Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACLUPT_RS28725 Genome accession   NZ_CP179718
Coordinates   6205917..6206960 (+) Length   347 a.a.
NCBI ID   WP_237676343.1    Uniprot ID   -
Organism   Variovorax sp. SCN45     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6200917..6211960
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLUPT_RS28695 (ACLUPT_28695) - 6201214..6202020 (+) 807 WP_237676337.1 ABC transporter ATP-binding protein -
  ACLUPT_RS28700 (ACLUPT_28700) dapA 6202057..6202950 (+) 894 WP_237676338.1 4-hydroxy-tetrahydrodipicolinate synthase -
  ACLUPT_RS28705 (ACLUPT_28705) - 6202958..6203800 (+) 843 WP_237676339.1 alpha/beta hydrolase -
  ACLUPT_RS28710 (ACLUPT_28710) - 6203797..6204387 (+) 591 WP_237676340.1 malonic semialdehyde reductase -
  ACLUPT_RS28715 (ACLUPT_28715) - 6204398..6205138 (+) 741 WP_237676341.1 sulfite exporter TauE/SafE family protein -
  ACLUPT_RS28720 (ACLUPT_28720) - 6205164..6205865 (-) 702 WP_237676342.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACLUPT_RS28725 (ACLUPT_28725) pilT 6205917..6206960 (+) 1044 WP_237676343.1 type IV pilus twitching motility protein PilT Machinery gene
  ACLUPT_RS28730 (ACLUPT_28730) pilU 6206993..6208129 (+) 1137 WP_237676344.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACLUPT_RS28735 (ACLUPT_28735) - 6208126..6209676 (-) 1551 WP_237676345.1 MFS transporter -
  ACLUPT_RS28740 (ACLUPT_28740) - 6209673..6210155 (-) 483 WP_237676346.1 MerR family transcriptional regulator -
  ACLUPT_RS28745 (ACLUPT_28745) - 6210243..6211151 (+) 909 WP_237676347.1 NAD(P)-dependent oxidoreductase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38205.88 Da        Isoelectric Point: 6.8356

>NTDB_id=1092816 ACLUPT_RS28725 WP_237676343.1 6205917..6206960(+) (pilT) [Variovorax sp. SCN45]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVNGDVRRINVDALDHKGVHAMVYDIMSDTHRKHYEEFLEVDFSFEIDGLAR
FRVNAFNQARGAAAVFRTIPSKILTLEQLNAPKIFGELALKPRGLVLVTGPTGSGKSTTLAAMVNYLNENEYGHILTVED
PIEFVHESKKCLINQREVGPMTLSFSNALRSALREDPDAILVGELRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGEEKEMIRAMLSESLQAVISQTLCKTKDGQSRVAAHEIMLGTPAIRNLIREAKVAQMYSTIQTGQGQGMQTLDQN
LTELVRRNTISAAEARGKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1092816 ACLUPT_RS28725 WP_237676343.1 6205917..6206960(+) (pilT) [Variovorax sp. SCN45]
GTGGACATCACCCAACTGCTGGCATTCAGCGTCAAGAACAAAGCCTCCGACCTGCATCTTTCCGCGGGCCTGCCGCCCAT
GATCCGCGTAAACGGCGACGTGCGCCGCATCAACGTCGACGCGCTCGACCACAAGGGCGTGCATGCGATGGTGTACGACA
TCATGAGCGACACGCACCGCAAGCACTACGAAGAGTTCCTGGAGGTCGACTTCTCGTTCGAGATCGACGGCCTCGCGCGC
TTCCGCGTGAACGCCTTCAACCAGGCGCGCGGCGCCGCCGCGGTGTTCCGGACCATTCCCTCGAAGATCCTCACGCTGGA
GCAGCTGAACGCGCCCAAGATTTTCGGCGAACTGGCGCTCAAGCCGCGCGGGCTGGTTCTGGTGACGGGCCCCACGGGCT
CTGGCAAGTCGACCACGCTGGCCGCGATGGTCAACTATCTCAACGAAAACGAATACGGCCACATCCTCACGGTGGAAGAC
CCGATCGAATTCGTGCACGAGTCGAAGAAGTGCCTGATCAACCAGCGCGAAGTGGGCCCGATGACGCTGTCGTTCTCGAA
CGCGCTGCGCTCCGCCCTGCGCGAAGACCCGGACGCCATCCTGGTGGGCGAGTTGCGCGACCTAGAAACCATCCGCCTGG
CCATGACCGCGGCCGAAACGGGCCACCTGGTGTTCGGCACGCTGCATACCTCGTCGGCCGCCAAGACCATCGACCGGATC
ATCGACGTGTTCCCGGGCGAAGAAAAGGAAATGATCCGCGCGATGCTGTCGGAGTCGCTGCAGGCGGTGATCTCGCAGAC
GCTGTGCAAGACCAAGGACGGCCAGAGCCGCGTGGCGGCGCACGAGATCATGCTGGGCACGCCGGCCATCCGCAACCTGA
TCCGCGAGGCCAAGGTGGCGCAGATGTACTCCACCATCCAGACCGGCCAGGGCCAGGGCATGCAGACGCTCGACCAGAAC
CTGACGGAACTGGTGCGGCGCAATACCATCTCGGCTGCGGAGGCCAGAGGCAAGGCCAAGATCCCCGAAAACTTCCCCGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

75.652

99.424

0.752
  pilT Acinetobacter nosocomialis M2

75.362

99.424

0.749
  pilT Acinetobacter baumannii D1279779

75.362

99.424

0.749
  pilT Acinetobacter baumannii strain A118

75.362

99.424

0.749
  pilT Pseudomonas stutzeri DSM 10701

75.072

99.424

0.746
  pilT Acinetobacter baylyi ADP1

73.913

99.424

0.735
  pilT Legionella pneumophila strain Lp02

73.746

97.695

0.72
  pilT Legionella pneumophila strain ERS1305867

73.746

97.695

0.72
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.622

98.271

0.674
  pilT Vibrio cholerae strain A1552

68.622

98.271

0.674
  pilT Neisseria meningitidis 8013

66.957

99.424

0.666
  pilT Neisseria gonorrhoeae MS11

66.667

99.424

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.145

99.424

0.499
  pilU Vibrio cholerae strain A1552

43.881

96.542

0.424
  pilU Acinetobacter baylyi ADP1

41.329

99.712

0.412
  pilU Pseudomonas stutzeri DSM 10701

40.896

96.542

0.395

Multiple sequence alignment