Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACMG4N_RS03210 Genome accession   NZ_CP179932
Coordinates   705452..706486 (-) Length   344 a.a.
NCBI ID   WP_297767892.1    Uniprot ID   -
Organism   Alcanivorax sp. IL2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 700452..711486
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMG4N_RS03175 (ACMG4N_03175) - 700530..701450 (-) 921 WP_297767874.1 glutathione S-transferase family protein -
  ACMG4N_RS03180 (ACMG4N_03180) - 701520..702074 (-) 555 WP_297768095.1 YaeQ family protein -
  ACMG4N_RS03185 (ACMG4N_03185) - 702175..702726 (-) 552 WP_297767877.1 TMEM165/GDT1 family protein -
  ACMG4N_RS03190 (ACMG4N_03190) - 703078..703287 (+) 210 WP_035249734.1 SlyX family protein -
  ACMG4N_RS03195 (ACMG4N_03195) - 703281..703676 (-) 396 WP_414432890.1 hypothetical protein -
  ACMG4N_RS03200 (ACMG4N_03200) - 703754..704194 (-) 441 WP_414432891.1 TM2 domain-containing protein -
  ACMG4N_RS03205 (ACMG4N_03205) pilU 704316..705437 (-) 1122 WP_297767888.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACMG4N_RS03210 (ACMG4N_03210) pilT 705452..706486 (-) 1035 WP_297767892.1 type IV pilus twitching motility protein PilT Machinery gene
  ACMG4N_RS03215 (ACMG4N_03215) - 706623..707306 (+) 684 WP_414432892.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACMG4N_RS03220 (ACMG4N_03220) proC 707379..708188 (+) 810 WP_297767898.1 pyrroline-5-carboxylate reductase -
  ACMG4N_RS03225 (ACMG4N_03225) - 708402..708950 (+) 549 WP_297767901.1 YggT family protein -
  ACMG4N_RS03230 (ACMG4N_03230) - 709082..710554 (+) 1473 WP_297767903.1 PAS domain S-box protein -
  ACMG4N_RS03235 (ACMG4N_03235) - 710818..711255 (+) 438 WP_297767905.1 PaaI family thioesterase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37935.60 Da        Isoelectric Point: 6.3485

>NTDB_id=1093949 ACMG4N_RS03210 WP_297767892.1 705452..706486(-) (pilT) [Alcanivorax sp. IL2]
MDITELLAFSAKNGASDLHLSAGLPPMIRVDGDVRRINLPAMEHKEVHALIYDIMNDKQRKDFEEFFETDFSFEVPGVAR
FRVNAFNHNRGAGAVFRTIPSKVLTMEDLGMGKVFQKISDMPRGIVLVTGPTGSGKSTTLAAMVDYINDSRYEHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMIRSMLSESLQAVVSQTLMKKNGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGGALGMQTLDQCL
QGLVNKGLVARDIAREKAKSPDSI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1093949 ACMG4N_RS03210 WP_297767892.1 705452..706486(-) (pilT) [Alcanivorax sp. IL2]
ATGGATATTACCGAGCTGCTCGCTTTTAGCGCCAAAAACGGCGCGTCCGATCTTCATCTGTCCGCCGGTCTGCCTCCGAT
GATCCGCGTGGACGGCGACGTGCGCCGCATCAATCTGCCGGCCATGGAGCACAAGGAAGTCCACGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGATTTCGAAGAATTCTTCGAAACGGATTTTTCCTTCGAGGTGCCCGGCGTGGCCCGT
TTCCGGGTGAATGCCTTCAACCACAACCGCGGTGCCGGTGCGGTATTCCGGACCATTCCCTCGAAAGTACTAACCATGGA
AGACCTGGGCATGGGCAAGGTCTTCCAGAAAATCTCCGACATGCCCCGGGGCATTGTGCTGGTTACCGGCCCCACCGGTT
CCGGTAAGTCCACCACCCTGGCGGCCATGGTCGACTACATCAACGATTCGCGCTACGAGCACATCCTCACCATTGAGGAT
CCCATCGAATTCGTGCACGAATCGAAAAAATGCCTGGTCAACCAGCGGGAAGTCCACCGCGATACCCTGGGCTTTTCCGA
AGCCTTGCGCTCGGCCCTGCGGGAGGATCCGGATATTATTCTGGTGGGCGAACTGCGGGATCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGTCACCTGGTATTCGGCACCCTGCACACCACCTCCGCTGCCAAGACCATTGACCGGGTG
GTGGATGTTTTCCCCGCCGAAGAAAAATCCATGATCCGTTCCATGCTGTCGGAATCCCTGCAGGCGGTGGTATCCCAGAC
CCTGATGAAGAAAAACGGTGGCGGCCGGGTGGCCGCCCACGAAATCATGATGGGCACTCCGGCGATCCGTAACCTGATCC
GCGAAGACAAGGTGGCGCAGATGTACTCTGCCATCCAGACCGGCGGCGCGCTGGGCATGCAGACACTGGATCAGTGCCTG
CAGGGGCTGGTCAATAAAGGCCTCGTGGCCCGCGACATAGCCCGGGAAAAAGCCAAGAGCCCGGACTCAATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

89.244

100

0.892
  pilT Pseudomonas aeruginosa PAK

88.663

100

0.887
  pilT Acinetobacter nosocomialis M2

83.382

99.709

0.831
  pilT Acinetobacter baumannii D1279779

83.382

99.709

0.831
  pilT Acinetobacter baumannii strain A118

83.382

99.709

0.831
  pilT Acinetobacter baylyi ADP1

81.05

99.709

0.808
  pilT Legionella pneumophila strain Lp02

76.923

98.256

0.756
  pilT Legionella pneumophila strain ERS1305867

76.923

98.256

0.756
  pilT Neisseria meningitidis 8013

67.536

100

0.677
  pilT Neisseria gonorrhoeae MS11

67.246

100

0.674
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.997

95.64

0.66
  pilT Vibrio cholerae strain A1552

68.997

95.64

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.462

99.419

0.512
  pilU Vibrio cholerae strain A1552

40

100

0.401
  pilU Pseudomonas stutzeri DSM 10701

39.71

100

0.398
  pilU Acinetobacter baylyi ADP1

38.439

100

0.387

Multiple sequence alignment