYV_RS01460 (ACMXYV_01460) - 322746..323318 (-) 573 WP_415890013.1 YqgE/AlgH family protein -
  ACMXYV_RS01465 (ACMXYV_01465) - 323356..324288 (-) 933 WP_415890014.1 TonB family protein -
  ACMXYV_RS01470 (ACMXYV_01470) gshB 324319..325272 (-) 954 WP_415890015.1 glutathione synthase -
  ACMXYV_RS01475 (ACMXYV_01475) - 325323..325994 (-) 672 WP_415890017.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38244.87 Da        Isoelectric Point: 6.5797

>NTDB_id=1096324 ACMXYV_RS01430 WP_415890005.1 317125..318159(+) (pilT) [Neptuniibacter sp. SY11_33]
MDITELLSFTVQQGASDLHISAGMPPIIRVDGDVRRIKLPSLDHKQVHTLVYDIMNDKQRKEYEDVLETDFSFEVPGLAR
FRVNAFNQNRGAGAVFRTIPSKVLTLDDLDMGKVFQDLSEKPRGLVLVTGPTGSGKSTTLAAMIDYINDTRADHILTIED
PIEFVHESKKCLVNQREVHRDTHSFSHALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAAEKDMVRSMLSESLMGVISQTLLKKPKGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGAAFGMKTLDQSL
QELMQKGLISRETARERAANPQQF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1096324 ACMXYV_RS01430 WP_415890005.1 317125..318159(+) (pilT) [Neptuniibacter sp. SY11_33]
ATGGATATTACCGAACTTTTATCATTTACCGTACAACAAGGTGCATCTGACTTGCATATATCTGCAGGTATGCCTCCTAT
TATTCGTGTGGACGGTGATGTGCGTCGCATAAAGCTGCCTTCATTGGATCATAAGCAGGTCCACACACTCGTATATGACA
TCATGAACGATAAACAACGTAAAGAGTACGAAGATGTACTCGAAACGGATTTTTCGTTCGAAGTGCCTGGTTTAGCGCGT
TTTCGTGTCAATGCGTTTAACCAGAACCGTGGTGCCGGTGCTGTTTTTCGTACGATTCCGAGCAAGGTGTTGACCCTAGA
TGATCTTGATATGGGTAAGGTTTTTCAGGATCTATCTGAAAAACCGCGTGGATTGGTACTTGTAACGGGGCCAACGGGTT
CCGGTAAAAGTACCACCTTGGCTGCAATGATTGATTACATCAATGATACTCGTGCAGATCATATTCTTACGATCGAAGAT
CCAATCGAATTCGTGCATGAAAGTAAGAAATGTTTGGTTAACCAGCGTGAAGTTCATCGAGACACCCATAGCTTTTCACA
TGCGCTTCGTTCGGCATTGCGTGAAGACCCGGATATTATTCTTGTAGGTGAGATGCGAGATCTAGAAACCATCCGCCTTG
CATTGACTGCAGCAGAAACTGGCCATTTAGTATTTGGTACATTGCATACCACTTCAGCAGCAAAAACTATTGACCGTGTG
ATTGACGTATTCCCAGCGGCTGAGAAGGATATGGTGAGATCCATGCTTTCAGAATCACTGATGGGCGTTATATCGCAAAC
ACTGTTGAAAAAGCCGAAAGGCGGACGAGTTGCTGCTCATGAAATTATGATCGGTACCCCAGCTATTCGTAACCTTATCC
GCGAGGATAAAGTGGCACAAATGTATTCAGCGATCCAGACGGGTGCAGCATTTGGTATGAAAACCTTGGATCAATCTCTA
CAAGAGTTGATGCAGAAAGGACTAATCTCTCGCGAGACAGCGCGTGAGCGAGCAGCAAACCCACAACAGTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

82.558

100

0.826
  pilT Pseudomonas stutzeri DSM 10701

81.686

100

0.817
  pilT Acinetobacter nosocomialis M2

81.105

100

0.811
  pilT Acinetobacter baumannii D1279779

81.105

100

0.811
  pilT Acinetobacter baumannii strain A118

81.105

100

0.811
  pilT Acinetobacter baylyi ADP1

79.651

100

0.797
  pilT Legionella pneumophila strain Lp02

73.256

100

0.733
  pilT Legionella pneumophila strain ERS1305867

73.256

100

0.733
  pilT Neisseria meningitidis 8013

67.347

99.709

0.672
  pilT Neisseria gonorrhoeae MS11

67.055

99.709

0.669
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.062

97.965

0.657
  pilT Vibrio cholerae strain A1552

67.062

97.965

0.657
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

100

0.509
  pilU Pseudomonas stutzeri DSM 10701

41.791

97.384

0.407
  pilU Vibrio cholerae strain A1552

41.493

97.384

0.404
  pilU Acinetobacter baylyi ADP1

39.017

100

0.392

Multiple sequence alignment