Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACMHYC_RS05135 Genome accession   NZ_CP180027
Coordinates   1081655..1082692 (-) Length   345 a.a.
NCBI ID   WP_004770978.1    Uniprot ID   N9LTM1
Organism   Acinetobacter courvalinii isolate 2024CK-01798     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1076655..1087692
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMHYC_RS05105 (ACMHYC_05105) - 1077304..1077759 (+) 456 WP_004770972.1 bacteriohemerythrin -
  ACMHYC_RS05110 (ACMHYC_05110) - 1078007..1079089 (+) 1083 WP_199949217.1 hypothetical protein -
  ACMHYC_RS05115 (ACMHYC_05115) - 1079090..1079413 (+) 324 WP_199949218.1 RnfH family protein -
  ACMHYC_RS05120 (ACMHYC_05120) - 1079467..1079865 (-) 399 WP_004655148.1 outer membrane protein assembly factor BamE -
  ACMHYC_RS05125 (ACMHYC_05125) fur 1079977..1080414 (+) 438 WP_004770976.1 ferric iron uptake transcriptional regulator -
  ACMHYC_RS05130 (ACMHYC_05130) pilU 1080512..1081630 (-) 1119 WP_004770977.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACMHYC_RS05135 (ACMHYC_05135) pilT 1081655..1082692 (-) 1038 WP_004770978.1 type IV pilus twitching motility protein PilT Machinery gene
  ACMHYC_RS05140 (ACMHYC_05140) - 1082820..1083512 (+) 693 WP_199949220.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACMHYC_RS05145 (ACMHYC_05145) - 1083548..1084213 (-) 666 WP_199949221.1 HAD family hydrolase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38312.95 Da        Isoelectric Point: 6.5054

>NTDB_id=1094089 ACMHYC_RS05135 WP_004770978.1 1081655..1082692(-) (pilT) [Acinetobacter courvalinii isolate 2024CK-01798]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKDVHRLVYDIMNDKQRRDFEEDLETDFSFEVPNIAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGTIFKEICDYPRGIVLVTGPTGSGKSTTLAAMIDYINENRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNAALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLVRENKVAQMYSAIQTGANYGMTTLDQSL
KTLVSKGLISPQTARTVAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1094089 ACMHYC_RS05135 WP_004770978.1 1081655..1082692(-) (pilT) [Acinetobacter courvalinii isolate 2024CK-01798]
ATGGATATTACAGAATTACTCGCCTTTTCGGTTAAAAACGGTGCGTCAGATTTACACCTGTCAGCGGGTATGCCTCCGAT
GATTCGCGTAGATGGTGAAGTCCGTCGTATCAACTTGCCAGCATTGGAACATAAAGATGTACACCGTCTAGTGTACGACA
TTATGAATGATAAACAACGTCGTGATTTTGAAGAAGACCTAGAAACTGACTTTTCATTTGAAGTACCGAATATTGCCCGT
TTCCGTGTGAATGCTTTCAACCAGAACCGTGGCGCTGGTGCGGTGTTCCGTACCATTCCTTCTAAAGTCCTGACGATGGA
AGATTTAGGCCTCGGTACAATTTTCAAGGAAATCTGTGATTATCCACGTGGAATTGTGTTGGTGACAGGCCCGACAGGTT
CGGGTAAGTCGACCACCCTTGCAGCAATGATCGACTATATTAATGAAAACCGTTATGACCATATCCTCACGGTAGAAGAT
CCAATCGAATTTGTACACCAGTCGAAGAAATGCCTGATCAACCAGCGTGAAGTGCACCGTGATACGCATGGCTTTAATGC
CGCACTGCGCTCTGCCCTGCGTGAAGACCCGGATATTATTCTGGTCGGTGAGATGCGTGACTTGGAAACCATTCGTCTGG
CATTGACCGCTGCGGAAACTGGTCACCTAGTATTTGGTACCCTGCATACCACCTCTGCCGCAAAAACCATTGACCGTGTC
ATCGACGTATTCCCTGCTGAAGAAAAAGATATGGTGCGTGCCATGTTGTCTGAATCTCTACAAGCGGTGATTTCACAAAC
CTTATTGAAAAAGAACGGCGGTGGCCGTGTGGCTGCACATGAAATCATGATCGGGATTCCGGCAATCCGTAACCTTGTGC
GTGAAAATAAAGTCGCGCAAATGTACTCAGCGATCCAGACAGGTGCCAACTACGGTATGACCACCTTGGATCAAAGTTTG
AAAACATTGGTGTCCAAAGGTTTAATCAGCCCGCAAACGGCACGTACCGTAGCGAAACAACCCGAGTCATTCCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9LTM1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

96.232

100

0.962
  pilT Acinetobacter baumannii strain A118

95.942

100

0.959
  pilT Acinetobacter baumannii D1279779

95.942

100

0.959
  pilT Acinetobacter baylyi ADP1

87.209

99.71

0.87
  pilT Pseudomonas aeruginosa PAK

83.43

99.71

0.832
  pilT Pseudomonas stutzeri DSM 10701

83.14

99.71

0.829
  pilT Legionella pneumophila strain Lp02

75.872

99.71

0.757
  pilT Legionella pneumophila strain ERS1305867

75.872

99.71

0.757
  pilT Neisseria meningitidis 8013

67.826

100

0.678
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.345

97.391

0.675
  pilT Vibrio cholerae strain A1552

69.345

97.391

0.675
  pilT Neisseria gonorrhoeae MS11

67.536

100

0.675
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

100

0.513
  pilU Vibrio cholerae strain A1552

41.791

97.101

0.406
  pilU Pseudomonas stutzeri DSM 10701

41.194

97.101

0.4
  pilU Acinetobacter baylyi ADP1

39.306

100

0.394

Multiple sequence alignment