Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACMG4L_RS12620 Genome accession   NZ_CP179933
Coordinates   2709207..2710328 (-) Length   373 a.a.
NCBI ID   WP_035249738.1    Uniprot ID   -
Organism   Alcanivorax sp. IL1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2704207..2715328
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMG4L_RS12590 (ACMG4L_12590) arsH 2704901..2705611 (+) 711 WP_035249726.1 arsenical resistance protein ArsH -
  ACMG4L_RS12595 (ACMG4L_12595) - 2705758..2706678 (-) 921 WP_414430505.1 glutathione S-transferase family protein -
  ACMG4L_RS12600 (ACMG4L_12600) - 2706748..2707302 (-) 555 WP_414430504.1 YaeQ family protein -
  ACMG4L_RS12605 (ACMG4L_12605) - 2707397..2707948 (-) 552 WP_035249732.1 TMEM165/GDT1 family protein -
  ACMG4L_RS12610 (ACMG4L_12610) - 2708296..2708505 (+) 210 WP_035249734.1 SlyX family protein -
  ACMG4L_RS12615 (ACMG4L_12615) - 2708661..2709086 (-) 426 WP_035249736.1 TM2 domain-containing protein -
  ACMG4L_RS12620 (ACMG4L_12620) pilU 2709207..2710328 (-) 1122 WP_035249738.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACMG4L_RS12625 (ACMG4L_12625) pilT 2710344..2711378 (-) 1035 WP_272962781.1 type IV pilus twitching motility protein PilT Machinery gene
  ACMG4L_RS12630 (ACMG4L_12630) - 2711515..2712198 (+) 684 WP_414430503.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACMG4L_RS12635 (ACMG4L_12635) proC 2712272..2713081 (+) 810 WP_414430502.1 pyrroline-5-carboxylate reductase -
  ACMG4L_RS12640 (ACMG4L_12640) - 2713378..2713926 (+) 549 WP_272962778.1 YggT family protein -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41453.87 Da        Isoelectric Point: 6.7783

>NTDB_id=1093987 ACMG4L_RS12620 WP_035249738.1 2709207..2710328(-) (pilU) [Alcanivorax sp. IL1]
MEFEELLKLMVQKGGSDLFITAGVPPSMKVNGKILPVTKSALTPDMTRDIVFGVMTEQQRKEFVAHHECNFAISARGIGR
FRVSAFYQRNLVAMVLRRIETKIPNVDDLRLPPVIKELAMTKRGLVIFVGATGTGKSTSLASMIGYRNKNSKGHIITIED
PIEFIHQHQGCIVTQREVGIDTESFDVALKNTLRQAPDVILIGEIRTPETMDKAIAFAETGHLCLATLHANNANQALDRI
IHFFPADRHGQLWMDLSLNLKGVVAQQLIPTPDGKGRRAAIEVLLGTPLVQDHIRKGEVHMLKQLMSKSREQGMQTFDQA
LYDLYSVGEITYEDAMLHADSPNDLRLMIKLGNETDSSSLDIATRGLSIEDTE

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=1093987 ACMG4L_RS12620 WP_035249738.1 2709207..2710328(-) (pilU) [Alcanivorax sp. IL1]
ATGGAATTCGAAGAGCTCCTCAAACTGATGGTACAGAAAGGCGGCTCCGACCTGTTCATTACTGCCGGTGTGCCGCCCAG
CATGAAGGTGAACGGCAAGATCCTGCCTGTGACCAAGAGCGCGCTGACCCCGGACATGACCCGGGACATCGTGTTCGGCG
TAATGACCGAACAGCAGCGCAAGGAATTCGTGGCACACCACGAGTGCAACTTCGCCATCTCCGCCCGGGGCATCGGCCGG
TTCCGGGTATCCGCTTTCTACCAGCGCAACCTGGTGGCCATGGTGCTGCGTCGTATCGAAACCAAGATCCCCAATGTGGA
CGACCTGCGTCTGCCGCCAGTGATCAAGGAACTGGCCATGACCAAGCGCGGCCTGGTGATCTTCGTGGGCGCCACCGGTA
CCGGTAAATCCACCTCGCTGGCCTCCATGATCGGCTATCGCAACAAGAACTCGAAGGGCCACATCATCACCATTGAGGAC
CCCATCGAATTCATCCATCAGCATCAGGGTTGTATCGTTACCCAGCGTGAAGTGGGCATTGATACCGAGTCCTTTGATGT
AGCGCTGAAGAACACCCTGCGTCAAGCGCCGGACGTGATCCTGATCGGCGAGATCCGTACCCCGGAAACCATGGACAAGG
CCATTGCCTTCGCCGAAACCGGTCACCTCTGCCTGGCTACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGGATC
ATCCATTTCTTTCCGGCGGACCGCCACGGCCAGCTGTGGATGGACCTGTCCCTGAACCTGAAGGGCGTCGTCGCCCAGCA
GCTGATCCCCACCCCGGACGGCAAGGGCCGCCGCGCCGCCATCGAGGTACTGCTGGGCACGCCGCTGGTTCAGGACCACA
TCCGCAAGGGCGAAGTGCACATGCTCAAGCAGCTGATGAGCAAGTCCCGGGAACAGGGCATGCAGACTTTCGACCAGGCC
CTCTACGACCTCTACTCCGTTGGTGAAATCACCTACGAAGACGCCATGCTGCACGCGGACTCACCCAATGACCTGCGTCT
GATGATCAAGCTGGGTAACGAGACTGATTCCTCCAGCCTGGACATCGCCACGCGGGGATTGAGTATCGAGGACACTGAGT
AA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

79.73

99.196

0.791
  pilU Acinetobacter baylyi ADP1

67.787

95.71

0.649
  pilU Vibrio cholerae strain A1552

55.525

97.051

0.539
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.739

92.493

0.386
  pilT Legionella pneumophila strain Lp02

41.493

89.812

0.373
  pilT Legionella pneumophila strain ERS1305867

41.493

89.812

0.373

Multiple sequence alignment