Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACMDW7_RS06100 Genome accession   NZ_CP180191
Coordinates   1359667..1360800 (+) Length   377 a.a.
NCBI ID   WP_054126788.1    Uniprot ID   -
Organism   Piscinibacterium candidicorallinum strain LMG 29480     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1354667..1365800
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMDW7_RS06080 (ACMDW7_06080) - 1354810..1356336 (+) 1527 WP_377302365.1 DEAD/DEAH box helicase -
  ACMDW7_RS06085 (ACMDW7_06085) gluQRS 1356717..1357574 (-) 858 WP_377302363.1 tRNA glutamyl-Q(34) synthetase GluQRS -
  ACMDW7_RS06090 (ACMDW7_06090) - 1357587..1358315 (-) 729 WP_377302361.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACMDW7_RS06095 (ACMDW7_06095) pilT 1358543..1359583 (+) 1041 WP_377302359.1 type IV pilus twitching motility protein PilT Machinery gene
  ACMDW7_RS06100 (ACMDW7_06100) pilU 1359667..1360800 (+) 1134 WP_054126788.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACMDW7_RS06105 (ACMDW7_06105) - 1360818..1361297 (+) 480 WP_377302357.1 glutathione peroxidase -
  ACMDW7_RS06115 (ACMDW7_06115) scpB 1361719..1362561 (+) 843 WP_377302355.1 SMC-Scp complex subunit ScpB -
  ACMDW7_RS06120 (ACMDW7_06120) - 1362558..1364024 (+) 1467 WP_377302353.1 pseudouridine synthase -
  ACMDW7_RS06125 (ACMDW7_06125) rimP 1364254..1364742 (+) 489 WP_377302351.1 ribosome maturation factor RimP -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41956.54 Da        Isoelectric Point: 6.9239

>NTDB_id=1094370 ACMDW7_RS06100 WP_054126788.1 1359667..1360800(+) (pilU) [Piscinibacterium candidicorallinum strain LMG 29480]
MERDQATKFMHDLLKLMLQKRGSDLFITAAFPPAFKIDGRVVPVSNQALTHVHTAELARAIMNDKQAAEFENKMECNFAI
NPSGIGRFRCNAFVQQGMVGMVLRTIPANIPTVEGMGLPPQLKDIVMAKRGIVLLVGATGSGKSTSMAAMLRHRNETTHG
HIITIEDPIEFVHPHANCIISQREVGVDTESWEAALKNALRQAPDVVQIGEIRDKDTMENAIVFAETGHLCMATLHANNS
NQAIDRIINFFPEEKRQQLLMDLSLNLRAMISQRLIPLKDSKGRAAACEILINSPLISDMIFKGEVGEIKDVMKRSRELG
MQTFDQHLFDLFEDGKISYEDALRNADSVNDLRLRIKLESKLAQRDPSLGVGHLSIV

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=1094370 ACMDW7_RS06100 WP_054126788.1 1359667..1360800(+) (pilU) [Piscinibacterium candidicorallinum strain LMG 29480]
ATGGAACGCGATCAGGCCACCAAGTTCATGCACGACCTGCTGAAGCTCATGCTTCAGAAGCGTGGCTCGGACCTCTTCAT
CACCGCTGCCTTTCCGCCGGCCTTCAAGATCGACGGCCGCGTGGTGCCCGTTTCGAATCAGGCGCTGACGCATGTGCACA
CCGCCGAACTGGCGCGCGCCATCATGAACGACAAGCAGGCCGCCGAGTTCGAGAACAAGATGGAGTGCAACTTCGCCATC
AACCCCTCGGGTATCGGCCGCTTCCGCTGCAATGCATTTGTGCAGCAGGGCATGGTGGGCATGGTGCTGCGGACGATTCC
GGCCAATATTCCCACCGTGGAAGGCATGGGTCTGCCGCCGCAACTCAAGGACATCGTGATGGCCAAGCGCGGCATCGTGC
TGCTTGTGGGCGCGACAGGTTCGGGCAAGTCCACCTCGATGGCGGCCATGCTGCGGCATCGCAACGAAACCACCCACGGC
CACATCATCACGATCGAAGACCCCATCGAGTTCGTGCACCCGCATGCCAACTGCATCATTTCGCAGCGCGAAGTCGGCGT
GGACACGGAGTCCTGGGAGGCCGCACTGAAGAACGCCCTGCGTCAGGCGCCCGATGTGGTTCAGATCGGCGAAATCCGCG
ACAAGGACACGATGGAGAACGCCATCGTCTTTGCCGAAACCGGCCACCTCTGCATGGCCACGCTGCACGCCAACAACTCC
AACCAGGCCATCGACCGGATCATCAACTTCTTCCCGGAAGAAAAGCGCCAGCAGCTGCTGATGGACCTGTCACTGAACCT
GCGCGCCATGATCTCCCAGCGCCTGATCCCGCTGAAGGACTCCAAGGGCCGCGCCGCCGCCTGCGAGATCCTGATCAACT
CGCCGCTCATCTCAGACATGATCTTCAAGGGCGAGGTCGGCGAGATCAAGGACGTGATGAAGCGTTCGCGCGAACTGGGC
ATGCAGACTTTCGACCAGCACCTTTTCGATCTCTTCGAAGACGGCAAGATCAGCTACGAAGATGCGCTGCGCAACGCCGA
TAGCGTCAACGACCTGCGTCTGCGCATCAAGCTCGAATCCAAACTCGCGCAGCGCGACCCCTCGCTCGGCGTGGGCCACC
TCTCGATTGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(16-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.885

92.573

0.554
  pilU Acinetobacter baylyi ADP1

58

92.838

0.538
  pilU Vibrio cholerae strain A1552

52.446

97.613

0.512
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.075

88.859

0.401
  pilT Acinetobacter baumannii D1279779

43.202

87.798

0.379
  pilT Acinetobacter nosocomialis M2

43.202

87.798

0.379
  pilT Acinetobacter baumannii strain A118

43.202

87.798

0.379
  pilT Pseudomonas aeruginosa PAK

42.09

88.859

0.374
  pilT Acinetobacter baylyi ADP1

42.598

87.798

0.374
  pilT Pseudomonas stutzeri DSM 10701

41.493

88.859

0.369

Multiple sequence alignment