Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACMVO4_RS02010 Genome accession   NZ_CP180205
Coordinates   385953..386990 (+) Length   345 a.a.
NCBI ID   WP_004742512.1    Uniprot ID   F9SZF7
Organism   Vibrio europaeus strain EX1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 380953..391990
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMVO4_RS01970 (ACMVO4_01970) - 381433..381561 (+) 129 WP_414931847.1 hypothetical protein -
  ACMVO4_RS01975 (ACMVO4_01975) - 381575..381907 (-) 333 WP_414931848.1 hypothetical protein -
  ACMVO4_RS01980 (ACMVO4_01980) - 382100..382567 (+) 468 WP_414931849.1 hypothetical protein -
  ACMVO4_RS01985 (ACMVO4_01985) - 382900..383262 (-) 363 WP_414931850.1 hypothetical protein -
  ACMVO4_RS01990 (ACMVO4_01990) yggU 383480..383770 (-) 291 WP_069667663.1 DUF167 family protein YggU -
  ACMVO4_RS01995 (ACMVO4_01995) - 383770..384327 (-) 558 WP_171801040.1 YggT family protein -
  ACMVO4_RS02000 (ACMVO4_02000) proC 384367..385185 (-) 819 WP_171801041.1 pyrroline-5-carboxylate reductase -
  ACMVO4_RS02005 (ACMVO4_02005) - 385211..385927 (-) 717 WP_069667660.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACMVO4_RS02010 (ACMVO4_02010) pilT 385953..386990 (+) 1038 WP_004742512.1 type IV pilus twitching motility protein PilT Machinery gene
  ACMVO4_RS02015 (ACMVO4_02015) pilU 387008..388114 (+) 1107 WP_171801043.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACMVO4_RS02020 (ACMVO4_02020) - 388197..388352 (-) 156 WP_171382716.1 FeoB-associated Cys-rich membrane protein -
  ACMVO4_RS02025 (ACMVO4_02025) feoB 388355..390856 (-) 2502 WP_414931851.1 ferrous iron transport protein B -
  ACMVO4_RS02030 (ACMVO4_02030) - 390880..391110 (-) 231 WP_004742516.1 FeoA family protein -
  ACMVO4_RS02035 (ACMVO4_02035) - 391330..391641 (+) 312 WP_069667657.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38184.60 Da        Isoelectric Point: 6.1455

>NTDB_id=1094495 ACMVO4_RS02010 WP_004742512.1 385953..386990(+) (pilT) [Vibrio europaeus strain EX1]
MDIAELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLGVPAFSHADVHRLVFEIMNDGQRSEFEERLEVDFSFELPNVGR
FRVNAFNQARGCAAVFRTIPTEIPTLEQLSAPQIFESISNMEKGLVLVTGPTGSGKSTTLAAMVDHINRNHNKHILTIED
PIEFVHSNNKCLINQREVHRDTHSFNNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRAVIAQKLLKRIGGGRTACHEIMMATPAIRNLIREDKVAQMFSVIQTGAAHGMQTMEQNA
KQLIAQGLVDQDEVAKKIELETSMF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1094495 ACMVO4_RS02010 WP_004742512.1 385953..386990(+) (pilT) [Vibrio europaeus strain EX1]
ATGGATATCGCCGAATTACTGGATTTTAGTGTAAAGCATAATGCTTCAGATCTACATCTTTCTGCCGGAGTATCTCCAAT
GGTACGGATAGATGGTGAAGTAAGAAAGCTTGGCGTACCTGCTTTTAGCCATGCAGACGTGCATCGTTTAGTATTCGAAA
TTATGAATGATGGTCAACGCAGTGAATTTGAAGAGCGCCTTGAAGTCGACTTTTCTTTCGAGCTGCCCAATGTTGGTCGC
TTCCGTGTTAATGCATTTAACCAAGCGCGTGGTTGCGCGGCGGTATTTCGTACCATCCCGACCGAGATCCCGACCTTAGA
GCAACTGTCAGCCCCGCAAATCTTCGAAAGTATCTCCAATATGGAAAAGGGTTTGGTATTGGTGACTGGCCCTACAGGCT
CTGGTAAATCAACCACTTTGGCGGCAATGGTTGACCATATCAACCGTAATCACAATAAGCATATTCTAACGATTGAAGAC
CCAATTGAATTTGTTCATAGCAATAATAAGTGCTTAATCAACCAACGCGAAGTTCATCGAGATACGCATAGTTTTAATAA
TGCGCTGCGCAGTGCACTGCGTGAAGACCCAGACGTGATCTTAGTGGGTGAGTTACGTGACCAAGAGACAATCAGCCTAG
CACTGACAGCGGCAGAAACCGGGCACTTGGTGTTTGGTACTTTGCATACTAGCTCAGCGGCAAAAACCATCGACCGTATT
ATTGATGTTTTCCCGGGTAGTGATAAGGATATGGTTCGCTCTATGCTCTCTGAGTCATTACGCGCAGTCATTGCACAAAA
GCTGCTGAAGCGTATTGGCGGTGGCCGGACCGCGTGTCATGAGATCATGATGGCGACACCGGCGATCCGTAACTTGATCC
GTGAAGACAAGGTTGCTCAGATGTTTTCTGTGATTCAAACCGGTGCCGCACATGGTATGCAAACCATGGAGCAAAATGCC
AAGCAGTTGATCGCACAAGGTCTCGTTGATCAAGATGAGGTGGCGAAGAAGATCGAACTTGAAACTTCAATGTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F9SZF7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

88.986

100

0.89
  pilT Vibrio cholerae strain A1552

88.986

100

0.89
  pilT Acinetobacter baumannii D1279779

72.34

95.362

0.69
  pilT Acinetobacter baumannii strain A118

72.34

95.362

0.69
  pilT Acinetobacter nosocomialis M2

72.036

95.362

0.687
  pilT Acinetobacter baylyi ADP1

70.606

95.652

0.675
  pilT Pseudomonas stutzeri DSM 10701

67.953

97.681

0.664
  pilT Pseudomonas aeruginosa PAK

66.765

98.551

0.658
  pilT Legionella pneumophila strain Lp02

65.507

100

0.655
  pilT Legionella pneumophila strain ERS1305867

65.507

100

0.655
  pilT Neisseria meningitidis 8013

64.793

97.971

0.635
  pilT Neisseria gonorrhoeae MS11

62.899

100

0.629
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.497

96.812

0.499
  pilU Vibrio cholerae strain A1552

42.154

94.203

0.397
  pilU Pseudomonas stutzeri DSM 10701

41.159

95.072

0.391
  pilU Acinetobacter baylyi ADP1

40.312

92.754

0.374

Multiple sequence alignment