Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACLKMI_RS10015 Genome accession   NZ_CP178625
Coordinates   2088179..2089315 (-) Length   378 a.a.
NCBI ID   WP_413400782.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. KJ71-7     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2083179..2094315
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLKMI_RS09985 (ACLKMI_09985) - 2083557..2084450 (+) 894 WP_010391581.1 hypothetical protein -
  ACLKMI_RS09990 (ACLKMI_09990) - 2084528..2085001 (+) 474 WP_010391582.1 hypothetical protein -
  ACLKMI_RS09995 (ACLKMI_09995) rsmE 2085072..2085806 (+) 735 WP_413400781.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACLKMI_RS10000 (ACLKMI_10000) gshB 2085873..2086823 (+) 951 WP_010391584.1 glutathione synthase -
  ACLKMI_RS10005 (ACLKMI_10005) - 2086951..2087508 (+) 558 WP_010391585.1 YqgE/AlgH family protein -
  ACLKMI_RS10010 (ACLKMI_10010) ruvX 2087526..2087975 (+) 450 WP_010391588.1 Holliday junction resolvase RuvX -
  ACLKMI_RS10015 (ACLKMI_10015) pilU 2088179..2089315 (-) 1137 WP_413400782.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACLKMI_RS10020 (ACLKMI_10020) pilT 2089325..2090365 (-) 1041 WP_010391590.1 type IV pilus twitching motility protein PilT Machinery gene
  ACLKMI_RS10025 (ACLKMI_10025) - 2090368..2091081 (+) 714 WP_413400783.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACLKMI_RS10030 (ACLKMI_10030) proC 2091099..2091920 (+) 822 WP_138609266.1 pyrroline-5-carboxylate reductase -
  ACLKMI_RS10035 (ACLKMI_10035) - 2091947..2092480 (+) 534 WP_008113334.1 YggT family protein -
  ACLKMI_RS10040 (ACLKMI_10040) - 2092539..2092982 (+) 444 WP_413400784.1 DUF4426 domain-containing protein -
  ACLKMI_RS10045 (ACLKMI_10045) - 2092985..2093584 (+) 600 WP_413400785.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41947.95 Da        Isoelectric Point: 6.2895

>NTDB_id=1090666 ACLKMI_RS10015 WP_413400782.1 2088179..2089315(-) (pilU) [Pseudoalteromonas sp. KJ71-7]
MTLSLNHFLQIMIDKKGSDLFVSSQLPISAKINGELTPLNDDKLTDEQALELVESAMSEKQKAEFHNTKECNFAIATSEG
RFRISAFWQRDRAGMVIRRIVTQIPDVKELGLPSALTDVIMSKRGLVLFVGGTGTGKSTSLAALIGYRNRNQRGHILTIE
DPIEFVHEHKKSIITQREVGLDTESFESALKSSLRQAPDVILIGEIRSQETMEYALSFAETGHLCVATLHANNANQAIDR
IMHLVPKEKHDKLKYDLALNLRAIIAQQLIPTSDGEGRAAAIEVLLNTPMVSELIKNGNIGGIKETMAKSTEMGMQTFDQ
ALFELYRQQRINYADALHHADSPNDLRLMIKLRNNEQQGAGFLQGVTIDGLDDKGNIT

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=1090666 ACLKMI_RS10015 WP_413400782.1 2088179..2089315(-) (pilU) [Pseudoalteromonas sp. KJ71-7]
ATGACTTTATCTCTAAATCACTTTTTACAAATAATGATCGACAAAAAAGGCTCTGATTTATTTGTATCAAGCCAGCTGCC
TATCAGCGCTAAAATTAATGGTGAGCTCACACCTCTTAATGATGACAAACTAACCGATGAGCAAGCGCTGGAATTAGTTG
AATCAGCGATGAGTGAAAAGCAAAAAGCAGAGTTTCATAACACTAAAGAGTGTAACTTTGCGATTGCAACCAGTGAAGGG
CGCTTTCGTATTTCGGCTTTTTGGCAACGCGACAGAGCGGGGATGGTTATTCGCCGGATTGTGACCCAAATACCTGATGT
AAAAGAACTTGGATTGCCCTCAGCGCTTACTGATGTGATTATGTCAAAGCGCGGACTGGTATTATTTGTCGGTGGTACGG
GAACAGGTAAATCAACCTCTTTGGCGGCGCTTATTGGTTATAGAAATCGTAATCAACGAGGGCATATTTTAACTATAGAA
GATCCCATTGAGTTTGTGCATGAACATAAAAAAAGCATTATTACTCAACGTGAAGTGGGCTTAGATACTGAAAGTTTTGA
GTCAGCACTCAAAAGCTCTTTACGACAAGCGCCTGATGTTATTTTAATTGGTGAAATTCGATCGCAAGAAACTATGGAGT
ATGCGTTAAGCTTTGCAGAAACAGGTCATCTATGTGTTGCCACCTTGCATGCTAACAATGCTAACCAAGCTATTGATCGT
ATTATGCATTTAGTGCCAAAAGAGAAGCACGATAAGCTTAAATATGATTTAGCATTAAACTTACGCGCTATTATTGCCCA
GCAGCTTATCCCAACCTCTGATGGGGAGGGACGTGCTGCTGCTATAGAGGTATTATTGAATACGCCAATGGTGAGTGAGC
TGATTAAAAACGGTAACATTGGTGGTATTAAAGAAACTATGGCCAAATCAACTGAAATGGGCATGCAGACCTTTGACCAA
GCGCTGTTTGAGCTTTACAGACAGCAACGGATTAACTATGCCGATGCACTTCATCATGCAGACTCGCCAAATGATTTACG
TTTAATGATAAAACTGCGTAATAACGAGCAGCAAGGGGCAGGCTTTTTGCAAGGTGTAACCATTGATGGCCTTGATGATA
AAGGTAATATTACCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

57.294

99.735

0.571
  pilU Pseudomonas stutzeri DSM 10701

57.333

99.206

0.569
  pilU Vibrio cholerae strain A1552

56.403

97.09

0.548
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.944

95.238

0.399

Multiple sequence alignment