Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACJ8W9_RS00155 Genome accession   NZ_CP178751
Coordinates   33282..34394 (+) Length   370 a.a.
NCBI ID   WP_411357938.1    Uniprot ID   -
Organism   Pseudidiomarina sp. YC-516-91     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 28282..39394
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJ8W9_RS00130 - 29638..30078 (-) 441 WP_411357934.1 DUF4426 domain-containing protein -
  ACJ8W9_RS00135 - 30094..30618 (-) 525 WP_411357935.1 YggT family protein -
  ACJ8W9_RS00140 proC 30631..31458 (-) 828 WP_411357936.1 pyrroline-5-carboxylate reductase -
  ACJ8W9_RS00145 - 31462..32160 (-) 699 WP_411357937.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACJ8W9_RS00150 pilT 32212..33249 (+) 1038 WP_034732218.1 type IV pilus twitching motility protein PilT Machinery gene
  ACJ8W9_RS00155 pilU 33282..34394 (+) 1113 WP_411357938.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACJ8W9_RS00160 tsaD 34444..35469 (+) 1026 WP_411357939.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD -
  ACJ8W9_RS00165 plsY 35466..36053 (-) 588 WP_034732215.1 glycerol-3-phosphate 1-O-acyltransferase PlsY -
  ACJ8W9_RS00170 folB 36152..36505 (+) 354 WP_411357940.1 dihydroneopterin aldolase -
  ACJ8W9_RS00175 folK 36507..37058 (+) 552 WP_411357941.1 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine diphosphokinase -
  ACJ8W9_RS00180 - 37055..37849 (+) 795 WP_411357942.1 undecaprenyl-diphosphate phosphatase -
  ACJ8W9_RS00185 - 37829..38944 (-) 1116 WP_411357943.1 hypothetical protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41224.59 Da        Isoelectric Point: 6.5105

>NTDB_id=1091336 ACJ8W9_RS00155 WP_411357938.1 33282..34394(+) (pilU) [Pseudidiomarina sp. YC-516-91]
MLLNKLLQIMIEKNASDLFISANLPPSAKINGELRHLTEQKLSEEGALDLVKDALGEKHHDEYFKDHEANFAIFREGIGR
FRVSAFWQRQRAGMVIRRIVTEIPTVEELKLPEVLTKLIMAKRGLFLVVGGTGTGKSTSLAALLGYRNQHSRGHIITIED
PIEFVHEHGKSMVTQREVGTDTESFESALKSSLRQAPDVILVGEIRSQEVMEYALTFAETGHLCVATLHANNANQAIERI
MHMVPKEKIGKLMFDLSLNLRGIVAQQLIPTVDGGRLAAIEVLLNSPIVAEKIATNDMGEIKEIMAKSRELGMQTFDQAL
FDLYKARKITFEDALRHADAPNDLRLKIKLSGESRPGEEGPLKGVTVDFE

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=1091336 ACJ8W9_RS00155 WP_411357938.1 33282..34394(+) (pilU) [Pseudidiomarina sp. YC-516-91]
ATGTTGCTCAACAAACTACTGCAAATCATGATCGAAAAGAACGCTTCAGATTTGTTTATTTCAGCAAACCTGCCGCCGAG
TGCCAAGATTAATGGTGAGCTGCGGCATTTAACCGAGCAGAAACTGTCTGAAGAAGGCGCCTTAGACTTGGTAAAAGATG
CGCTCGGTGAAAAACATCACGATGAATATTTCAAAGATCACGAAGCGAACTTTGCGATATTCCGTGAAGGTATCGGTCGC
TTCCGGGTCAGTGCATTCTGGCAACGGCAACGCGCCGGTATGGTTATTCGTCGTATCGTCACCGAAATACCCACGGTGGA
AGAACTCAAGTTACCTGAAGTTCTTACCAAGCTGATCATGGCAAAGCGTGGCCTATTTTTGGTTGTGGGTGGTACGGGTA
CCGGTAAGTCGACCAGCTTAGCGGCATTGCTGGGTTACCGAAATCAGCATTCGCGTGGTCACATTATTACCATCGAAGAC
CCGATTGAATTCGTCCATGAGCACGGCAAGTCTATGGTTACCCAGCGTGAAGTTGGTACCGATACGGAGAGTTTTGAATC
CGCACTGAAGAGCTCGTTGCGCCAGGCGCCGGACGTTATTTTGGTCGGTGAGATCCGCTCTCAAGAAGTCATGGAATATG
CGCTCACCTTCGCTGAAACCGGTCATTTATGTGTGGCAACTTTGCACGCCAACAACGCGAACCAAGCGATTGAGCGGATC
ATGCACATGGTGCCGAAAGAGAAAATCGGCAAATTGATGTTCGACTTGTCGTTGAACTTGCGCGGTATTGTGGCGCAGCA
ATTGATTCCGACCGTTGACGGTGGGCGCTTAGCTGCCATTGAAGTATTGCTCAATAGCCCTATCGTGGCGGAGAAAATTG
CCACCAACGATATGGGTGAAATAAAAGAGATTATGGCGAAGTCGCGAGAACTGGGTATGCAAACCTTCGACCAAGCCCTT
TTTGACCTTTACAAAGCTCGCAAAATCACCTTTGAAGACGCATTGCGTCATGCTGATGCACCAAACGATTTACGCTTGAA
AATTAAACTCAGCGGCGAGAGTCGTCCCGGTGAAGAAGGCCCACTCAAAGGTGTGACTGTAGATTTCGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

57.412

100

0.576
  pilU Pseudomonas stutzeri DSM 10701

59.437

95.946

0.57
  pilU Acinetobacter baylyi ADP1

58.146

96.216

0.559
  pilT Pseudomonas aeruginosa PAK

39.826

92.973

0.37
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.65

92.703

0.368
  pilT Vibrio cholerae strain A1552

42.453

85.946

0.365
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.453

85.946

0.365
  pilT Pseudomonas stutzeri DSM 10701

38.953

92.973

0.362

Multiple sequence alignment