Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACLBLI_RS14395 Genome accession   NZ_CP180210
Coordinates   3288083..3289120 (-) Length   345 a.a.
NCBI ID   WP_414828284.1    Uniprot ID   -
Organism   Alteromonas sp. H39     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3283083..3294120
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLBLI_RS14360 (ACLBLI_14360) - 3283115..3283291 (+) 177 WP_414828277.1 hypothetical protein -
  ACLBLI_RS14365 (ACLBLI_14365) - 3283461..3284189 (+) 729 WP_414828278.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACLBLI_RS14370 (ACLBLI_14370) gshB 3284560..3285510 (+) 951 WP_414828279.1 glutathione synthase -
  ACLBLI_RS14375 (ACLBLI_14375) - 3285538..3286104 (+) 567 WP_414828280.1 YqgE/AlgH family protein -
  ACLBLI_RS14380 (ACLBLI_14380) ruvX 3286097..3286528 (+) 432 WP_414828281.1 Holliday junction resolvase RuvX -
  ACLBLI_RS14385 (ACLBLI_14385) - 3286605..3286952 (-) 348 WP_414828282.1 DUF2007 domain-containing protein -
  ACLBLI_RS14390 (ACLBLI_14390) pilU 3286964..3288073 (-) 1110 WP_414828283.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACLBLI_RS14395 (ACLBLI_14395) pilT 3288083..3289120 (-) 1038 WP_414828284.1 type IV pilus twitching motility protein PilT Machinery gene
  ACLBLI_RS14400 (ACLBLI_14400) - 3289150..3289836 (+) 687 WP_414828285.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACLBLI_RS14405 (ACLBLI_14405) proC 3289885..3290706 (+) 822 WP_414828286.1 pyrroline-5-carboxylate reductase -
  ACLBLI_RS14410 (ACLBLI_14410) - 3290727..3291266 (+) 540 WP_414828287.1 YggT family protein -
  ACLBLI_RS14415 (ACLBLI_14415) - 3291288..3291713 (+) 426 WP_414828288.1 DUF4426 domain-containing protein -
  ACLBLI_RS14420 (ACLBLI_14420) - 3291829..3292740 (-) 912 WP_414828289.1 hypothetical protein -
  ACLBLI_RS14425 (ACLBLI_14425) - 3292916..3293251 (-) 336 WP_414828290.1 VOC family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38287.01 Da        Isoelectric Point: 6.6953

>NTDB_id=1094567 ACLBLI_RS14395 WP_414828284.1 3288083..3289120(-) (pilT) [Alteromonas sp. H39]
MDITELLAFSVKNNASDLHLTAGLPPIIRVDGEMRKLNVPALDHKQVHALVYEIMNDKQRKEYEENLEVDFSFEVKDLSR
FRVNAFLQNRGAAAVLRTIPSNVLTLDELGAPQIFKDIINQPTGIVLVTGATGSGKSTTLAAMVDHINTHKREHILTIED
PIEFVHDNKLSVINQREVHRDTHSFSNALRSALREDPDVILVGELRDLETIRLAISAAETGHLVFGTLHTNSAPKTIDRI
IDVFPAEEKSMVRSMLSESLRAVISQTLLKKIGGGRVAAHEIMVGIPAIRNLIREDKVPQMYSVIQTGQSHGMQTMDQCL
QRLVAMGVITQQDAAAKAIDKKTNF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1094567 ACLBLI_RS14395 WP_414828284.1 3288083..3289120(-) (pilT) [Alteromonas sp. H39]
GTGGATATTACCGAACTTTTGGCTTTCAGTGTCAAAAATAACGCCTCAGACTTACACCTGACAGCAGGCTTACCGCCGAT
AATCAGGGTTGATGGCGAAATGCGAAAGCTAAACGTTCCTGCGCTGGATCATAAACAGGTACACGCATTGGTGTATGAGA
TTATGAACGACAAGCAGCGCAAGGAATACGAGGAAAACCTTGAGGTGGATTTTTCCTTTGAAGTGAAAGATCTCTCCCGC
TTCCGTGTGAATGCCTTCCTGCAAAACCGCGGTGCCGCGGCAGTATTGCGTACCATCCCCAGCAATGTACTGACGCTGGA
TGAACTGGGCGCGCCGCAGATTTTCAAAGATATTATTAATCAGCCCACCGGGATTGTTCTGGTGACGGGGGCTACCGGTT
CGGGTAAAAGTACCACGCTGGCTGCCATGGTCGATCATATCAATACGCATAAGCGCGAACATATTCTGACCATCGAAGAT
CCTATCGAATTCGTACATGACAACAAGCTCAGTGTTATTAACCAGCGTGAAGTACATCGTGATACACACAGCTTCTCTAA
TGCCCTGCGTTCAGCACTGCGTGAAGATCCTGACGTGATCCTGGTGGGTGAGCTACGTGACCTGGAAACCATTCGACTGG
CAATCTCAGCGGCAGAAACCGGCCACCTGGTTTTCGGTACCCTGCACACTAACTCAGCGCCGAAAACCATAGACCGGATC
ATCGATGTGTTTCCCGCGGAAGAGAAGTCGATGGTGCGCTCTATGCTGTCGGAATCCTTACGTGCGGTAATTTCTCAAAC
GTTACTTAAAAAAATTGGTGGCGGGCGCGTGGCAGCACACGAGATCATGGTGGGTATTCCGGCCATCCGAAACCTTATTC
GTGAAGATAAAGTCCCGCAAATGTACTCTGTCATTCAAACTGGACAATCCCACGGTATGCAGACCATGGATCAGTGTTTG
CAGCGCCTTGTTGCCATGGGCGTGATTACTCAGCAGGATGCAGCGGCAAAGGCTATCGATAAGAAAACGAATTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

76.627

97.971

0.751
  pilT Acinetobacter baumannii strain A118

76.627

97.971

0.751
  pilT Acinetobacter nosocomialis M2

76.331

97.971

0.748
  pilT Acinetobacter baylyi ADP1

75.148

97.971

0.736
  pilT Pseudomonas aeruginosa PAK

74.556

97.971

0.73
  pilT Pseudomonas stutzeri DSM 10701

74.556

97.971

0.73
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.81

97.681

0.701
  pilT Vibrio cholerae strain A1552

71.81

97.681

0.701
  pilT Legionella pneumophila strain Lp02

69.795

98.841

0.69
  pilT Legionella pneumophila strain ERS1305867

69.795

98.841

0.69
  pilT Neisseria meningitidis 8013

65.116

99.71

0.649
  pilT Neisseria gonorrhoeae MS11

64.826

99.71

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

54.227

99.42

0.539
  pilU Vibrio cholerae strain A1552

42.388

97.101

0.412
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.101

0.403
  pilU Acinetobacter baylyi ADP1

40.882

98.551

0.403

Multiple sequence alignment