Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACLKMI_RS10020 Genome accession   NZ_CP178625
Coordinates   2089325..2090365 (-) Length   346 a.a.
NCBI ID   WP_010391590.1    Uniprot ID   A0A317FVK0
Organism   Pseudoalteromonas sp. KJ71-7     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2084325..2095365
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLKMI_RS09990 (ACLKMI_09990) - 2084528..2085001 (+) 474 WP_010391582.1 hypothetical protein -
  ACLKMI_RS09995 (ACLKMI_09995) rsmE 2085072..2085806 (+) 735 WP_413400781.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACLKMI_RS10000 (ACLKMI_10000) gshB 2085873..2086823 (+) 951 WP_010391584.1 glutathione synthase -
  ACLKMI_RS10005 (ACLKMI_10005) - 2086951..2087508 (+) 558 WP_010391585.1 YqgE/AlgH family protein -
  ACLKMI_RS10010 (ACLKMI_10010) ruvX 2087526..2087975 (+) 450 WP_010391588.1 Holliday junction resolvase RuvX -
  ACLKMI_RS10015 (ACLKMI_10015) pilU 2088179..2089315 (-) 1137 WP_413400782.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACLKMI_RS10020 (ACLKMI_10020) pilT 2089325..2090365 (-) 1041 WP_010391590.1 type IV pilus twitching motility protein PilT Machinery gene
  ACLKMI_RS10025 (ACLKMI_10025) - 2090368..2091081 (+) 714 WP_413400783.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACLKMI_RS10030 (ACLKMI_10030) proC 2091099..2091920 (+) 822 WP_138609266.1 pyrroline-5-carboxylate reductase -
  ACLKMI_RS10035 (ACLKMI_10035) - 2091947..2092480 (+) 534 WP_008113334.1 YggT family protein -
  ACLKMI_RS10040 (ACLKMI_10040) - 2092539..2092982 (+) 444 WP_413400784.1 DUF4426 domain-containing protein -
  ACLKMI_RS10045 (ACLKMI_10045) - 2092985..2093584 (+) 600 WP_413400785.1 XTP/dITP diphosphatase -
  ACLKMI_RS10050 (ACLKMI_10050) hemW 2093581..2094717 (+) 1137 WP_413400786.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38284.52 Da        Isoelectric Point: 6.2312

>NTDB_id=1090667 ACLKMI_RS10020 WP_010391590.1 2089325..2090365(-) (pilT) [Pseudoalteromonas sp. KJ71-7]
MDITELLAFSVQHKASDLHLSSGVSPMIRVDGDVRRINIPALGDKEVSSLVYDIMNDNQRKDYEQNLEVDFSFEVPNLAR
FRVNAFNSNRGPAAVFRTIPSDVLTLEDLGAPEIFKQISDNPRGLVLVTGPTGSGKSTTLAAMVDYINQNKHHHVLTIED
PIEFVHDNKLSLINQREVHRDTHSFSNALRSALREDPDVILVGELRDLETIRLAMTAAETGHLVFGTLHTTSAPKTIDRI
IDVFPGEEKDMIRSMLSESLRAVISQTLIKKVGGGRVAAHEIMLATPAIRNLIREDKIAQMYSSIQTGASQGMQTMDQCL
TNLVNRGIITHSAAYTKAQDKTQFSA

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=1090667 ACLKMI_RS10020 WP_010391590.1 2089325..2090365(-) (pilT) [Pseudoalteromonas sp. KJ71-7]
ATGGATATCACCGAATTATTGGCATTTAGTGTGCAACATAAAGCATCAGATTTGCACTTATCATCAGGGGTGTCACCTAT
GATACGTGTTGATGGCGATGTGCGACGAATTAATATACCAGCACTTGGAGATAAAGAAGTCAGCAGTCTGGTTTATGATA
TTATGAACGATAACCAGCGTAAGGACTATGAACAAAATCTCGAAGTGGATTTCTCTTTTGAAGTCCCTAATTTAGCACGT
TTTCGTGTAAATGCCTTTAACTCTAATCGTGGTCCTGCTGCCGTGTTCCGTACAATCCCGAGTGACGTACTGACACTTGA
AGATTTAGGCGCGCCGGAAATATTTAAACAAATTTCAGATAACCCACGAGGCTTAGTCTTAGTTACCGGTCCTACAGGCT
CAGGTAAGTCGACTACACTCGCAGCCATGGTGGACTATATAAATCAAAATAAACATCACCATGTACTGACCATTGAAGAT
CCTATCGAATTTGTGCACGATAATAAACTCAGCTTAATTAACCAGCGTGAAGTTCACCGCGATACACATAGTTTTTCTAA
TGCCTTGCGAAGTGCATTGCGTGAAGACCCTGATGTTATTTTGGTTGGTGAGTTACGAGATCTAGAAACTATTCGTCTTG
CTATGACTGCGGCTGAAACCGGTCACTTAGTATTTGGTACTTTGCATACCACGTCGGCACCTAAAACCATTGACCGTATT
ATTGACGTATTTCCTGGTGAAGAAAAAGACATGATCCGCTCTATGCTTTCCGAATCATTGCGTGCGGTTATCTCACAAAC
ACTGATCAAGAAGGTCGGTGGTGGGCGAGTGGCTGCACACGAAATTATGTTGGCAACGCCCGCTATTCGTAACTTAATTC
GTGAAGATAAAATTGCTCAAATGTATTCATCAATTCAAACCGGTGCATCGCAAGGGATGCAAACCATGGATCAATGTTTA
ACGAATCTAGTTAACCGAGGCATTATCACTCACTCTGCGGCGTATACAAAAGCACAAGATAAGACGCAGTTTAGCGCTTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A317FVK0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

76.163

99.422

0.757
  pilT Acinetobacter nosocomialis M2

76.163

99.422

0.757
  pilT Acinetobacter baumannii D1279779

76.163

99.422

0.757
  pilT Acinetobacter baumannii strain A118

76.163

99.422

0.757
  pilT Pseudomonas aeruginosa PAK

75.581

99.422

0.751
  pilT Acinetobacter baylyi ADP1

75.291

99.422

0.749
  pilT Legionella pneumophila strain ERS1305867

71.802

99.422

0.714
  pilT Legionella pneumophila strain Lp02

71.802

99.422

0.714
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

73.252

95.087

0.697
  pilT Vibrio cholerae strain A1552

73.252

95.087

0.697
  pilT Neisseria gonorrhoeae MS11

69.364

100

0.694
  pilT Neisseria meningitidis 8013

69.364

100

0.694
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.023

100

0.52
  pilU Vibrio cholerae strain A1552

42.154

93.931

0.396
  pilU Pseudomonas stutzeri DSM 10701

39.233

97.977

0.384
  pilU Acinetobacter baylyi ADP1

38.824

98.266

0.382

Multiple sequence alignment