Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACMZ68_RS04215 Genome accession   NZ_CP180522
Coordinates   878565..879602 (-) Length   345 a.a.
NCBI ID   WP_002121749.1    Uniprot ID   A0A009I411
Organism   Acinetobacter pittii strain AP5091     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 873565..884602
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMZ68_RS04185 (ACMZ68_04185) - 874355..874810 (+) 456 WP_415871776.1 bacteriohemerythrin -
  ACMZ68_RS04190 (ACMZ68_04190) - 874964..876031 (+) 1068 WP_415871777.1 hypothetical protein -
  ACMZ68_RS04195 (ACMZ68_04195) - 876031..876345 (+) 315 WP_017398881.1 RnfH family protein -
  ACMZ68_RS04200 (ACMZ68_04200) omlA 876410..876808 (-) 399 WP_002121740.1 outer membrane protein assembly factor BamE -
  ACMZ68_RS04205 (ACMZ68_04205) fur 876920..877357 (+) 438 WP_002121758.1 ferric iron uptake transcriptional regulator -
  ACMZ68_RS04210 (ACMZ68_04210) pilU 877427..878545 (-) 1119 WP_032004223.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACMZ68_RS04215 (ACMZ68_04215) pilT 878565..879602 (-) 1038 WP_002121749.1 type IV pilus twitching motility protein PilT Machinery gene
  ACMZ68_RS04220 (ACMZ68_04220) yggS 879729..880421 (+) 693 WP_415871778.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACMZ68_RS04225 (ACMZ68_04225) sbcC 880622..884218 (-) 3597 WP_415871779.1 AAA family ATPase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38389.97 Da        Isoelectric Point: 6.7269

>NTDB_id=1096183 ACMZ68_RS04215 WP_002121749.1 878565..879602(-) (pilT) [Acinetobacter pittii strain AP5091]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALDHKDVHRLVYDIMNDKQRRDYEEKLETDFSFEVPNVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGQIFKDICDYPRGIVLVTGPTGSGKSTTLAAMMDYINENRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANHGMTTLDQSL
KNLVSRGVISPQTARTAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1096183 ACMZ68_RS04215 WP_002121749.1 878565..879602(-) (pilT) [Acinetobacter pittii strain AP5091]
ATGGATATTACAGAGCTACTCGCCTTCTCTGTGAAAAATGGCGCGTCCGATTTACATTTGTCTGCTGGCATGCCACCAAT
GATTCGCGTTGATGGTGAGGTCCGTCGTATTAACTTACCTGCTCTAGATCATAAAGATGTTCATCGTCTGGTCTACGACA
TCATGAATGATAAACAGCGTCGTGACTACGAAGAAAAGCTTGAAACCGACTTTTCTTTTGAAGTACCCAACGTTGCGCGT
TTCCGTGTTAACGCATTTAATCAAAACCGTGGTGCTGGTGCAGTATTTCGTACCATTCCTTCCAAAGTACTCACCATGGA
AGATTTAGGCTTAGGTCAAATCTTTAAAGATATTTGTGACTACCCACGCGGCATCGTATTAGTTACGGGCCCAACAGGTT
CTGGTAAATCGACCACACTTGCAGCGATGATGGACTACATCAACGAAAACCGTTATGACCACATTTTAACGGTTGAAGAC
CCTATCGAGTTTGTACACCAGTCTAAGAAATGTCTGATTAACCAACGTGAAGTGCACCGTGATACACACGGCTTTAATGA
GGCGCTACGTTCAGCACTGCGTGAAGACCCAGATATTATTTTGGTCGGTGAGATGCGTGACCTTGAGACTATTCGTTTAG
CGCTTACTGCTGCCGAAACAGGTCATTTGGTATTTGGTACACTTCATACCACTTCTGCTGCGAAAACCATTGACCGTGTA
ATTGACGTATTCCCAGCCGAAGAAAAAGATATGGTCCGTGCCATGCTCTCTGAATCTTTACAGGCGGTTATTTCACAAAC
CCTGCTTAAAAAGAATGGCGGCGGTCGTGTCGCAGCTCATGAAATCATGATTGGTATTCCTGCCATTCGTAACTTAATTC
GTGAAAATAAAGTCGCGCAAATGTACTCAGCTATTCAGACAGGCGCAAATCATGGCATGACCACCCTCGACCAAAGCTTA
AAAAATTTGGTATCTCGCGGTGTGATCAGCCCACAAACCGCTCGTACTGCTGCTAAACAGCCTGAATCATTTCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A009I411

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

99.13

100

0.991
  pilT Acinetobacter baumannii D1279779

98.841

100

0.988
  pilT Acinetobacter baumannii strain A118

98.841

100

0.988
  pilT Acinetobacter baylyi ADP1

88.663

99.71

0.884
  pilT Pseudomonas stutzeri DSM 10701

83.43

99.71

0.832
  pilT Pseudomonas aeruginosa PAK

83.14

99.71

0.829
  pilT Legionella pneumophila strain Lp02

75.291

99.71

0.751
  pilT Legionella pneumophila strain ERS1305867

75.291

99.71

0.751
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.429

97.391

0.696
  pilT Vibrio cholerae strain A1552

71.429

97.391

0.696
  pilT Neisseria meningitidis 8013

68.116

100

0.681
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.678
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

100

0.519
  pilU Vibrio cholerae strain A1552

41.593

98.261

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.101

0.397
  pilU Acinetobacter baylyi ADP1

39.017

100

0.391

Multiple sequence alignment