Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACMVO4_RS02015 Genome accession   NZ_CP180205
Coordinates   387008..388114 (+) Length   368 a.a.
NCBI ID   WP_171801043.1    Uniprot ID   A0AAE7AT79
Organism   Vibrio europaeus strain EX1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 382008..393114
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMVO4_RS01980 (ACMVO4_01980) - 382100..382567 (+) 468 WP_414931849.1 hypothetical protein -
  ACMVO4_RS01985 (ACMVO4_01985) - 382900..383262 (-) 363 WP_414931850.1 hypothetical protein -
  ACMVO4_RS01990 (ACMVO4_01990) yggU 383480..383770 (-) 291 WP_069667663.1 DUF167 family protein YggU -
  ACMVO4_RS01995 (ACMVO4_01995) - 383770..384327 (-) 558 WP_171801040.1 YggT family protein -
  ACMVO4_RS02000 (ACMVO4_02000) proC 384367..385185 (-) 819 WP_171801041.1 pyrroline-5-carboxylate reductase -
  ACMVO4_RS02005 (ACMVO4_02005) - 385211..385927 (-) 717 WP_069667660.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACMVO4_RS02010 (ACMVO4_02010) pilT 385953..386990 (+) 1038 WP_004742512.1 type IV pilus twitching motility protein PilT Machinery gene
  ACMVO4_RS02015 (ACMVO4_02015) pilU 387008..388114 (+) 1107 WP_171801043.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACMVO4_RS02020 (ACMVO4_02020) - 388197..388352 (-) 156 WP_171382716.1 FeoB-associated Cys-rich membrane protein -
  ACMVO4_RS02025 (ACMVO4_02025) feoB 388355..390856 (-) 2502 WP_414931851.1 ferrous iron transport protein B -
  ACMVO4_RS02030 (ACMVO4_02030) - 390880..391110 (-) 231 WP_004742516.1 FeoA family protein -
  ACMVO4_RS02035 (ACMVO4_02035) - 391330..391641 (+) 312 WP_069667657.1 hypothetical protein -
  ACMVO4_RS02040 (ACMVO4_02040) - 391667..392452 (+) 786 WP_171382718.1 DUF4198 domain-containing protein -
  ACMVO4_RS02045 (ACMVO4_02045) - 392549..392857 (+) 309 WP_171801045.1 hypothetical protein -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41331.08 Da        Isoelectric Point: 6.0185

>NTDB_id=1094496 ACMVO4_RS02015 WP_171801043.1 387008..388114(+) (pilU) [Vibrio europaeus strain EX1]
MDIDVFLQQMIDQNASDLYITVDAPILYRVDGELRAQGDKLSHAVVSSLLDGIMDHDRQHEFHSSKEANFAIVRHFGRFR
VSAFYQRELPGAVIRRIETKIPTFDELKLPDVLQDLSIAKRGLVLVVGATGSGKSTTMAAMTGFRNSQRTGHILTVEDPI
EFVHEHQRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRTRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLRGVIGQQLIRDKNGQGRHGVFEVLLNSPRVSDLIRRGDLHELKATMAKSKEVGMQTFDQALY
QLVVEDKISEEDAMHSADSANDLRLMLKTQRGDVSSSGSLSGVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=1094496 ACMVO4_RS02015 WP_171801043.1 387008..388114(+) (pilU) [Vibrio europaeus strain EX1]
ATGGATATTGATGTTTTTTTGCAACAGATGATCGACCAAAACGCGTCTGATCTTTACATCACAGTTGATGCGCCAATTCT
TTACCGTGTCGATGGAGAGCTGCGTGCACAAGGAGACAAACTATCACACGCTGTTGTGAGCTCTTTGTTGGATGGGATTA
TGGATCATGATCGCCAACACGAGTTTCATTCGAGCAAAGAAGCGAACTTTGCCATTGTGCGTCACTTTGGTCGATTCCGT
GTCTCTGCATTCTATCAGCGCGAATTGCCCGGTGCGGTGATTCGCCGAATTGAAACCAAGATTCCGACCTTTGATGAGCT
TAAATTACCGGATGTTTTGCAAGACTTATCGATTGCGAAACGTGGTTTGGTGCTGGTGGTTGGTGCTACGGGCTCGGGTA
AGTCAACAACCATGGCCGCAATGACTGGGTTCCGTAATAGTCAGCGGACAGGGCATATTTTGACGGTGGAAGACCCGATA
GAGTTTGTTCATGAGCATCAGCGTTGTATTGTGACACAACGTGAGGTGGGGTTAGATACCGAAAGCTATGAAGTGGCGTT
GAAGAACTCACTGCGTCAAGCTCCAGATATGATCTTAATAGGGGAGATTCGTACACGAGAGACGATGGAATATGCAATGA
CCTTTGCTGAAACTGGGCACCTGTGTATGGCAACCCTTCATGCAAACAATGCTAATCAAGCGTTAGAGCGGATCCTTCAT
TTGGTGCCTAAGGAGCAAAAAGAACAGTTCCTATTTGATTTATCGATGAACCTACGTGGTGTGATAGGTCAGCAACTTAT
TCGAGATAAAAATGGTCAAGGTCGCCACGGCGTGTTTGAAGTTTTACTCAACAGCCCACGCGTGTCGGATCTGATTCGCC
GCGGCGATCTGCATGAGCTTAAAGCGACCATGGCTAAGTCGAAAGAGGTTGGCATGCAAACTTTTGATCAGGCACTTTAT
CAGCTGGTTGTGGAAGACAAGATCAGTGAAGAAGATGCCATGCATAGTGCGGATTCAGCCAATGATTTACGTCTAATGCT
TAAAACACAACGTGGTGATGTGTCTAGCTCCGGATCACTCAGTGGCGTGAAGATTGATATGGACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

82.609

100

0.826
  pilU Pseudomonas stutzeri DSM 10701

57.143

95.109

0.543
  pilU Acinetobacter baylyi ADP1

53.276

95.38

0.508
  pilT Pseudomonas aeruginosa PAK

41.888

92.12

0.386
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.888

92.12

0.386
  pilT Legionella pneumophila strain Lp02

41.003

92.12

0.378
  pilT Legionella pneumophila strain ERS1305867

41.003

92.12

0.378
  pilT Acinetobacter baylyi ADP1

40.762

92.663

0.378
  pilT Acinetobacter baumannii D1279779

40.708

92.12

0.375
  pilT Acinetobacter baumannii strain A118

40.708

92.12

0.375
  pilT Acinetobacter nosocomialis M2

40.708

92.12

0.375
  pilT Pseudomonas stutzeri DSM 10701

40.118

92.12

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.923

88.315

0.361
  pilT Vibrio cholerae strain A1552

40.923

88.315

0.361

Multiple sequence alignment