Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACLUPT_RS28730 Genome accession   NZ_CP179718
Coordinates   6206993..6208129 (+) Length   378 a.a.
NCBI ID   WP_237676344.1    Uniprot ID   -
Organism   Variovorax sp. SCN45     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6201993..6213129
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLUPT_RS28700 (ACLUPT_28700) dapA 6202057..6202950 (+) 894 WP_237676338.1 4-hydroxy-tetrahydrodipicolinate synthase -
  ACLUPT_RS28705 (ACLUPT_28705) - 6202958..6203800 (+) 843 WP_237676339.1 alpha/beta hydrolase -
  ACLUPT_RS28710 (ACLUPT_28710) - 6203797..6204387 (+) 591 WP_237676340.1 malonic semialdehyde reductase -
  ACLUPT_RS28715 (ACLUPT_28715) - 6204398..6205138 (+) 741 WP_237676341.1 sulfite exporter TauE/SafE family protein -
  ACLUPT_RS28720 (ACLUPT_28720) - 6205164..6205865 (-) 702 WP_237676342.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACLUPT_RS28725 (ACLUPT_28725) pilT 6205917..6206960 (+) 1044 WP_237676343.1 type IV pilus twitching motility protein PilT Machinery gene
  ACLUPT_RS28730 (ACLUPT_28730) pilU 6206993..6208129 (+) 1137 WP_237676344.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACLUPT_RS28735 (ACLUPT_28735) - 6208126..6209676 (-) 1551 WP_237676345.1 MFS transporter -
  ACLUPT_RS28740 (ACLUPT_28740) - 6209673..6210155 (-) 483 WP_237676346.1 MerR family transcriptional regulator -
  ACLUPT_RS28745 (ACLUPT_28745) - 6210243..6211151 (+) 909 WP_237676347.1 NAD(P)-dependent oxidoreductase -
  ACLUPT_RS28750 (ACLUPT_28750) - 6211181..6211993 (-) 813 WP_237676348.1 BON domain-containing protein -
  ACLUPT_RS28755 (ACLUPT_28755) - 6211990..6212634 (-) 645 WP_237676349.1 SIS domain-containing protein -
  ACLUPT_RS28760 (ACLUPT_28760) - 6212720..6213088 (-) 369 WP_237676350.1 YraN family protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41875.00 Da        Isoelectric Point: 6.2432

>NTDB_id=1092817 ACLUPT_RS28730 WP_237676344.1 6206993..6208129(+) (pilU) [Variovorax sp. SCN45]
MERDQASQFINDLLKLMVSRAGSDLFITADFPPAIKVDGKVTKVSQQALGAQHTLALTRSIMNDRQTAEFERTKECNFAI
SPTGIGRFRVNAFVQQGKVGMVLRTIPAKLPTIDDLGMPQVLKEVTMSKRGLCILVGATGSGKSTTLAAMIDWRNENSFG
HIVTVEDPVEFVHPHKNCVVTQREVGIDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCMATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRSLVSQRLVPTEDGLGRVAAFEVLLNTPLISDLIFKGEVGEIKEIMKKSRNLG
MQTFDQALFDLFESHSITFEDAIRNADSANDLRLQIKLNSQRARSSDLAAGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=1092817 ACLUPT_RS28730 WP_237676344.1 6206993..6208129(+) (pilU) [Variovorax sp. SCN45]
ATGGAACGCGATCAAGCCAGCCAGTTCATCAACGACCTCCTGAAGCTCATGGTGAGCCGCGCCGGCAGCGACCTGTTCAT
CACCGCCGACTTCCCGCCGGCCATCAAGGTCGACGGCAAGGTGACCAAGGTGTCGCAGCAGGCGCTGGGCGCGCAGCACA
CGCTGGCGCTCACGCGCTCGATCATGAACGACCGCCAGACGGCGGAATTCGAGCGCACCAAGGAGTGCAACTTCGCGATC
TCGCCGACCGGCATCGGCCGCTTCCGCGTGAACGCGTTCGTGCAGCAGGGCAAGGTCGGCATGGTGCTGCGGACCATTCC
CGCCAAGCTGCCGACCATCGACGACCTGGGCATGCCGCAGGTGCTCAAGGAAGTGACGATGAGCAAGCGCGGCCTGTGCA
TCCTGGTGGGTGCCACCGGCTCGGGCAAGTCGACCACGCTGGCCGCGATGATCGACTGGCGCAACGAAAATTCGTTCGGC
CACATCGTGACGGTGGAAGACCCGGTGGAGTTCGTGCACCCGCACAAGAACTGCGTGGTGACGCAGCGCGAAGTGGGCAT
CGACACCGACAGCTGGGAAGCCGCGCTGAAGAACACGCTGCGCCAGGCACCCGACGTGATCCTGATGGGCGAAATCCGCG
ACCGCGAGACCATGGAGCACGCCGTGGCCTTCGCCGAGACCGGCCACCTGTGCATGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCACTGGACCGCATCATCAACTTCTTCCCCGAAGAGCGCCGCGCGCAGCTGCTGATGGACCTGTCGCTGAACCT
GCGCTCGCTGGTGTCGCAGCGGCTGGTGCCAACCGAAGACGGCCTGGGCCGCGTGGCGGCTTTCGAAGTGCTGCTGAACA
CGCCGCTGATTTCCGACCTGATCTTCAAGGGCGAGGTGGGCGAGATCAAGGAAATCATGAAGAAGAGCCGCAACCTGGGC
ATGCAGACCTTCGACCAGGCGCTGTTCGACCTGTTCGAGAGCCACTCGATCACTTTCGAGGACGCGATCCGCAACGCCGA
TTCGGCCAACGACCTGCGGCTGCAGATCAAGCTCAACAGCCAGCGCGCGCGCAGCAGCGACCTCGCTGCCGGCACCGAGC
ACTTCGCGATCGTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(41-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.183

92.328

0.593
  pilU Acinetobacter baylyi ADP1

58.101

94.709

0.55
  pilU Vibrio cholerae strain A1552

53.168

96.032

0.511
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.401

89.153

0.405
  pilT Pseudomonas aeruginosa PAK

44.51

89.153

0.397
  pilT Pseudomonas stutzeri DSM 10701

43.323

89.153

0.386
  pilT Acinetobacter nosocomialis M2

43.544

88.095

0.384
  pilT Legionella pneumophila strain ERS1305867

44.073

87.037

0.384
  pilT Legionella pneumophila strain Lp02

44.073

87.037

0.384
  pilT Acinetobacter baumannii D1279779

44.514

84.392

0.376
  pilT Acinetobacter baumannii strain A118

44.514

84.392

0.376
  pilT Acinetobacter baylyi ADP1

42.042

88.095

0.37

Multiple sequence alignment