Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACMXYV_RS01435 Genome accession   NZ_CP180543
Coordinates   318182..319348 (+) Length   388 a.a.
NCBI ID   WP_415890006.1    Uniprot ID   -
Organism   Neptuniibacter sp. SY11_33     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 312319..326051 318182..319348 within 0


Gene organization within MGE regions


Location: 312319..326051
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMXYV_RS01400 (ACMXYV_01400) - 312319..312768 (-) 450 WP_415889999.1 DUF4426 domain-containing protein -
  ACMXYV_RS01405 (ACMXYV_01405) metW 312781..313371 (-) 591 WP_415890000.1 methionine biosynthesis protein MetW -
  ACMXYV_RS01410 (ACMXYV_01410) metX 313371..314540 (-) 1170 WP_415890001.1 homoserine O-succinyltransferase MetX -
  ACMXYV_RS01415 (ACMXYV_01415) - 314837..315382 (-) 546 WP_415890002.1 YggT family protein -
  ACMXYV_RS01420 (ACMXYV_01420) proC 315492..316319 (-) 828 WP_415890003.1 pyrroline-5-carboxylate reductase -
  ACMXYV_RS01425 (ACMXYV_01425) - 316338..317033 (-) 696 WP_415890004.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACMXYV_RS01430 (ACMXYV_01430) pilT 317125..318159 (+) 1035 WP_415890005.1 type IV pilus twitching motility protein PilT Machinery gene
  ACMXYV_RS01435 (ACMXYV_01435) pilU 318182..319348 (+) 1167 WP_415890006.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACMXYV_RS01440 (ACMXYV_01440) - 319426..320700 (-) 1275 WP_415890008.1 dihydroorotase -
  ACMXYV_RS01445 (ACMXYV_01445) - 320706..321710 (-) 1005 WP_415890009.1 aspartate carbamoyltransferase catalytic subunit -
  ACMXYV_RS01450 (ACMXYV_01450) pyrR 321720..322235 (-) 516 WP_415890011.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  ACMXYV_RS01455 (ACMXYV_01455) ruvX 322216..322692 (-) 477 WP_415890012.1 Holliday junction resolvase RuvX -
  ACMXYV_RS01460 (ACMXYV_01460) - 322746..323318 (-) 573 WP_415890013.1 YqgE/AlgH family protein -
  ACMXYV_RS01465 (ACMXYV_01465) - 323356..324288 (-) 933 WP_415890014.1 TonB family protein -
  ACMXYV_RS01470 (ACMXYV_01470) gshB 324319..325272 (-) 954 WP_415890015.1 glutathione synthase -
  ACMXYV_RS01475 (ACMXYV_01475) - 325323..325994 (-) 672 WP_415890017.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 388 a.a.        Molecular weight: 43422.48 Da        Isoelectric Point: 5.9012

>NTDB_id=1096325 ACMXYV_RS01435 WP_415890006.1 318182..319348(+) (pilU) [Neptuniibacter sp. SY11_33]
MDFTQLLKVMVERDASDLFVTAGARPTIKVDGTLKPLTKDTLKPSQARALVYSTMNDKQLAEFEGSHECNFAISAPGLGR
FRVSAFFQRNSAGMVLRKINNYIPSLEELNLPPILRDLSMTKRGLILFVGGTSTGKSTSLASMIDYRNTNHRGHIITIED
PIEYIHEHKQSIITQREVGIDTDSFDVALKNTLRQAPDVILMGEIRTRETMQHGIVFAETGHLTLATLHANNANQALDRV
ISFFPPEHHDQLWMDLSLNLKAIIAQQLLPTKDGKGRRAAIEVLINTPLIQDLIRKGEVHEIKDVVKKSTNLGMQTFDQS
LFTLYKDGVITYDVALAHADSPNDLRLMIKLSADTNPELAPDEDNQSFYLQDEEDYLHNEGDANVKGM

Nucleotide


Download         Length: 1167 bp        

>NTDB_id=1096325 ACMXYV_RS01435 WP_415890006.1 318182..319348(+) (pilU) [Neptuniibacter sp. SY11_33]
GTGGATTTTACGCAGCTACTAAAGGTTATGGTAGAGAGGGATGCATCTGACCTTTTTGTTACGGCCGGTGCAAGGCCTAC
GATTAAAGTTGACGGTACTTTGAAGCCGTTAACAAAAGACACACTCAAGCCTTCCCAGGCGCGGGCGCTTGTTTACAGTA
CCATGAATGATAAACAGCTTGCTGAGTTTGAAGGATCACATGAGTGTAACTTTGCGATTAGTGCACCAGGTCTAGGTCGT
TTCCGTGTGTCTGCATTCTTTCAGCGTAACTCAGCGGGGATGGTATTAAGGAAGATTAATAACTACATCCCGAGTTTAGA
AGAACTCAACTTGCCACCAATCCTTCGTGATTTGTCGATGACAAAGCGCGGCTTGATCTTATTTGTGGGTGGTACATCCA
CGGGTAAATCGACCTCTTTGGCGTCGATGATTGATTACCGTAATACCAATCATCGTGGTCATATCATTACCATCGAAGAC
CCGATTGAGTATATCCACGAGCATAAGCAAAGTATCATCACCCAGCGTGAAGTAGGTATCGATACAGACTCGTTTGATGT
GGCGTTGAAAAATACGCTTCGTCAGGCACCTGATGTAATTTTGATGGGTGAGATCCGTACTCGAGAAACGATGCAGCACG
GTATCGTATTTGCCGAAACGGGCCACTTAACTTTGGCGACATTGCACGCTAATAATGCTAACCAAGCATTGGATCGAGTA
ATCAGCTTCTTCCCACCTGAGCACCATGATCAGCTGTGGATGGATCTGTCACTGAACTTGAAAGCTATCATAGCGCAGCA
GCTTTTGCCGACCAAAGATGGGAAAGGTCGTCGTGCAGCGATCGAGGTGTTGATTAATACACCACTGATTCAGGATCTTA
TCCGTAAGGGTGAAGTTCATGAGATCAAAGATGTAGTTAAGAAATCGACTAACTTAGGGATGCAGACGTTCGATCAGTCC
CTGTTTACTCTCTATAAAGATGGCGTGATTACCTACGATGTAGCCTTAGCCCATGCGGATTCACCAAACGATCTCCGCCT
AATGATTAAGCTGAGTGCTGATACGAATCCAGAATTGGCGCCGGACGAAGATAATCAGTCTTTCTATCTACAGGATGAAG
AGGATTACCTACATAACGAAGGCGATGCTAACGTTAAGGGGATGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.156

92.268

0.629
  pilU Acinetobacter baylyi ADP1

61.111

92.784

0.567
  pilU Vibrio cholerae strain A1552

54.857

90.206

0.495
  pilT Acinetobacter baylyi ADP1

44.444

88.144

0.392
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.768

88.918

0.389
  pilT Acinetobacter nosocomialis M2

43.567

88.144

0.384
  pilT Acinetobacter baumannii D1279779

43.567

88.144

0.384
  pilT Acinetobacter baumannii strain A118

43.567

88.144

0.384
  pilT Pseudomonas stutzeri DSM 10701

42.319

88.918

0.376
  pilT Pseudomonas aeruginosa PAK

42.319

88.918

0.376

Multiple sequence alignment