Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACLBLI_RS14390 Genome accession   NZ_CP180210
Coordinates   3286964..3288073 (-) Length   369 a.a.
NCBI ID   WP_414828283.1    Uniprot ID   -
Organism   Alteromonas sp. H39     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3281964..3293073
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLBLI_RS14360 (ACLBLI_14360) - 3283115..3283291 (+) 177 WP_414828277.1 hypothetical protein -
  ACLBLI_RS14365 (ACLBLI_14365) - 3283461..3284189 (+) 729 WP_414828278.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACLBLI_RS14370 (ACLBLI_14370) gshB 3284560..3285510 (+) 951 WP_414828279.1 glutathione synthase -
  ACLBLI_RS14375 (ACLBLI_14375) - 3285538..3286104 (+) 567 WP_414828280.1 YqgE/AlgH family protein -
  ACLBLI_RS14380 (ACLBLI_14380) ruvX 3286097..3286528 (+) 432 WP_414828281.1 Holliday junction resolvase RuvX -
  ACLBLI_RS14385 (ACLBLI_14385) - 3286605..3286952 (-) 348 WP_414828282.1 DUF2007 domain-containing protein -
  ACLBLI_RS14390 (ACLBLI_14390) pilU 3286964..3288073 (-) 1110 WP_414828283.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACLBLI_RS14395 (ACLBLI_14395) pilT 3288083..3289120 (-) 1038 WP_414828284.1 type IV pilus twitching motility protein PilT Machinery gene
  ACLBLI_RS14400 (ACLBLI_14400) - 3289150..3289836 (+) 687 WP_414828285.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACLBLI_RS14405 (ACLBLI_14405) proC 3289885..3290706 (+) 822 WP_414828286.1 pyrroline-5-carboxylate reductase -
  ACLBLI_RS14410 (ACLBLI_14410) - 3290727..3291266 (+) 540 WP_414828287.1 YggT family protein -
  ACLBLI_RS14415 (ACLBLI_14415) - 3291288..3291713 (+) 426 WP_414828288.1 DUF4426 domain-containing protein -
  ACLBLI_RS14420 (ACLBLI_14420) - 3291829..3292740 (-) 912 WP_414828289.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40928.80 Da        Isoelectric Point: 5.7314

>NTDB_id=1094566 ACLBLI_RS14390 WP_414828283.1 3286964..3288073(-) (pilU) [Alteromonas sp. H39]
MLDTFLEQMVSQSASDLFVTAGFPVSAKINGKLTPLTDMPLSEEEALSLVHESMNEKQQNEFHNSRECNFAIARDGVGRF
RCSAFWQRDQAGMVIRRIVTQIPRVDDLGLPPVLKDIIMSKRGLVLFVGGTGTGKSTSLAALIGHRNQNSHGHILTIEDP
IEFVHEHRNCVITQREVGLDTQTFDDALKSSLRQAPDVILIGEIRSMETMEYAMSFADTGHLCVATLHANNANQAIERIM
HLAPKDQHDKLRFDLSLNIRAIVAQQLVPTRDGKGRVAAIEILLNSPLVTDLIQRNEIGSLKEAMKKGKEMGMQSFDMAL
YDLYKAGKIELDQALHHADSPNDLRLMIKLDTNEGSSLGTLSDVSIDMD

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=1094566 ACLBLI_RS14390 WP_414828283.1 3286964..3288073(-) (pilU) [Alteromonas sp. H39]
ATGCTAGATACATTTTTAGAACAGATGGTGTCGCAATCCGCGTCTGATTTATTTGTGACTGCCGGCTTCCCTGTCAGCGC
CAAGATTAACGGTAAGCTGACTCCTCTGACAGATATGCCTCTCTCGGAAGAAGAAGCGTTGTCGCTGGTTCATGAGTCGA
TGAATGAGAAACAACAAAATGAGTTCCATAACAGCCGCGAGTGTAACTTTGCGATAGCCCGCGATGGGGTAGGGCGTTTC
CGTTGTTCAGCATTCTGGCAGCGCGACCAGGCAGGCATGGTTATTCGTCGGATCGTCACGCAGATTCCCCGCGTCGATGA
CCTCGGACTGCCGCCGGTACTGAAAGATATTATTATGTCCAAACGTGGTCTGGTGTTGTTTGTAGGGGGAACCGGCACCG
GTAAATCTACCTCTCTGGCGGCGCTTATCGGGCATCGAAACCAGAATTCCCACGGGCATATTCTGACCATTGAAGATCCG
ATTGAATTTGTTCACGAACACCGCAACTGTGTTATCACGCAGCGTGAAGTCGGGCTGGATACGCAAACCTTCGATGATGC
GCTGAAAAGCTCTTTGCGTCAGGCGCCGGATGTCATCCTGATAGGGGAAATCCGCTCGATGGAAACCATGGAGTATGCCA
TGTCGTTTGCGGATACGGGCCACCTTTGTGTGGCAACCCTGCACGCGAACAACGCCAACCAGGCAATTGAGCGTATTATG
CACCTGGCACCAAAAGATCAGCACGACAAATTGCGCTTCGATTTAAGCCTGAACATTCGTGCTATTGTGGCGCAGCAACT
GGTTCCTACCCGCGATGGCAAGGGGCGGGTTGCGGCTATTGAAATACTACTCAATTCACCGTTAGTGACAGATCTTATCC
AGCGCAATGAAATTGGCAGCCTCAAAGAGGCGATGAAAAAAGGGAAAGAGATGGGTATGCAGAGCTTTGATATGGCGCTG
TATGACCTTTACAAAGCCGGAAAAATCGAACTGGATCAGGCGTTGCATCATGCAGACTCGCCTAATGATCTGCGCCTGAT
GATTAAACTGGATACTAACGAAGGCTCAAGCCTGGGAACGTTATCTGACGTTTCTATCGACATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.945

98.103

0.588
  pilU Vibrio cholerae strain A1552

55.435

99.729

0.553
  pilU Acinetobacter baylyi ADP1

58

94.851

0.55
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.45

95.122

0.404
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.356

91.328

0.369
  pilT Vibrio cholerae strain A1552

40.356

91.328

0.369

Multiple sequence alignment