Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACJ8W9_RS00150 Genome accession   NZ_CP178751
Coordinates   32212..33249 (+) Length   345 a.a.
NCBI ID   WP_034732218.1    Uniprot ID   A0A094J8M8
Organism   Pseudidiomarina sp. YC-516-91     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 27212..38249
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJ8W9_RS00120 rdgB 27245..27841 (-) 597 WP_411357932.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  ACJ8W9_RS00125 - 27914..29641 (+) 1728 WP_411357933.1 potassium/proton antiporter -
  ACJ8W9_RS00130 - 29638..30078 (-) 441 WP_411357934.1 DUF4426 domain-containing protein -
  ACJ8W9_RS00135 - 30094..30618 (-) 525 WP_411357935.1 YggT family protein -
  ACJ8W9_RS00140 proC 30631..31458 (-) 828 WP_411357936.1 pyrroline-5-carboxylate reductase -
  ACJ8W9_RS00145 - 31462..32160 (-) 699 WP_411357937.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACJ8W9_RS00150 pilT 32212..33249 (+) 1038 WP_034732218.1 type IV pilus twitching motility protein PilT Machinery gene
  ACJ8W9_RS00155 pilU 33282..34394 (+) 1113 WP_411357938.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACJ8W9_RS00160 tsaD 34444..35469 (+) 1026 WP_411357939.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD -
  ACJ8W9_RS00165 plsY 35466..36053 (-) 588 WP_034732215.1 glycerol-3-phosphate 1-O-acyltransferase PlsY -
  ACJ8W9_RS00170 folB 36152..36505 (+) 354 WP_411357940.1 dihydroneopterin aldolase -
  ACJ8W9_RS00175 folK 36507..37058 (+) 552 WP_411357941.1 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine diphosphokinase -
  ACJ8W9_RS00180 - 37055..37849 (+) 795 WP_411357942.1 undecaprenyl-diphosphate phosphatase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38293.90 Da        Isoelectric Point: 6.8284

>NTDB_id=1091335 ACJ8W9_RS00150 WP_034732218.1 32212..33249(+) (pilT) [Pseudidiomarina sp. YC-516-91]
MDITELLGFAVKHNSSDLHLSADSPPMIRVDGDVRKINIPSLSHKQVQELVYDIMNDRQRKEYEQNLECDFSFEVPDLAR
FRVNVFVQNRGCGAVLRTIPSEVLTLEQLGAPEIFKTIADQPRGLVLVTGPTGSGKSTTLAAMVDFINESKHHHILTIED
PIEFVHRNKQSLVNQREVHRDTHSFANALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAPKTIDRI
IDVFPGDEKPMVRSMLSESLRAVISQTLLKRVGGGRVAAHEIMIATPAIKNLIREDKVAQMYSSIQTGAAHGMQTLDQGL
LNLLNQGLVSRLEARAKAANKEAFK

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1091335 ACJ8W9_RS00150 WP_034732218.1 32212..33249(+) (pilT) [Pseudidiomarina sp. YC-516-91]
ATGGATATTACTGAGTTACTGGGCTTCGCCGTTAAACATAACTCGTCGGACTTACACTTATCAGCGGATTCACCGCCTAT
GATACGTGTCGATGGGGATGTTCGTAAAATCAATATTCCATCTTTGTCGCACAAACAGGTGCAAGAGTTGGTTTACGACA
TCATGAATGACCGGCAGCGGAAAGAATACGAACAAAATTTGGAATGCGACTTCTCATTTGAAGTTCCTGATTTAGCGCGC
TTCCGGGTCAATGTTTTTGTCCAAAATAGAGGCTGTGGTGCGGTATTACGTACCATTCCGAGTGAAGTTTTAACGCTAGA
GCAGTTGGGTGCGCCAGAAATTTTCAAAACCATTGCCGATCAACCTCGAGGGTTGGTGTTAGTGACGGGGCCAACTGGCT
CGGGTAAGTCAACCACTCTCGCCGCGATGGTGGATTTCATCAATGAAAGTAAGCATCACCATATACTGACCATCGAAGAC
CCGATCGAATTTGTGCACCGTAACAAGCAGAGCTTGGTCAACCAACGTGAAGTGCATCGTGACACTCATAGCTTCGCAAA
TGCACTACGCTCTGCTCTGCGGGAAGACCCTGATGTCATTCTGGTGGGTGAGATGCGTGACCTAGAAACCATTCGCTTGG
CGATGACTGCAGCCGAGACGGGCCACTTGGTGTTTGGTACATTGCACACCACCTCGGCACCCAAAACTATAGACCGTATC
ATCGACGTGTTCCCCGGTGACGAAAAACCGATGGTTCGTTCCATGCTTTCAGAATCCTTACGTGCGGTTATTTCACAAAC
GCTGTTGAAGCGTGTTGGTGGCGGACGAGTCGCAGCGCACGAAATCATGATCGCAACTCCAGCTATCAAAAATCTCATTC
GTGAAGACAAGGTCGCGCAGATGTACTCATCGATTCAGACGGGTGCTGCGCACGGTATGCAGACTCTTGACCAAGGTCTG
CTAAACTTATTGAATCAAGGCTTGGTTTCGCGTCTCGAGGCGCGCGCCAAAGCTGCTAACAAAGAGGCGTTCAAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A094J8M8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

75.872

99.71

0.757
  pilT Pseudomonas aeruginosa PAK

75.291

99.71

0.751
  pilT Acinetobacter nosocomialis M2

74.709

99.71

0.745
  pilT Acinetobacter baumannii D1279779

74.709

99.71

0.745
  pilT Acinetobacter baumannii strain A118

74.709

99.71

0.745
  pilT Acinetobacter baylyi ADP1

74.709

99.71

0.745
  pilT Legionella pneumophila strain Lp02

71.802

99.71

0.716
  pilT Legionella pneumophila strain ERS1305867

71.802

99.71

0.716
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.7

97.681

0.71
  pilT Vibrio cholerae strain A1552

72.7

97.681

0.71
  pilT Neisseria meningitidis 8013

67.246

100

0.672
  pilT Neisseria gonorrhoeae MS11

66.957

100

0.67
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.464

100

0.525
  pilU Pseudomonas stutzeri DSM 10701

42.09

97.101

0.409
  pilU Vibrio cholerae strain A1552

40.816

99.42

0.406
  pilU Acinetobacter baylyi ADP1

38.393

97.391

0.374

Multiple sequence alignment