Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACLM45_RS09240 Genome accession   NZ_CP180406
Coordinates   1686889..1687962 (+) Length   357 a.a.
NCBI ID   WP_415408919.1    Uniprot ID   -
Organism   Synechococcus sp. A10-1-5-9     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1681889..1692962
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLM45_RS09215 (ACLM45_09215) rsgA 1682084..1682965 (-) 882 WP_415411398.1 ribosome small subunit-dependent GTPase A -
  ACLM45_RS09220 (ACLM45_09220) - 1682985..1683227 (-) 243 WP_006042665.1 sulfurtransferase TusA family protein -
  ACLM45_RS09225 (ACLM45_09225) dnaJ 1683230..1684360 (-) 1131 WP_006042666.1 molecular chaperone DnaJ -
  ACLM45_RS09230 (ACLM45_09230) grpE 1684411..1685124 (-) 714 WP_415408915.1 nucleotide exchange factor GrpE -
  ACLM45_RS09235 (ACLM45_09235) pilB/pilB1 1685220..1686878 (+) 1659 WP_415408917.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  ACLM45_RS09240 (ACLM45_09240) pilT 1686889..1687962 (+) 1074 WP_415408919.1 type IV pilus twitching motility protein PilT Machinery gene
  ACLM45_RS09245 (ACLM45_09245) - 1687979..1689232 (+) 1254 WP_415408921.1 type II secretion system F family protein -
  ACLM45_RS09250 (ACLM45_09250) - 1689267..1690529 (-) 1263 WP_415411399.1 NAD(P)/FAD-dependent oxidoreductase -
  ACLM45_RS09255 (ACLM45_09255) msrB 1690501..1691019 (-) 519 WP_415408923.1 peptide-methionine (R)-S-oxide reductase MsrB -
  ACLM45_RS09260 (ACLM45_09260) dusA 1691081..1692085 (+) 1005 WP_415408925.1 tRNA dihydrouridine(20/20a) synthase DusA -
  ACLM45_RS09265 (ACLM45_09265) - 1692104..1692685 (-) 582 WP_415408927.1 RNA recognition motif domain-containing protein -

Sequence

Protein


Download         Length: 357 a.a.        Molecular weight: 39270.93 Da        Isoelectric Point: 6.3944

>NTDB_id=1095721 ACLM45_RS09240 WP_415408919.1 1686889..1687962(+) (pilT) [Synechococcus sp. A10-1-5-9]
MELMIEDLMQELVEAGGSDLHIASGQPPYGRFSGELRPMRDDPLMEESCNRLIFSMLNNSQRKTLEQTWELDCAYGLKGV
ARFRVNVYRQKGSYAACLRALGSKIPSIELLNLPPVVVETSKRPRGLVLVTGPTGSGKTTTLAALLDHINHSRAEHILTI
EDPIEFVYKSDQSLVHQRQLNEDTRSFANALRAALREDPDVILVGEMRDLETIQLAISAAETGHLVFGTLHTSSAAQTVD
RMVDVFPPAQQTQIRVQLSGSLVAVFSQTLCRRRNPAPGQFGRVMAQEIMINTPAIANLIREGKTAQLYSQIQTGGELGM
QTLEKALADLINRGDITLQEARAKASKPSELDRLAAG

Nucleotide


Download         Length: 1074 bp        

>NTDB_id=1095721 ACLM45_RS09240 WP_415408919.1 1686889..1687962(+) (pilT) [Synechococcus sp. A10-1-5-9]
ATGGAACTGATGATCGAGGACCTGATGCAGGAGCTGGTGGAGGCAGGCGGCAGCGACCTTCACATCGCCAGCGGTCAACC
CCCCTACGGACGATTTAGCGGTGAGCTACGACCCATGCGGGACGATCCCCTGATGGAGGAAAGCTGCAACCGGCTGATCT
TCTCCATGCTTAACAACAGCCAGCGCAAAACACTCGAACAGACCTGGGAGCTCGACTGTGCCTACGGACTCAAAGGCGTG
GCCCGCTTCCGGGTGAATGTGTACCGCCAGAAAGGCAGCTATGCCGCCTGTCTGCGCGCTCTCGGTAGCAAAATCCCCAG
CATCGAGCTGCTGAATCTTCCTCCCGTTGTGGTGGAAACGAGCAAACGCCCCCGGGGATTAGTGCTCGTGACCGGCCCGA
CTGGGTCAGGCAAAACCACCACCCTGGCGGCCCTGCTGGATCACATCAACCACAGCCGGGCGGAACACATCCTCACAATC
GAGGATCCGATCGAATTCGTGTACAAGAGCGATCAGAGCCTGGTGCATCAACGCCAGCTCAACGAAGACACCCGCAGTTT
CGCCAATGCACTGCGAGCAGCCCTGAGGGAAGACCCCGACGTCATCCTGGTGGGAGAAATGCGCGATCTGGAAACCATCC
AGCTGGCGATCAGCGCTGCTGAAACCGGGCACCTGGTGTTCGGCACGTTGCACACCAGCTCAGCGGCCCAGACCGTGGAC
CGCATGGTGGATGTGTTCCCTCCCGCTCAGCAGACCCAAATCCGCGTGCAGCTCTCGGGGAGCCTGGTAGCCGTGTTCTC
TCAAACCCTATGCCGCCGCCGCAACCCGGCACCTGGACAATTCGGCCGGGTGATGGCGCAGGAAATCATGATCAACACGC
CGGCCATCGCCAATCTCATCCGCGAAGGAAAAACCGCCCAGCTGTATTCACAGATTCAAACCGGTGGCGAACTGGGCATG
CAGACCCTGGAGAAGGCGCTAGCAGATCTGATCAATCGAGGAGACATCACCCTTCAGGAGGCGCGGGCAAAGGCGAGCAA
ACCCTCCGAACTGGATCGGCTCGCGGCTGGTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(121-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

53.314

97.199

0.518
  pilT Pseudomonas stutzeri DSM 10701

52.738

97.199

0.513
  pilT Acinetobacter baylyi ADP1

51.724

97.479

0.504
  pilT Acinetobacter baumannii D1279779

51.585

97.199

0.501
  pilT Acinetobacter baumannii strain A118

51.585

97.199

0.501
  pilT Acinetobacter nosocomialis M2

51.585

97.199

0.501
  pilT Legionella pneumophila strain Lp02

51.884

96.639

0.501
  pilT Legionella pneumophila strain ERS1305867

51.884

96.639

0.501
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.573

98.319

0.487
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.588

95.238

0.482
  pilT Vibrio cholerae strain A1552

50.588

95.238

0.482
  pilT Neisseria meningitidis 8013

49.854

96.078

0.479
  pilT Neisseria gonorrhoeae MS11

49.563

96.078

0.476
  pilU Pseudomonas stutzeri DSM 10701

39.193

97.199

0.381
  pilU Acinetobacter baylyi ADP1

37.356

97.479

0.364
  pilU Vibrio cholerae strain A1552

38.507

93.838

0.361

Multiple sequence alignment