Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACMYQ1_RS15560 Genome accession   NZ_CP180478
Coordinates   3591278..3592390 (-) Length   370 a.a.
NCBI ID   WP_028761282.1    Uniprot ID   -
Organism   Shewanella oncorhynchi strain XWfb1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3586278..3597390
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMYQ1_RS15525 (ACMYQ1_15525) - 3586764..3587324 (-) 561 WP_028761276.1 DUF4136 domain-containing protein -
  ACMYQ1_RS15530 (ACMYQ1_15530) - 3587505..3587822 (+) 318 WP_263162154.1 hypothetical protein -
  ACMYQ1_RS15535 (ACMYQ1_15535) yciH 3588001..3588330 (+) 330 WP_219251298.1 stress response translation initiation inhibitor YciH -
  ACMYQ1_RS15540 (ACMYQ1_15540) - 3588400..3588963 (+) 564 WP_263209620.1 YqgE/AlgH family protein -
  ACMYQ1_RS15545 (ACMYQ1_15545) ruvX 3588993..3589418 (+) 426 WP_028761279.1 Holliday junction resolvase RuvX -
  ACMYQ1_RS15550 (ACMYQ1_15550) hemH 3589506..3590519 (-) 1014 WP_393942004.1 ferrochelatase -
  ACMYQ1_RS15555 (ACMYQ1_15555) - 3590552..3591091 (-) 540 WP_393942006.1 glutathione peroxidase -
  ACMYQ1_RS15560 (ACMYQ1_15560) pilU 3591278..3592390 (-) 1113 WP_028761282.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACMYQ1_RS15565 (ACMYQ1_15565) pilT 3592400..3593437 (-) 1038 WP_006082517.1 type IV pilus twitching motility protein PilT Machinery gene
  ACMYQ1_RS15570 (ACMYQ1_15570) - 3593473..3594171 (+) 699 WP_415775122.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACMYQ1_RS15575 (ACMYQ1_15575) proC 3594388..3595206 (+) 819 WP_393942011.1 pyrroline-5-carboxylate reductase -
  ACMYQ1_RS15580 (ACMYQ1_15580) - 3595253..3595801 (+) 549 WP_393942013.1 YggT family protein -
  ACMYQ1_RS15585 (ACMYQ1_15585) yggU 3595801..3596106 (+) 306 WP_219253476.1 DUF167 family protein YggU -
  ACMYQ1_RS15590 (ACMYQ1_15590) - 3596242..3596676 (+) 435 WP_113939419.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41029.16 Da        Isoelectric Point: 6.3720

>NTDB_id=1096015 ACMYQ1_RS15560 WP_028761282.1 3591278..3592390(-) (pilU) [Shewanella oncorhynchi strain XWfb1]
MDVRPFLKVIVERKASDLFITAGFPPSAKIDGELRPLAESAFTPAQSLDFVESLMTEAQKQEFHSTRECNFAFAAKDLGR
FRVSAFWQRESPGCVMRRIETKIPDVDDLKLPPILKDLVMSKRGLIIMVGGTGTGKSTSLAALVGYRNGHARGHILTIED
PVEFVHDHRKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLIPKVDGTGRRAAIEVLINTPRVASLIAKNELHLLKETMAKSNEQGMQTFDQA
LLKLYVDGEISYADALHHADSPNDLRLMIKLQNKESTGSGFMEGVTLDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=1096015 ACMYQ1_RS15560 WP_028761282.1 3591278..3592390(-) (pilU) [Shewanella oncorhynchi strain XWfb1]
ATGGATGTTCGTCCGTTTTTAAAAGTGATAGTGGAACGCAAAGCCTCCGACTTGTTCATCACTGCGGGTTTTCCGCCGAG
TGCCAAGATTGACGGTGAATTGCGTCCCTTGGCAGAAAGTGCGTTTACACCAGCACAATCCCTGGATTTTGTTGAGTCGT
TAATGACTGAGGCTCAGAAGCAAGAGTTTCATTCCACTCGTGAGTGTAACTTCGCTTTTGCGGCAAAGGATTTAGGTCGT
TTTCGGGTGAGCGCTTTTTGGCAGCGTGAATCGCCTGGCTGCGTGATGCGCCGTATTGAGACTAAAATCCCTGATGTCGA
TGATTTAAAATTGCCGCCTATTCTAAAAGATCTGGTGATGAGCAAACGTGGCCTCATCATTATGGTAGGCGGAACGGGAA
CGGGTAAGTCAACGTCTTTGGCGGCCTTGGTGGGTTATCGCAATGGTCATGCTCGTGGGCATATTTTGACTATTGAAGAT
CCGGTTGAATTCGTTCATGACCACAGAAAAAGCATAATCACTCAGCGAGAAGTGGGTATAGATACTGAGTCTTTTGATGC
GGCGCTAAAAAGCTCCCTTCGCCAAGCTCCCGATGTGATCTTGATTGGTGAAATTCGTACCCAAGAGACGATGGAGTTTG
CGCTGTCATTTGCAGAAACGGGTCACCTTTGTATGGCAACCTTGCATGCCAACAACGCCAACCAAGCGTTAGACCGGATT
ATGCATCTCGTGCCTGAGAGTAAGCATCAACAGTTATTGTTCGATTTATCATTGAATTTACGCGGTATTGTGGCGCAGCA
ATTGATACCGAAAGTCGATGGCACAGGGCGTCGCGCCGCGATTGAAGTATTGATTAACACCCCAAGGGTGGCGAGTTTAA
TTGCGAAGAACGAGCTGCATTTGCTCAAAGAAACCATGGCAAAATCCAACGAGCAGGGCATGCAGACGTTCGATCAGGCC
TTGCTGAAACTTTATGTCGATGGCGAGATTAGTTACGCCGATGCGCTGCACCATGCTGATTCACCAAACGATTTACGTTT
GATGATTAAATTGCAGAATAAAGAATCCACGGGCTCAGGTTTTATGGAAGGTGTCACCTTAGACATGGACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.946

100

0.603
  pilU Vibrio cholerae strain A1552

58.919

100

0.589
  pilU Acinetobacter baylyi ADP1

61.08

95.135

0.581
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.556

97.297

0.395
  pilT Vibrio cholerae strain A1552

42.424

89.189

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.424

89.189

0.378
  pilT Acinetobacter baumannii D1279779

39.181

92.432

0.362
  pilT Acinetobacter baumannii strain A118

39.181

92.432

0.362
  pilT Acinetobacter nosocomialis M2

39.181

92.432

0.362
  pilT Legionella pneumophila strain Lp02

40.483

89.459

0.362
  pilT Legionella pneumophila strain ERS1305867

40.483

89.459

0.362

Multiple sequence alignment