Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACJQW4_RS02160 Genome accession   NZ_CP178606
Coordinates   468160..469278 (+) Length   372 a.a.
NCBI ID   WP_241626919.1    Uniprot ID   -
Organism   Acinetobacter sp. A7.4     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 463160..474278
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJQW4_RS02140 (ACJQW4_02140) - 463405..464430 (+) 1026 WP_241626925.1 class I SAM-dependent methyltransferase -
  ACJQW4_RS02145 (ACJQW4_02145) - 464440..465903 (+) 1464 WP_241626923.1 amino acid permease -
  ACJQW4_RS02150 (ACJQW4_02150) - 466283..466969 (-) 687 WP_241626921.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACJQW4_RS02155 (ACJQW4_02155) pilT 467093..468130 (+) 1038 WP_100242154.1 type IV pilus twitching motility protein PilT Machinery gene
  ACJQW4_RS02160 (ACJQW4_02160) pilU 468160..469278 (+) 1119 WP_241626919.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACJQW4_RS02165 (ACJQW4_02165) omp38 469419..470438 (-) 1020 WP_241626917.1 outer membrane protein Omp38 -
  ACJQW4_RS02170 (ACJQW4_02170) - 470815..471333 (+) 519 WP_241626916.1 GspH/FimT family pseudopilin -
  ACJQW4_RS02175 (ACJQW4_02175) - 471435..472610 (+) 1176 WP_241626914.1 thiolase family protein -
  ACJQW4_RS02180 (ACJQW4_02180) - 472757..473203 (+) 447 WP_241626912.1 VOC family protein -
  ACJQW4_RS02185 (ACJQW4_02185) - 473293..473778 (-) 486 WP_241626910.1 DUF934 domain-containing protein -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41921.27 Da        Isoelectric Point: 6.8367

>NTDB_id=1090384 ACJQW4_RS02160 WP_241626919.1 468160..469278(+) (pilU) [Acinetobacter sp. A7.4]
MDFNDLLNLMIQQKASDLFVTAEVEPSMKINGQIVPVAKTKLTGEIVGQLINSIMSDKQRKEFTETRECNFAIMNREKTA
RFRVSAFQQRDQPGMVLRRIETKIPTMDELKLPPVLKDLAMTKRGIIIFVGATGTGKSTSLASIIGYRNQNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVFATLHANNANQAIDR
IIHFFEADRHSQLFMDLSLNLKAMVAQQLIPTIDGNSRRAAIEILINSPLISDLIRKGDVHEIKDLMKRSRELGMQTFDQ
ALYDLYKAKQITYKDALKHADSPNDLRLQIKLSEEGADRLMNASSHITFDGQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=1090384 ACJQW4_RS02160 WP_241626919.1 468160..469278(+) (pilU) [Acinetobacter sp. A7.4]
ATGGACTTTAACGACTTACTCAATTTAATGATTCAACAAAAAGCTTCGGATCTTTTTGTCACGGCTGAAGTTGAACCATC
GATGAAAATTAATGGTCAAATTGTACCCGTTGCAAAGACCAAGCTGACTGGTGAGATTGTTGGTCAACTGATTAATTCAA
TCATGAGCGACAAACAGCGTAAAGAATTTACTGAGACTCGTGAATGTAACTTTGCGATTATGAATCGCGAAAAAACAGCG
CGTTTTCGTGTTAGTGCTTTTCAACAACGTGATCAACCCGGCATGGTTCTTCGTCGCATTGAAACCAAAATTCCAACCAT
GGATGAGCTTAAACTTCCTCCTGTATTGAAAGATTTAGCCATGACCAAACGTGGCATCATTATCTTTGTAGGTGCAACAG
GGACAGGTAAATCGACTTCTTTGGCATCGATCATTGGTTATCGCAACCAAAATTCCAAAGGTCATATTATTACCATTGAA
GACCCGATTGAATTTATTCACGAACATGCAGGCTGTATTATTACCCAACGTGAAGTGGGTATTGATACAGACTCTTTTGA
AATCGCATTAAAAAATACTTTACGTCAGGCACCGGATGTCATTCTGATTGGTGAGATTCGATCACGTGAGACTATGGACT
ATGCGATTGCCTTTGCCGAAACAGGACATTTAGTTTTCGCAACACTACATGCCAACAACGCTAACCAAGCCATTGACCGT
ATTATCCACTTCTTTGAAGCTGATCGTCATAGTCAATTATTTATGGACTTGTCTTTAAATCTAAAAGCGATGGTGGCTCA
ACAACTGATTCCAACTATTGATGGCAATTCACGTCGAGCTGCGATTGAAATTCTGATTAACTCCCCTTTAATTTCCGACT
TGATCCGTAAAGGTGATGTTCATGAAATTAAAGACCTTATGAAGCGTTCACGTGAACTCGGCATGCAGACCTTTGACCAA
GCACTCTACGATCTATATAAAGCCAAGCAAATCACCTATAAAGATGCGCTGAAACATGCTGATTCGCCAAACGACTTACG
TTTACAAATCAAACTTTCAGAAGAAGGGGCGGATCGTTTAATGAATGCCAGCTCACATATTACCTTTGATGGTCAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

76.455

100

0.777
  pilU Pseudomonas stutzeri DSM 10701

68.733

99.731

0.685
  pilU Vibrio cholerae strain A1552

55.556

94.355

0.524
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.758

97.849

0.409
  pilT Pseudomonas aeruginosa PAK

41.04

93.011

0.382
  pilT Acinetobacter baylyi ADP1

40.233

92.204

0.371
  pilT Acinetobacter nosocomialis M2

40.233

92.204

0.371
  pilT Acinetobacter baumannii D1279779

40.233

92.204

0.371
  pilT Acinetobacter baumannii strain A118

40.233

92.204

0.371
  pilT Pseudomonas stutzeri DSM 10701

39.884

93.011

0.371
  pilT Legionella pneumophila strain Lp02

41.071

90.323

0.371
  pilT Legionella pneumophila strain ERS1305867

41.071

90.323

0.371
  pilT Vibrio cholerae strain A1552

39.031

94.355

0.368
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.031

94.355

0.368
  pilT Neisseria gonorrhoeae MS11

39.412

91.398

0.36
  pilT Neisseria meningitidis 8013

39.412

91.398

0.36

Multiple sequence alignment