Detailed information NTG1090354

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: ACL656_RS03885
• Genome accession: NZ_CP178597
• Coordinates: 821309..822289 (+)
• Length: 326 a.a.
• NCBI ID: WP_072685927.1
• Uniprot ID: -
• Organism: Escherichia coli strain ZF8-1
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 816309..827289

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACL656_RS03855 (ACL656_03855)	yggM	816736..817743 (+)	1008	WP_129949048.1	DUF1202 family protein	-
ACL656_RS03860 (ACL656_03860)	hemW	817986..819122 (-)	1137	WP_001625906.1	radical SAM family heme chaperone HemW	-
ACL656_RS03865 (ACL656_03865)	rdgB	819115..819708 (-)	594	WP_001174750.1	XTP/dITP diphosphatase	-
ACL656_RS03870 (ACL656_03870)	yggU	819716..820006 (-)	291	WP_001277222.1	DUF167 family protein YggU	-
ACL656_RS03875 (ACL656_03875)	yggT	820003..820569 (-)	567	WP_001094831.1	osmotic shock tolerance protein YggT	-
ACL656_RS03880 (ACL656_03880)	yggS	820587..821291 (-)	705	WP_000997795.1	pyridoxal phosphate homeostasis protein	-
ACL656_RS03885 (ACL656_03885)	pilT	821309..822289 (+)	981	WP_072685927.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACL656_RS03890 (ACL656_03890)	ruvX	822480..822896 (-)	417	WP_072685925.1	Holliday junction resolvase RuvX	-
ACL656_RS03895 (ACL656_03895)	yqgE	822896..823459 (-)	564	WP_001053178.1	YqgE/AlgH family protein	-
ACL656_RS03900 (ACL656_03900)	gshB	823568..824518 (-)	951	WP_000593273.1	glutathione synthase	-
ACL656_RS03905 (ACL656_03905)	rsmE	824531..825262 (-)	732	WP_001222509.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
ACL656_RS03910 (ACL656_03910)	endA	825342..826049 (-)	708	WP_000286500.1	deoxyribonuclease I	-
ACL656_RS03915 (ACL656_03915)	yggI	826144..826641 (-)	498	WP_001300769.1	SprT family zinc-dependent metalloprotease	-

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 35935.14 Da
• Isoelectric Point: 5.5860

>NTDB_id=1090354 ACL656_RS03885 WP_072685927.1 821309..822289(+) (pilT) [Escherichia coli strain ZF8-1]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISMALRLLPSHCPQLEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VAEGRL

Nucleotide

• Length: 981 bp

>NTDB_id=1090354 ACL656_RS03885 WP_072685927.1 821309..822289(+) (pilT) [Escherichia coli strain ZF8-1]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCACAACGGCAAGGTATTTCTATGGCGTTACGGTTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCAACGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAGATTGGTTTGCACTGTATGACGTTTGCATCGGGATTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCAACCTTACATACACGTGGTGCCGCGCAGGCAGTTGAGCGACTTGTGGATTCA
TTTCCGGCGCAGGAAAAAGACCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTATTTGAATTGCTGATTAACACACCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGA
GTGGCGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (63-239)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae strain A1552	49.847	100	0.5
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	49.847	100	0.5
pilT	Neisseria meningitidis 8013	48.78	100	0.491
pilT	Neisseria gonorrhoeae MS11	48.476	100	0.488
pilT	Acinetobacter baumannii strain A118	46.789	100	0.469
pilT	Acinetobacter baylyi ADP1	46.789	100	0.469
pilT	Acinetobacter baumannii D1279779	46.789	100	0.469
pilT	Pseudomonas stutzeri DSM 10701	46.789	100	0.469
pilT	Acinetobacter nosocomialis M2	46.483	100	0.466
pilT	Pseudomonas aeruginosa PAK	46.177	100	0.463
pilT	Legionella pneumophila strain Lp02	45.912	97.546	0.448
pilT	Legionella pneumophila strain ERS1305867	45.912	97.546	0.448
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.138	97.546	0.411
pilU	Vibrio cholerae strain A1552	40.694	97.239	0.396
pilU	Pseudomonas stutzeri DSM 10701	37.879	100	0.383
pilU	Acinetobacter baylyi ADP1	37.037	99.387	0.368
pilB	Legionella pneumophila strain ERS1305867	31.152	100	0.365