Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACMYQ1_RS15565 Genome accession   NZ_CP180478
Coordinates   3592400..3593437 (-) Length   345 a.a.
NCBI ID   WP_006082517.1    Uniprot ID   A0AA50Q7P7
Organism   Shewanella oncorhynchi strain XWfb1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3587400..3598437
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMYQ1_RS15530 (ACMYQ1_15530) - 3587505..3587822 (+) 318 WP_263162154.1 hypothetical protein -
  ACMYQ1_RS15535 (ACMYQ1_15535) yciH 3588001..3588330 (+) 330 WP_219251298.1 stress response translation initiation inhibitor YciH -
  ACMYQ1_RS15540 (ACMYQ1_15540) - 3588400..3588963 (+) 564 WP_263209620.1 YqgE/AlgH family protein -
  ACMYQ1_RS15545 (ACMYQ1_15545) ruvX 3588993..3589418 (+) 426 WP_028761279.1 Holliday junction resolvase RuvX -
  ACMYQ1_RS15550 (ACMYQ1_15550) hemH 3589506..3590519 (-) 1014 WP_393942004.1 ferrochelatase -
  ACMYQ1_RS15555 (ACMYQ1_15555) - 3590552..3591091 (-) 540 WP_393942006.1 glutathione peroxidase -
  ACMYQ1_RS15560 (ACMYQ1_15560) pilU 3591278..3592390 (-) 1113 WP_028761282.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACMYQ1_RS15565 (ACMYQ1_15565) pilT 3592400..3593437 (-) 1038 WP_006082517.1 type IV pilus twitching motility protein PilT Machinery gene
  ACMYQ1_RS15570 (ACMYQ1_15570) - 3593473..3594171 (+) 699 WP_415775122.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACMYQ1_RS15575 (ACMYQ1_15575) proC 3594388..3595206 (+) 819 WP_393942011.1 pyrroline-5-carboxylate reductase -
  ACMYQ1_RS15580 (ACMYQ1_15580) - 3595253..3595801 (+) 549 WP_393942013.1 YggT family protein -
  ACMYQ1_RS15585 (ACMYQ1_15585) yggU 3595801..3596106 (+) 306 WP_219253476.1 DUF167 family protein YggU -
  ACMYQ1_RS15590 (ACMYQ1_15590) - 3596242..3596676 (+) 435 WP_113939419.1 DUF4426 domain-containing protein -
  ACMYQ1_RS15595 (ACMYQ1_15595) rdgB 3596799..3597416 (+) 618 WP_131523655.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38297.94 Da        Isoelectric Point: 6.9095

>NTDB_id=1096016 ACMYQ1_RS15565 WP_006082517.1 3592400..3593437(-) (pilT) [Shewanella oncorhynchi strain XWfb1]
MEITELLAFSVKHKASDLHLSAGISPMIRVDGEVRKINLPALDHQGVHSLVYDIMNDKQRKDYEEHLEIDFSFEVPNLAR
FRVNAFNQSRGAAAVFRTIPSDILSLEQLGAPEIFKKIASFPRGLVLVTGPTGSGKSTTLAAMVDYVNENRHDHILTIED
PIEFVHQNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAGEKDMVRTMLSESLQAVISQTLIKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLEQCL
QNLVNRGLITREDAMAKSSNKQATF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1096016 ACMYQ1_RS15565 WP_006082517.1 3592400..3593437(-) (pilT) [Shewanella oncorhynchi strain XWfb1]
ATGGAAATCACTGAGTTATTGGCATTTAGTGTAAAACACAAAGCCTCGGATCTACACCTTTCTGCAGGGATATCTCCCAT
GATCCGTGTTGACGGTGAAGTGAGAAAAATTAACCTGCCCGCGCTCGATCATCAAGGTGTACACAGTTTAGTGTACGACA
TAATGAATGATAAGCAGCGCAAGGACTACGAAGAACATTTAGAAATCGATTTCTCGTTCGAAGTCCCTAATCTTGCACGC
TTTCGTGTTAACGCATTCAATCAATCCCGTGGTGCAGCGGCTGTGTTTCGTACCATTCCAAGTGACATTTTGTCACTTGA
GCAATTAGGTGCGCCGGAAATTTTTAAAAAAATTGCCAGTTTCCCTCGCGGGTTGGTGCTAGTGACTGGACCTACGGGGT
CGGGTAAGAGTACCACACTTGCCGCTATGGTGGATTACGTGAATGAAAATCGACATGATCATATCTTAACCATTGAAGAT
CCTATCGAATTCGTTCACCAAAATAAGCAATGTTTGATTAACCAACGTGAAGTTCACCGCCATACCCACAGCTTTAATGC
GGCGCTTAGAAGCGCACTGCGTGAAGACCCAGACGTGATTTTAGTCGGTGAGATGCGTGACCTTGAGACCATCCGTTTGG
CTATGACCGCCGCTGAAACAGGCCACTTAGTGTTTGGTACCTTGCATACTACTTCTGCCGCGAAAACGATTGACCGTGTG
GTCGACGTATTCCCTGCTGGTGAAAAAGACATGGTGCGCACTATGTTGTCTGAATCGCTGCAAGCGGTTATTTCGCAAAC
CCTGATTAAAAAGGTGGGAGGAGGCCGTGTCGCCGCCCATGAAATCATGATGGGCACGCCCGCTATTCGCAACTTAATTC
GTGAAGACAAAGTGGCGCAGATGTATTCGGCAATTCAAACAGGTATGGCCCATGGAATGCAGACCTTAGAGCAATGTTTA
CAGAACTTAGTCAATCGCGGTCTTATCACTCGTGAAGATGCAATGGCGAAAAGTTCGAATAAACAAGCGACATTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

80.409

99.13

0.797
  pilT Acinetobacter baumannii D1279779

80.409

99.13

0.797
  pilT Acinetobacter baumannii strain A118

80.409

99.13

0.797
  pilT Acinetobacter baylyi ADP1

77.843

99.42

0.774
  pilT Pseudomonas aeruginosa PAK

78.698

97.971

0.771
  pilT Pseudomonas stutzeri DSM 10701

77.485

99.13

0.768
  pilT Legionella pneumophila strain Lp02

75.073

98.841

0.742
  pilT Legionella pneumophila strain ERS1305867

75.073

98.841

0.742
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

73.294

97.681

0.716
  pilT Vibrio cholerae strain A1552

73.294

97.681

0.716
  pilT Neisseria meningitidis 8013

69.767

99.71

0.696
  pilT Neisseria gonorrhoeae MS11

69.477

99.71

0.693
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.558

98.261

0.487
  pilU Pseudomonas stutzeri DSM 10701

41.983

99.42

0.417
  pilU Vibrio cholerae strain A1552

41.399

99.42

0.412
  pilU Acinetobacter baylyi ADP1

39.763

97.681

0.388

Multiple sequence alignment