Detailed information NTG1095993

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: ACMYQ0_RS15645
• Genome accession: NZ_CP180477
• Coordinates: 3384032..3385066 (-)
• Length: 344 a.a.
• NCBI ID: WP_415753712.1
• Uniprot ID: -
• Organism: Pseudomonas leptonychotis strain CCM 8849
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3379032..3390066

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACMYQ0_RS15625	-	3380168..3380566 (-)	399	WP_415753708.1	NINE protein	-
ACMYQ0_RS15630	-	3380725..3381936 (+)	1212	WP_415753709.1	putative DNA modification/repair radical SAM protein	-
ACMYQ0_RS15635	-	3381936..3382814 (+)	879	WP_415753710.1	TIGR03915 family putative DNA repair protein	-
ACMYQ0_RS15640	pilU	3382780..3383925 (-)	1146	WP_415753711.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACMYQ0_RS15645	pilT	3384032..3385066 (-)	1035	WP_415753712.1	type IV pilus twitching motility protein PilT	Machinery gene
ACMYQ0_RS15650	-	3385130..3385816 (+)	687	WP_136663429.1	YggS family pyridoxal phosphate-dependent enzyme	-
ACMYQ0_RS15655	proC	3385845..3386663 (+)	819	WP_136663430.1	pyrroline-5-carboxylate reductase	-
ACMYQ0_RS15660	-	3386673..3387263 (+)	591	WP_415753713.1	YggT family protein	-
ACMYQ0_RS15665	-	3387609..3389591 (+)	1983	WP_415753714.1	dynamin family protein	-

Sequence

Protein

• Length: 344 a.a.
• Molecular weight: 37897.67 Da
• Isoelectric Point: 6.8361

>NTDB_id=1095993 ACMYQ0_RS15645 WP_415753712.1 3384032..3385066(-) (pilT) [Pseudomonas leptonychotis strain CCM 8849]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEDLGMGEIFKKISDVPRGLVLVTGPTGSGKSTTLAAMLDYINSNKYHHILTVED
PIEFVHESKKCLINQREVHRDTLGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQSVISQTLLKKVGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGALGMQTLDSCL
KTLLSKGIVSREAAREKAKIPENF

Nucleotide

• Length: 1035 bp

>NTDB_id=1095993 ACMYQ0_RS15645 WP_415753712.1 3384032..3385066(-) (pilT) [Pseudomonas leptonychotis strain CCM 8849]
ATGGATATCACTGAATTGCTGGCCTTCAGCGCCAAGCAAGGTGCATCGGACCTGCACCTCTCCGCTGGCCTGCCTCCGAT
GATTCGGGTCGACGGTGATGTTCGCCGGATCAATTTGCCTGCACTCGACCATAAACAAGTGCACGCGCTGATCTACGACA
TCATGAACGATAAGCAGCGTAAGGATTTCGAGGAGTTCCTCGAGACCGACTTCTCCTTCGAAGTGCCGGGTGTAGCGCGT
TTCCGGGTTAACGCCTTCAACCAGAACCGTGGCGCTGGCGCCGTATTCCGTACCATTCCGTCGAAAGTGCTGAGCATGGA
AGATCTCGGTATGGGTGAGATCTTCAAGAAGATTTCTGATGTGCCGCGGGGCTTGGTGCTGGTCACCGGGCCCACCGGTT
CGGGCAAATCGACCACTTTGGCGGCGATGCTCGACTATATAAATAGCAACAAGTACCACCACATCCTCACGGTGGAAGAC
CCGATCGAGTTCGTTCACGAATCGAAAAAATGCCTGATTAACCAGCGCGAAGTGCACCGCGACACCCTCGGCTTCTCCGA
AGCCTTGCGCTCGGCGCTGCGTGAAGACCCGGATATCATCCTGGTGGGCGAAATGCGCGACCTGGAAACCATTCGCCTGG
CGCTGACTGCCGCGGAAACGGGTCACCTGGTATTTGGCACTCTGCACACCACCTCAGCGGCTAAAACCATCGACCGGGTG
GTCGACGTATTCCCCGCCGAAGAGAAATCCATGGTGCGTTCCATGCTCTCGGAGTCCTTGCAGTCGGTGATCTCGCAAAC
CCTGTTGAAAAAGGTTGGCGGCGGCCGTGTGGCGGCACACGAGATCATGATTGGTACGCCGGCTATCCGTAACCTGATCC
GTGAGGACAAGGTGGCGCAGATGTATTCGGCCATCCAGACCGGTGGCGCGTTGGGCATGCAAACCCTCGATTCCTGCCTG
AAGACCTTGTTGTCCAAAGGCATCGTCAGCCGCGAAGCCGCGCGGGAAAAAGCCAAAATTCCGGAAAACTTCTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (11-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Pseudomonas stutzeri DSM 10701	93.605	100	0.936
pilT	Pseudomonas aeruginosa PAK	93.314	100	0.933
pilT	Acinetobacter nosocomialis M2	85.465	100	0.855
pilT	Acinetobacter baumannii D1279779	85.174	100	0.852
pilT	Acinetobacter baumannii strain A118	85.174	100	0.852
pilT	Acinetobacter baylyi ADP1	83.14	100	0.831
pilT	Legionella pneumophila strain Lp02	76.036	98.256	0.747
pilT	Legionella pneumophila strain ERS1305867	76.036	98.256	0.747
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	68.529	98.837	0.677
pilT	Vibrio cholerae strain A1552	68.529	98.837	0.677
pilT	Neisseria gonorrhoeae MS11	67.246	100	0.674
pilT	Neisseria meningitidis 8013	67.246	100	0.674
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	51.304	100	0.515
pilU	Acinetobacter baylyi ADP1	39.884	100	0.401
pilU	Vibrio cholerae strain A1552	40.413	98.547	0.398
pilU	Pseudomonas stutzeri DSM 10701	40	97.384	0.39