Detailed information NTG1096182

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACMZ68_RS04210
• Genome accession: NZ_CP180522
• Coordinates: 877427..878545 (-)
• Length: 372 a.a.
• NCBI ID: WP_032004223.1
• Uniprot ID: A0A010IQ02
• Organism: Acinetobacter pittii strain AP5091
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 872427..883545

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACMZ68_RS04180 (ACMZ68_04180)	-	873255..874103 (+)	849	WP_415872180.1	class II glutamine amidotransferase	-
ACMZ68_RS04185 (ACMZ68_04185)	-	874355..874810 (+)	456	WP_415871776.1	bacteriohemerythrin	-
ACMZ68_RS04190 (ACMZ68_04190)	-	874964..876031 (+)	1068	WP_415871777.1	hypothetical protein	-
ACMZ68_RS04195 (ACMZ68_04195)	-	876031..876345 (+)	315	WP_017398881.1	RnfH family protein	-
ACMZ68_RS04200 (ACMZ68_04200)	omlA	876410..876808 (-)	399	WP_002121740.1	outer membrane protein assembly factor BamE	-
ACMZ68_RS04205 (ACMZ68_04205)	fur	876920..877357 (+)	438	WP_002121758.1	ferric iron uptake transcriptional regulator	-
ACMZ68_RS04210 (ACMZ68_04210)	pilU	877427..878545 (-)	1119	WP_032004223.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACMZ68_RS04215 (ACMZ68_04215)	pilT	878565..879602 (-)	1038	WP_002121749.1	type IV pilus twitching motility protein PilT	Machinery gene
ACMZ68_RS04220 (ACMZ68_04220)	yggS	879729..880421 (+)	693	WP_415871778.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41917.12 Da
• Isoelectric Point: 6.6403

>NTDB_id=1096182 ACMZ68_RS04210 WP_032004223.1 877427..878545(-) (pilU) [Acinetobacter pittii strain AP5091]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEIIGQLLHSIMSEKQRKEFADTRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISYRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKSGQITYKDALKHADSPNDLRLTIKLAEEGPEQIAGTNQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=1096182 ACMZ68_RS04210 WP_032004223.1 877427..878545(-) (pilU) [Acinetobacter pittii strain AP5091]
ATGGATTTTAATGACCTGCTCAACCTGATGGTTGAAAAAAAATCATCCGACCTCTTTATTACAGATGGCGTTGCACCATC
GATGAAGATTAACGGGCAAATTGTTCCCATTTCAAAAAATAGTCTTTCAGGCGAAATTATCGGGCAACTATTACACTCCA
TCATGAGTGAAAAACAGCGCAAAGAATTTGCGGACACTCGTGAATGTAACTTTGCCATTATGAATCGTGATAAAACTGCC
CGCTTTCGTGTGAGTGCTTTTCAACAGCGTGATATGCCCGGCATGGTACTACGTCGAATTGAAACTAAAATTCCTTCAAT
TGATGACTTGCAGTTACCACCTGTGCTTAAAGATTTATCGATGACTAAACGCGGCATCATTATTTTTGTAGGTGCAACAG
GTACAGGTAAATCGACATCATTGGCTTCAATGATTAGCTATCGTAACCATAACTCTAAAGGTCATATCATTACCATTGAA
GACCCTATCGAGTTTATTCATGAACATGCTGGCTGCATCATTACCCAACGTGAAGTCGGGATCGATACAGACTCATTCGA
AATTGCCTTAAAGAATACGTTGCGACAAGCACCCGATGTGATCTTAATTGGTGAGATTCGCTCTCGTGAAGTTATGGACT
ATGCGATTGGTTTTGCCGAAACGGGTCACTTGGTATTAGCCACAATGCATGCGAACAATGCCAACCAAGCACTTGACCGT
ATTATTCACTTCTTTGAAAGTGACCGTCATAGCCAGCTCTACATGGATTTATCGCTTAATTTAAAGGCCATGATTGCACA
GCAACTTATTCCGACTCCAGATGGTAATTCTCGCCGTGCAGCGATTGAGATTCTCATTAACTCCCCATTAATTTCAGACT
ATATTCGTAAAGGTGAAATTCATGAAATTAAAGATTTAATGAAACGCTCACGTGAACTCGGTATGCAAACTTTTGACCAA
GCACTGTTTGATCTTTATAAGTCAGGCCAAATTACCTACAAAGATGCATTAAAACATGCCGACTCACCAAACGATTTACG
TTTAACAATTAAGCTTGCCGAAGAAGGTCCTGAGCAAATTGCAGGCACAAATCAGCATTTAACCTTCGACAGACAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (40-270)

3D structure

• AlphaFold DB: A0A010IQ02

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.397	100	0.766
pilU	Pseudomonas stutzeri DSM 10701	68.464	99.731	0.683
pilU	Vibrio cholerae strain A1552	53.784	99.462	0.535
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.659	97.849	0.398
pilT	Legionella pneumophila strain Lp02	39.645	90.86	0.36
pilT	Legionella pneumophila strain ERS1305867	39.645	90.86	0.36