Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACMHYC_RS05130 Genome accession   NZ_CP180027
Coordinates   1080512..1081630 (-) Length   372 a.a.
NCBI ID   WP_004770977.1    Uniprot ID   N8WCM5
Organism   Acinetobacter courvalinii isolate 2024CK-01798     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1075512..1086630
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMHYC_RS05100 (ACMHYC_05100) - 1076170..1077018 (+) 849 WP_199949216.1 class II glutamine amidotransferase -
  ACMHYC_RS05105 (ACMHYC_05105) - 1077304..1077759 (+) 456 WP_004770972.1 bacteriohemerythrin -
  ACMHYC_RS05110 (ACMHYC_05110) - 1078007..1079089 (+) 1083 WP_199949217.1 hypothetical protein -
  ACMHYC_RS05115 (ACMHYC_05115) - 1079090..1079413 (+) 324 WP_199949218.1 RnfH family protein -
  ACMHYC_RS05120 (ACMHYC_05120) - 1079467..1079865 (-) 399 WP_004655148.1 outer membrane protein assembly factor BamE -
  ACMHYC_RS05125 (ACMHYC_05125) fur 1079977..1080414 (+) 438 WP_004770976.1 ferric iron uptake transcriptional regulator -
  ACMHYC_RS05130 (ACMHYC_05130) pilU 1080512..1081630 (-) 1119 WP_004770977.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACMHYC_RS05135 (ACMHYC_05135) pilT 1081655..1082692 (-) 1038 WP_004770978.1 type IV pilus twitching motility protein PilT Machinery gene
  ACMHYC_RS05140 (ACMHYC_05140) - 1082820..1083512 (+) 693 WP_199949220.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACMHYC_RS05145 (ACMHYC_05145) - 1083548..1084213 (-) 666 WP_199949221.1 HAD family hydrolase -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41722.91 Da        Isoelectric Point: 6.3489

>NTDB_id=1094088 ACMHYC_RS05130 WP_004770977.1 1080512..1081630(-) (pilU) [Acinetobacter courvalinii isolate 2024CK-01798]
MDFNDLLNLMIQQNASDLFVTADVEPSMKINGQIVPVSKTKLSGEIVGQLVQSIMTDKQRKEFADTRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRKIETHIPTMDDLKLPEVLKELAMTKRGIIIFVGATGTGKSTSLASMIGFRNQNSKGHIITIE
DPIEFVHQHAGCIVTQREVGIDTDSFEVALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVLATLHANNANQALDR
IIHFFEADRHSQLYMDLSLNLKALVAQQLIPTPDGNSRRAAIEILINTPLLADYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLSEEGADQLLSASRNITFDGQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=1094088 ACMHYC_RS05130 WP_004770977.1 1080512..1081630(-) (pilU) [Acinetobacter courvalinii isolate 2024CK-01798]
ATGGATTTTAATGACTTACTCAATTTAATGATTCAGCAGAATGCCTCAGACTTATTTGTCACGGCGGATGTTGAACCTTC
AATGAAAATTAACGGGCAGATTGTGCCTGTTTCAAAAACCAAGCTGTCTGGGGAAATCGTCGGTCAACTGGTACAGTCCA
TCATGACGGACAAACAACGTAAAGAATTTGCCGATACCCGTGAATGTAACTTCGCGATCATGAATCGTGAAAAAACGGCC
CGTTTCCGTGTCAGTGCCTTTCAGCAACGTGATATGCCGGGCATGGTTCTGCGTAAGATCGAGACCCACATCCCCACCAT
GGATGACCTGAAACTGCCAGAAGTACTGAAAGAACTGGCAATGACCAAACGCGGTATCATCATTTTTGTTGGTGCAACGG
GTACGGGTAAATCAACCTCTCTCGCTTCGATGATTGGTTTCCGTAACCAGAATTCCAAAGGCCATATTATTACCATTGAA
GACCCGATCGAATTCGTACACCAACATGCAGGTTGCATTGTGACGCAACGCGAAGTCGGCATTGATACCGATTCATTTGA
AGTGGCATTGAAAAATACGCTGCGTCAGGCACCGGATGTGATCCTGATTGGTGAGATCCGTTCGCGTGAAACCATGGACT
ACGCGATTGCCTTCGCAGAAACAGGACACTTGGTACTCGCGACCCTGCACGCCAACAATGCCAACCAGGCATTGGATCGT
ATTATTCACTTCTTTGAAGCTGATCGTCACAGCCAACTGTATATGGACTTGTCGCTGAACTTAAAAGCGTTAGTGGCGCA
GCAGCTAATTCCAACACCTGATGGTAATTCACGTCGTGCTGCGATTGAGATTCTGATTAACACCCCGCTTCTGGCCGACT
ATATCCGTAAGGGAGAAATTCACGAGATTAAAGACCTGATGAAGCGTTCACGTGAGTTGGGTATGCAAACCTTTGACCAA
GCCCTATTCGACCTTTATAAGGCAGGACAGATCACTTACAAAGATGCCTTAAAGCATGCCGATTCGCCAAACGACTTGCG
TCTCACCATCAAACTTTCCGAAGAAGGCGCAGACCAGTTACTGAGCGCCAGCCGTAACATTACCTTCGATGGGCAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-250)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N8WCM5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

76.19

100

0.774
  pilU Pseudomonas stutzeri DSM 10701

70.62

99.731

0.704
  pilU Vibrio cholerae strain A1552

54.324

99.462

0.54
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.618

93.011

0.387
  pilT Legionella pneumophila strain ERS1305867

40.476

90.323

0.366
  pilT Legionella pneumophila strain Lp02

40.476

90.323

0.366
  pilT Pseudomonas aeruginosa PAK

39.306

93.011

0.366
  pilT Neisseria gonorrhoeae MS11

39.706

91.398

0.363
  pilT Neisseria meningitidis 8013

39.706

91.398

0.363
  pilT Pseudomonas stutzeri DSM 10701

39.017

93.011

0.363
  pilT Acinetobacter baumannii D1279779

39.067

92.204

0.36
  pilT Acinetobacter baumannii strain A118

39.067

92.204

0.36
  pilT Acinetobacter nosocomialis M2

39.067

92.204

0.36

Multiple sequence alignment