Detailed information NTG1095992

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACMYQ0_RS15640
• Genome accession: NZ_CP180477
• Coordinates: 3382780..3383925 (-)
• Length: 381 a.a.
• NCBI ID: WP_415753711.1
• Uniprot ID: -
• Organism: Pseudomonas leptonychotis strain CCM 8849
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3377780..3388925

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACMYQ0_RS15615	-	3377833..3378843 (+)	1011	WP_136663422.1	aspartate carbamoyltransferase catalytic subunit	-
ACMYQ0_RS15620	-	3378843..3380114 (+)	1272	WP_415753707.1	dihydroorotase	-
ACMYQ0_RS15625	-	3380168..3380566 (-)	399	WP_415753708.1	NINE protein	-
ACMYQ0_RS15630	-	3380725..3381936 (+)	1212	WP_415753709.1	putative DNA modification/repair radical SAM protein	-
ACMYQ0_RS15635	-	3381936..3382814 (+)	879	WP_415753710.1	TIGR03915 family putative DNA repair protein	-
ACMYQ0_RS15640	pilU	3382780..3383925 (-)	1146	WP_415753711.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACMYQ0_RS15645	pilT	3384032..3385066 (-)	1035	WP_415753712.1	type IV pilus twitching motility protein PilT	Machinery gene
ACMYQ0_RS15650	-	3385130..3385816 (+)	687	WP_136663429.1	YggS family pyridoxal phosphate-dependent enzyme	-
ACMYQ0_RS15655	proC	3385845..3386663 (+)	819	WP_136663430.1	pyrroline-5-carboxylate reductase	-
ACMYQ0_RS15660	-	3386673..3387263 (+)	591	WP_415753713.1	YggT family protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42548.73 Da
• Isoelectric Point: 6.6646

>NTDB_id=1095992 ACMYQ0_RS15640 WP_415753711.1 3382780..3383925(-) (pilU) [Pseudomonas leptonychotis strain CCM 8849]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPITKTPMSPEQTRETVHSVMNEQQRRDFTENHECNFAISARGVGR
FRVSAFYQRNLAGMVLRRIETNIPTLDELKLPEILKKLALTKRGLVLFVGATGTGKSTSLAAMIGYRNKNSSGHIISIED
PIEYIHQHQSCIVTQREVGIDTESFEVALKNTLRQAPDVILIGEVRTRETMDHAVAFAETGHLCLATLHANNANQALDRI
INFFPADRQNQVWMDLSLNLKAIVAQQLIPTPDGKGRRAVIEVLINTPLASDLIRKGDVHELKALMKRSTEQGMQTFDQA
LYNLYSQGEITYEDALLYADSANDLRLMIKLGSESDGDHLSSMAQGLSLEVSEEDPGRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=1095992 ACMYQ0_RS15640 WP_415753711.1 3382780..3383925(-) (pilU) [Pseudomonas leptonychotis strain CCM 8849]
ATGGAATTCGAAAAACTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCTGACCTGTTTATCACTGCCGGTGTACCGCCGTC
GATGAAGGTCAATGGCAAGATCATGCCAATCACCAAGACGCCCATGTCGCCTGAGCAGACGCGTGAAACTGTGCACTCGG
TGATGAATGAGCAGCAGCGCCGCGATTTCACGGAGAACCATGAGTGCAATTTCGCGATCAGCGCCCGCGGTGTGGGTCGT
TTCCGCGTCAGTGCCTTTTACCAGCGCAACTTGGCCGGTATGGTGCTGCGCCGCATCGAGACCAATATCCCGACGTTGGA
CGAGCTGAAACTGCCGGAAATTCTCAAGAAACTGGCGCTGACCAAGCGCGGCCTGGTGTTGTTCGTGGGAGCCACCGGCA
CGGGTAAGTCGACCTCGCTGGCAGCAATGATTGGCTACCGCAACAAGAACAGCAGCGGTCATATCATTTCCATCGAAGAC
CCGATCGAATACATCCACCAGCACCAGAGCTGCATCGTCACTCAGCGTGAGGTGGGCATCGACACCGAGTCGTTCGAAGT
AGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATTTTGATTGGTGAGGTGCGCACCCGCGAGACCATGGACCACG
CCGTGGCGTTCGCTGAAACCGGCCACTTATGCCTGGCCACGCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATT
ATCAACTTCTTCCCGGCGGACCGGCAAAACCAGGTGTGGATGGATCTGTCGCTCAACCTCAAAGCCATCGTTGCTCAGCA
ACTGATCCCAACGCCGGATGGTAAGGGCCGCCGTGCGGTGATTGAGGTGCTGATCAATACGCCACTGGCTTCCGATCTGA
TCCGTAAGGGTGATGTGCATGAGCTCAAAGCGTTGATGAAACGCTCCACCGAGCAGGGCATGCAGACCTTCGATCAGGCG
CTGTACAACCTATACAGCCAAGGCGAGATTACCTACGAAGACGCGTTGCTTTACGCTGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTGGGTTCTGAAAGTGACGGTGATCACTTGAGCAGCATGGCTCAGGGGCTGTCGCTGGAAGTCAGTGAGG
AAGATCCGGGTCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	91.076	100	0.911
pilU	Acinetobacter baylyi ADP1	64.426	93.701	0.604
pilU	Vibrio cholerae strain A1552	58.571	91.864	0.538
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.75	88.189	0.386
pilT	Neisseria gonorrhoeae MS11	41.791	87.927	0.367
pilT	Neisseria meningitidis 8013	41.791	87.927	0.367
pilT	Pseudomonas aeruginosa PAK	41.493	87.927	0.365