Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACMXD4_RS00945 Genome accession   NZ_CP180476
Coordinates   209997..211034 (+) Length   345 a.a.
NCBI ID   WP_101053853.1    Uniprot ID   A0A2N0V4Z8
Organism   Methylomonas sp. TEB     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 204997..216034
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMXD4_RS00920 (ACMXD4_00920) - 205214..205477 (+) 264 WP_171696921.1 hypothetical protein -
  ACMXD4_RS00925 (ACMXD4_00925) - 205589..207538 (-) 1950 WP_415879590.1 transglutaminase TgpA family protein -
  ACMXD4_RS00930 (ACMXD4_00930) - 207541..208491 (-) 951 WP_415879591.1 DUF58 domain-containing protein -
  ACMXD4_RS00935 (ACMXD4_00935) - 208488..209393 (-) 906 WP_415879592.1 AAA family ATPase -
  ACMXD4_RS00940 (ACMXD4_00940) - 209539..209880 (+) 342 WP_415879593.1 TraR/DksA family transcriptional regulator -
  ACMXD4_RS00945 (ACMXD4_00945) pilT 209997..211034 (+) 1038 WP_101053853.1 type IV pilus twitching motility protein PilT Machinery gene
  ACMXD4_RS00950 (ACMXD4_00950) pilU 211050..212183 (+) 1134 WP_033158298.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACMXD4_RS00955 (ACMXD4_00955) mltB 212201..213382 (+) 1182 WP_033158297.1 lytic murein transglycosylase B -
  ACMXD4_RS00960 (ACMXD4_00960) - 213883..214437 (-) 555 WP_129871319.1 hypothetical protein -
  ACMXD4_RS00965 (ACMXD4_00965) - 214653..215189 (-) 537 WP_033158295.1 REP-associated tyrosine transposase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38214.12 Da        Isoelectric Point: 7.0291

>NTDB_id=1095949 ACMXD4_RS00945 WP_101053853.1 209997..211034(+) (pilT) [Methylomonas sp. TEB]
MDIAELLTFSVKNKASDLHLSAGLPPMIRVDGDIRRINIPSLNHKEVHALIYDIMNDKQRRDYEEFLETDFSFALPGVAR
FRVNAFNQDRGAGAVFRTIPSKVLTLEDLDAPKFFAELCTKPRGLILVTGPTGSGKSTTLAAMVNHINTNDYAHILTVED
PIEFVHESQKCLINQREVHRDTLGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPAAEKDMIRAMLSESLQAVISQTLLKKVGGGRIAAHEIMVGTPAIRNLIREAKVAQMYSAIQTGRKDGMQTLDQNL
KELVDKGLITAKGAMVKAVNKDMFR

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1095949 ACMXD4_RS00945 WP_101053853.1 209997..211034(+) (pilT) [Methylomonas sp. TEB]
ATGGATATCGCCGAACTATTGACCTTTTCCGTCAAAAACAAAGCCTCCGACTTGCATCTTTCCGCCGGCTTACCGCCGAT
GATCCGGGTGGACGGCGATATCCGCCGCATCAATATCCCGTCCTTGAATCACAAGGAAGTCCACGCGCTGATTTACGACA
TCATGAACGACAAGCAGCGCCGCGATTACGAAGAATTTCTGGAAACCGACTTTTCTTTCGCGTTGCCCGGCGTGGCGCGC
TTCCGGGTGAATGCCTTCAATCAGGATAGAGGCGCCGGCGCGGTATTCCGGACCATTCCGTCCAAGGTTTTGACCCTGGA
AGACCTGGACGCGCCGAAATTTTTCGCGGAACTGTGTACCAAGCCGCGCGGCCTGATTCTGGTGACCGGTCCGACCGGTT
CCGGTAAATCCACCACGCTGGCGGCGATGGTGAATCACATTAATACCAACGATTACGCACATATCCTGACCGTCGAAGAC
CCTATCGAGTTTGTCCACGAAAGCCAGAAATGCTTGATCAACCAGCGCGAAGTACATCGCGATACCTTAGGTTTCAACGA
AGCCTTGCGCTCGGCATTACGGGAAGACCCGGACATCATTCTGGTGGGTGAGATGCGGGATTTGGAAACCATCCGCCTTG
CCTTGACCGCCGCGGAAACCGGCCACTTGGTGTTCGGCACCCTGCATACCACCTCGGCCGCGAAAACCATCGACCGGATT
ATCGACGTGTTCCCTGCCGCCGAAAAAGATATGATCCGGGCCATGCTGTCCGAATCCTTGCAGGCGGTTATCTCGCAAAC
GCTATTGAAAAAAGTCGGTGGCGGCCGGATTGCCGCCCACGAAATCATGGTCGGCACCCCTGCCATCCGCAACCTGATCC
GCGAAGCCAAGGTGGCGCAGATGTATTCCGCGATCCAAACCGGCCGTAAAGACGGCATGCAGACGCTGGATCAAAACCTG
AAGGAATTGGTCGACAAAGGCTTGATTACTGCGAAAGGCGCGATGGTCAAAGCCGTCAACAAAGATATGTTCCGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2N0V4Z8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

80.233

99.71

0.8
  pilT Acinetobacter baumannii strain A118

80.233

99.71

0.8
  pilT Acinetobacter nosocomialis M2

80.233

99.71

0.8
  pilT Acinetobacter baylyi ADP1

79.36

99.71

0.791
  pilT Pseudomonas stutzeri DSM 10701

79.36

99.71

0.791
  pilT Pseudomonas aeruginosa PAK

80.473

97.971

0.788
  pilT Legionella pneumophila strain Lp02

72.965

99.71

0.728
  pilT Legionella pneumophila strain ERS1305867

72.965

99.71

0.728
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.03

97.681

0.684
  pilT Vibrio cholerae strain A1552

70.03

97.681

0.684
  pilT Neisseria meningitidis 8013

66.181

99.42

0.658
  pilT Neisseria gonorrhoeae MS11

65.889

99.42

0.655
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

100

0.507
  pilU Acinetobacter baylyi ADP1

40.634

100

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.173

100

0.403
  pilU Vibrio cholerae strain A1552

40.118

98.261

0.394

Multiple sequence alignment