Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACMG4L_RS12625 Genome accession   NZ_CP179933
Coordinates   2710344..2711378 (-) Length   344 a.a.
NCBI ID   WP_272962781.1    Uniprot ID   A0A2D5DPV3
Organism   Alcanivorax sp. IL1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2705344..2716378
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMG4L_RS12595 (ACMG4L_12595) - 2705758..2706678 (-) 921 WP_414430505.1 glutathione S-transferase family protein -
  ACMG4L_RS12600 (ACMG4L_12600) - 2706748..2707302 (-) 555 WP_414430504.1 YaeQ family protein -
  ACMG4L_RS12605 (ACMG4L_12605) - 2707397..2707948 (-) 552 WP_035249732.1 TMEM165/GDT1 family protein -
  ACMG4L_RS12610 (ACMG4L_12610) - 2708296..2708505 (+) 210 WP_035249734.1 SlyX family protein -
  ACMG4L_RS12615 (ACMG4L_12615) - 2708661..2709086 (-) 426 WP_035249736.1 TM2 domain-containing protein -
  ACMG4L_RS12620 (ACMG4L_12620) pilU 2709207..2710328 (-) 1122 WP_035249738.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACMG4L_RS12625 (ACMG4L_12625) pilT 2710344..2711378 (-) 1035 WP_272962781.1 type IV pilus twitching motility protein PilT Machinery gene
  ACMG4L_RS12630 (ACMG4L_12630) - 2711515..2712198 (+) 684 WP_414430503.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACMG4L_RS12635 (ACMG4L_12635) proC 2712272..2713081 (+) 810 WP_414430502.1 pyrroline-5-carboxylate reductase -
  ACMG4L_RS12640 (ACMG4L_12640) - 2713378..2713926 (+) 549 WP_272962778.1 YggT family protein -
  ACMG4L_RS12645 (ACMG4L_12645) - 2714348..2715814 (+) 1467 WP_414430501.1 diguanylate cyclase domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37903.54 Da        Isoelectric Point: 6.3485

>NTDB_id=1093988 ACMG4L_RS12625 WP_272962781.1 2710344..2711378(-) (pilT) [Alcanivorax sp. IL1]
MDITELLAFSAKNGASDLHLSAGLPPMIRVDGDVRRINLPAMEHKEVHALIYDIMNDKQRKDFEEFFETDFSFEVPGVAR
FRVNAFNHNRGAGAVFRTIPSKVLTMEDLGMGKVFQKISDMPRGIVLVTGPTGSGKSTTLAAMVDYINDSRYEHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMIRSMLSESLQAVVSQTLLKKNGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGGALGMQTLDQCL
QGLVNKGLVARDVAREKAKSPDSI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1093988 ACMG4L_RS12625 WP_272962781.1 2710344..2711378(-) (pilT) [Alcanivorax sp. IL1]
ATGGATATTACCGAGCTGCTCGCTTTCAGCGCCAAAAACGGCGCTTCCGACCTTCACCTGTCCGCCGGTCTGCCACCGAT
GATTCGTGTGGACGGCGACGTGCGCCGCATCAATCTGCCGGCCATGGAGCACAAGGAAGTCCACGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGATTTCGAGGAATTCTTCGAAACCGACTTTTCCTTCGAGGTGCCCGGCGTGGCCCGC
TTCCGGGTCAACGCTTTCAACCACAACCGGGGTGCCGGCGCCGTATTCCGGACCATTCCCTCGAAAGTGCTTACCATGGA
AGACCTGGGCATGGGCAAGGTATTCCAGAAAATCTCCGACATGCCCCGGGGCATTGTGCTGGTTACCGGGCCCACCGGTT
CCGGTAAGTCCACCACCCTTGCGGCCATGGTCGACTACATCAACGATTCACGCTACGAGCACATCCTCACCATTGAGGAT
CCCATCGAATTCGTGCACGAATCGAAAAAATGCCTGGTCAACCAGCGGGAAGTCCACCGCGATACCCTCGGCTTTTCCGA
AGCCCTGCGATCCGCACTGCGGGAAGACCCGGATATCATTCTGGTGGGCGAGCTGCGGGATCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGTCACCTGGTATTCGGCACCCTGCACACCACCTCCGCTGCCAAGACCATTGACCGGGTG
GTGGATGTATTCCCCGCCGAAGAAAAATCCATGATCCGCTCCATGCTGTCGGAATCCCTGCAGGCGGTGGTATCCCAGAC
CCTGCTGAAGAAAAACGGCGGCGGCCGGGTGGCCGCCCACGAAATCATGATGGGCACACCGGCGATCCGTAACCTGATCC
GCGAAGACAAGGTGGCGCAGATGTACTCGGCCATCCAGACCGGCGGTGCGCTGGGCATGCAGACCCTGGATCAGTGCCTG
CAGGGTCTGGTCAATAAAGGCCTCGTGGCCCGCGATGTGGCCCGCGAAAAAGCCAAGAGCCCGGACTCAATCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2D5DPV3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

89.535

100

0.895
  pilT Pseudomonas aeruginosa PAK

88.663

100

0.887
  pilT Acinetobacter nosocomialis M2

83.673

99.709

0.834
  pilT Acinetobacter baumannii D1279779

83.673

99.709

0.834
  pilT Acinetobacter baumannii strain A118

83.673

99.709

0.834
  pilT Acinetobacter baylyi ADP1

81.341

99.709

0.811
  pilT Legionella pneumophila strain Lp02

77.219

98.256

0.759
  pilT Legionella pneumophila strain ERS1305867

77.219

98.256

0.759
  pilT Neisseria meningitidis 8013

68.116

100

0.683
  pilT Neisseria gonorrhoeae MS11

67.536

100

0.677
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.301

95.64

0.663
  pilT Vibrio cholerae strain A1552

69.301

95.64

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.754

99.419

0.515
  pilU Vibrio cholerae strain A1552

40.29

100

0.404
  pilU Pseudomonas stutzeri DSM 10701

39.71

100

0.398
  pilU Acinetobacter baylyi ADP1

38.439

100

0.387

Multiple sequence alignment