Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACMDW7_RS06095 Genome accession   NZ_CP180191
Coordinates   1358543..1359583 (+) Length   346 a.a.
NCBI ID   WP_377302359.1    Uniprot ID   -
Organism   Piscinibacterium candidicorallinum strain LMG 29480     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1353543..1364583
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMDW7_RS06080 (ACMDW7_06080) - 1354810..1356336 (+) 1527 WP_377302365.1 DEAD/DEAH box helicase -
  ACMDW7_RS06085 (ACMDW7_06085) gluQRS 1356717..1357574 (-) 858 WP_377302363.1 tRNA glutamyl-Q(34) synthetase GluQRS -
  ACMDW7_RS06090 (ACMDW7_06090) - 1357587..1358315 (-) 729 WP_377302361.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACMDW7_RS06095 (ACMDW7_06095) pilT 1358543..1359583 (+) 1041 WP_377302359.1 type IV pilus twitching motility protein PilT Machinery gene
  ACMDW7_RS06100 (ACMDW7_06100) pilU 1359667..1360800 (+) 1134 WP_054126788.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACMDW7_RS06105 (ACMDW7_06105) - 1360818..1361297 (+) 480 WP_377302357.1 glutathione peroxidase -
  ACMDW7_RS06115 (ACMDW7_06115) scpB 1361719..1362561 (+) 843 WP_377302355.1 SMC-Scp complex subunit ScpB -
  ACMDW7_RS06120 (ACMDW7_06120) - 1362558..1364024 (+) 1467 WP_377302353.1 pseudouridine synthase -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38415.22 Da        Isoelectric Point: 7.0667

>NTDB_id=1094369 ACMDW7_RS06095 WP_377302359.1 1358543..1359583(+) (pilT) [Piscinibacterium candidicorallinum strain LMG 29480]
MDITELLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINLPALTHEDVHRMIYDIMNDAQRKQYEEIFECDFSFSIPGLAR
FRVNAFVQNRGAGAVFRTIPSKVLTLQDLNAPRIFQDMSMQPRGIVLVTGPTGSGKSTTLAAMVNHINDNIFGHILTVED
PIEFVHESKKCLINQREVGPHTLSFNNSLRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAAEKDMVRAMLSESLRAVISQSLLKTKDGSGRVAAHEIMIGTPAIRNLIREAKVAQMYSMIQAGQQFGMQTLDQC
LLDLVRRNVVSAEEARVYAQNKENFG

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=1094369 ACMDW7_RS06095 WP_377302359.1 1358543..1359583(+) (pilT) [Piscinibacterium candidicorallinum strain LMG 29480]
ATGGATATCACCGAACTGCTGGCCTTCTCGGTCAAGAACAAGGCCTCCGACCTGCACCTGTCCGCCGGCCTGCCGCCCAT
GATCCGCGTGCACGGCGACGTGCGGCGCATCAACCTGCCGGCACTCACGCACGAGGACGTGCATCGCATGATCTATGACA
TCATGAACGATGCCCAGCGCAAGCAGTACGAGGAGATCTTCGAGTGCGACTTCTCGTTCTCGATCCCCGGCCTCGCGCGT
TTCCGCGTCAATGCCTTCGTGCAGAACCGCGGTGCGGGCGCGGTGTTCCGGACGATTCCGTCCAAGGTACTCACGCTGCA
GGATCTGAATGCACCACGCATCTTCCAGGACATGTCGATGCAGCCGCGCGGCATCGTGCTGGTGACTGGCCCCACGGGTT
CGGGCAAGTCCACCACCCTGGCCGCGATGGTCAACCACATCAACGACAACATCTTCGGCCACATCCTCACCGTCGAAGAC
CCGATCGAATTCGTGCACGAGAGCAAGAAGTGCCTCATCAACCAGCGTGAGGTCGGCCCTCACACCCTGTCGTTCAACAA
CTCTCTGCGCTCCGCGCTGCGCGAAGACCCTGACGTGATTCTGGTGGGTGAGTTGCGCGACCTCGAGACCATCCGCCTGG
CACTCACCGCCGCGGAAACCGGCCACCTGGTGTTTGGCACCCTGCACACGTCCTCGGCCGCCAAGACCATCGACCGCATC
ATTGACGTGTTCCCCGCCGCGGAAAAAGACATGGTCCGCGCCATGCTCTCCGAATCCCTGCGCGCGGTGATCTCGCAGAG
CCTGCTCAAGACCAAGGACGGCTCGGGTCGCGTGGCCGCGCACGAGATCATGATCGGCACGCCCGCCATCCGCAACCTCA
TCCGCGAGGCCAAGGTCGCGCAGATGTACTCCATGATCCAGGCCGGCCAGCAGTTCGGCATGCAGACCCTGGACCAGTGC
CTGCTCGACCTCGTGCGCCGTAACGTGGTCAGCGCCGAGGAAGCACGCGTCTACGCCCAGAACAAGGAAAACTTCGGCTG
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.391

99.711

0.772
  pilT Acinetobacter baumannii D1279779

77.391

99.711

0.772
  pilT Acinetobacter baumannii strain A118

77.391

99.711

0.772
  pilT Acinetobacter baylyi ADP1

74.783

99.711

0.746
  pilT Pseudomonas aeruginosa PAK

74.493

99.711

0.743
  pilT Pseudomonas stutzeri DSM 10701

73.913

99.711

0.737
  pilT Legionella pneumophila strain Lp02

72.464

99.711

0.723
  pilT Legionella pneumophila strain ERS1305867

72.464

99.711

0.723
  pilT Neisseria meningitidis 8013

68.986

99.711

0.688
  pilT Neisseria gonorrhoeae MS11

68.696

99.711

0.685
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.238

97.11

0.682
  pilT Vibrio cholerae strain A1552

70.238

97.11

0.682
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.145

99.711

0.5
  pilU Acinetobacter baylyi ADP1

41.329

100

0.413
  pilU Pseudomonas stutzeri DSM 10701

40.58

99.711

0.405
  pilU Vibrio cholerae strain A1552

40.896

96.821

0.396

Multiple sequence alignment