Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACMG4N_RS03205 Genome accession   NZ_CP179932
Coordinates   704316..705437 (-) Length   373 a.a.
NCBI ID   WP_297767888.1    Uniprot ID   -
Organism   Alcanivorax sp. IL2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 699316..710437
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMG4N_RS03175 (ACMG4N_03175) - 700530..701450 (-) 921 WP_297767874.1 glutathione S-transferase family protein -
  ACMG4N_RS03180 (ACMG4N_03180) - 701520..702074 (-) 555 WP_297768095.1 YaeQ family protein -
  ACMG4N_RS03185 (ACMG4N_03185) - 702175..702726 (-) 552 WP_297767877.1 TMEM165/GDT1 family protein -
  ACMG4N_RS03190 (ACMG4N_03190) - 703078..703287 (+) 210 WP_035249734.1 SlyX family protein -
  ACMG4N_RS03195 (ACMG4N_03195) - 703281..703676 (-) 396 WP_414432890.1 hypothetical protein -
  ACMG4N_RS03200 (ACMG4N_03200) - 703754..704194 (-) 441 WP_414432891.1 TM2 domain-containing protein -
  ACMG4N_RS03205 (ACMG4N_03205) pilU 704316..705437 (-) 1122 WP_297767888.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACMG4N_RS03210 (ACMG4N_03210) pilT 705452..706486 (-) 1035 WP_297767892.1 type IV pilus twitching motility protein PilT Machinery gene
  ACMG4N_RS03215 (ACMG4N_03215) - 706623..707306 (+) 684 WP_414432892.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACMG4N_RS03220 (ACMG4N_03220) proC 707379..708188 (+) 810 WP_297767898.1 pyrroline-5-carboxylate reductase -
  ACMG4N_RS03225 (ACMG4N_03225) - 708402..708950 (+) 549 WP_297767901.1 YggT family protein -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41411.83 Da        Isoelectric Point: 6.8257

>NTDB_id=1093948 ACMG4N_RS03205 WP_297767888.1 704316..705437(-) (pilU) [Alcanivorax sp. IL2]
MEFEELLKLMVQKGGSDLFITAGVPPSMKVNGKILPVTKSALTPDMTRDIVFGVMTEQQRKEFVAHHECNFAISARGIGR
FRVSAFYQRNLVAMVLRRIETKIPNVDDLRLPPVIKELAMTKRGLVIFVGATGTGKSTSLASMIGHRNKNSKGHIITIED
PIEFIHQHQGCIVTQREVGIDTESFDVALKNTLRQAPDVILIGEIRTPDTMDKAIAFAETGHLCLATLHANNANQALDRI
IHFFPADRHGQLWMDLSLNLKGIVAQQLIPTPDGKGRRAAIEVLLGTPLVQDHIRKGEVHMLKQLMSKSREQGMQTFDQA
LYDLYSVGEITYEDAMLHADSPNDLRLMIKLGNETDASSLDIATRGLSIEDTE

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=1093948 ACMG4N_RS03205 WP_297767888.1 704316..705437(-) (pilU) [Alcanivorax sp. IL2]
ATGGAATTCGAAGAGCTCCTCAAACTGATGGTACAGAAAGGCGGCTCCGACCTGTTCATCACCGCCGGGGTTCCACCCAG
CATGAAGGTGAACGGCAAGATCCTGCCGGTGACCAAGAGCGCGCTGACCCCGGACATGACCCGGGACATCGTGTTTGGCG
TGATGACCGAACAGCAACGCAAGGAATTCGTCGCCCACCACGAGTGCAACTTCGCCATTTCCGCCCGGGGCATCGGCCGT
TTCCGGGTGTCCGCCTTCTACCAACGCAACCTGGTGGCCATGGTGCTGCGCCGCATCGAGACCAAGATCCCCAACGTGGA
TGACCTGCGTCTGCCGCCAGTGATCAAGGAACTGGCCATGACCAAGCGGGGCCTGGTGATCTTCGTGGGGGCCACCGGTA
CTGGTAAGTCCACCTCGTTGGCCTCCATGATCGGCCACCGCAACAAGAACTCGAAGGGCCACATCATCACCATTGAGGAC
CCCATCGAATTCATCCACCAGCACCAGGGGTGCATCGTCACCCAGCGTGAGGTGGGTATCGATACCGAGTCCTTTGATGT
AGCGCTGAAGAACACCCTGCGTCAGGCGCCGGATGTGATCCTGATCGGTGAGATCCGTACCCCGGACACCATGGACAAGG
CCATCGCCTTCGCGGAAACCGGCCACCTGTGTCTGGCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGGATC
ATCCACTTCTTCCCGGCAGATCGTCACGGCCAGCTGTGGATGGATCTGTCCCTGAACCTGAAAGGCATCGTCGCCCAGCA
ACTGATTCCCACCCCGGACGGCAAGGGTCGCCGCGCTGCCATCGAGGTACTGCTGGGCACACCACTGGTGCAGGACCATA
TCCGCAAGGGCGAAGTGCACATGCTCAAGCAGCTGATGAGCAAGTCCCGGGAACAGGGTATGCAGACCTTCGACCAGGCC
CTTTACGACCTCTACTCCGTGGGCGAGATCACCTACGAAGACGCCATGCTGCACGCGGATTCCCCCAATGATCTGCGCCT
GATGATCAAGCTCGGCAACGAAACCGATGCCTCCAGCCTGGATATCGCCACGCGAGGGTTGAGCATCGAGGACACCGAGT
AA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

79.459

99.196

0.788
  pilU Acinetobacter baylyi ADP1

67.507

95.71

0.646
  pilU Vibrio cholerae strain A1552

54.596

96.247

0.525
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.896

95.71

0.391
  pilT Legionella pneumophila strain Lp02

40.597

89.812

0.365
  pilT Legionella pneumophila strain ERS1305867

40.597

89.812

0.365

Multiple sequence alignment