Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACLWNE_RS10050 Genome accession   NZ_CP178485
Coordinates   1917760..1918851 (+) Length   363 a.a.
NCBI ID   WP_339956755.1    Uniprot ID   -
Organism   Thermus oshimai strain Blanco     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1912760..1923851
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLWNE_RS10025 (ACLWNE_10025) ribH 1912912..1913388 (+) 477 WP_041436569.1 6,7-dimethyl-8-ribityllumazine synthase -
  ACLWNE_RS10030 (ACLWNE_10030) - 1913372..1913776 (-) 405 WP_016330149.1 DUF4395 domain-containing protein -
  ACLWNE_RS10035 (ACLWNE_10035) pgeF 1913847..1914575 (+) 729 WP_413217186.1 peptidoglycan editing factor PgeF -
  ACLWNE_RS10040 (ACLWNE_10040) - 1914600..1915085 (+) 486 WP_018461203.1 YqeG family HAD IIIA-type phosphatase -
  ACLWNE_RS10045 (ACLWNE_10045) pilF 1915082..1917748 (+) 2667 WP_413217187.1 type IV pilus assembly ATPase PilB Machinery gene
  ACLWNE_RS10050 (ACLWNE_10050) pilT 1917760..1918851 (+) 1092 WP_339956755.1 type IV pilus twitching motility protein PilT Machinery gene
  ACLWNE_RS10055 (ACLWNE_10055) gatB 1918869..1920299 (+) 1431 WP_339956754.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB -
  ACLWNE_RS10060 (ACLWNE_10060) purM 1920304..1921305 (+) 1002 WP_339956753.1 phosphoribosylformylglycinamidine cyclo-ligase -
  ACLWNE_RS10065 (ACLWNE_10065) - 1921302..1921931 (+) 630 WP_018461198.1 histidine phosphatase family protein -

Sequence

Protein


Download         Length: 363 a.a.        Molecular weight: 40275.62 Da        Isoelectric Point: 7.2371

>NTDB_id=1089709 ACLWNE_RS10050 WP_339956755.1 1917760..1918851(+) (pilT) [Thermus oshimai strain Blanco]
MPKAPDIVDLLSLAVERGASDLVITVGLPPMIKVDGEFHPTEYEPLTPQETRRLMYALMDEKQQRVFEEEKELDFSFSLP
GKGRYRVNVFLQRGSVGGVLRVVPAQIKSFEELGLPKNIAEIAMSPRGLVLVTGPTGSGKSTTLASMIDYINERKPVHIV
TIEDPIEFFHRHKRAIINQREIGSDTHGFHKALRSVLRQAPDVILVGEMRDYETIAAAITAAETGHLVMGTLHTNSAPET
IDRIIDVFPENQQEQVRVQLSNNLVAVLTQQLLPKAFGGGRVLAYELMIATPAVRALIREGKSHQLRSVIQTGGQYGMVT
MDACLADLYKRKLITYEMGLARAVDPKEFMRLAGVGEKEGRPK

Nucleotide


Download         Length: 1092 bp        

>NTDB_id=1089709 ACLWNE_RS10050 WP_339956755.1 1917760..1918851(+) (pilT) [Thermus oshimai strain Blanco]
ATGCCGAAAGCGCCGGACATCGTAGATCTGCTTTCCCTGGCCGTGGAGCGGGGCGCCAGCGACCTGGTCATCACCGTGGG
CCTTCCGCCCATGATCAAGGTGGACGGGGAGTTCCACCCCACGGAGTACGAGCCCCTCACCCCCCAGGAGACCCGCCGCC
TCATGTACGCCCTCATGGACGAGAAGCAGCAGCGGGTCTTTGAGGAGGAGAAGGAGCTGGACTTCTCCTTCAGCCTCCCC
GGCAAGGGGCGCTACCGGGTCAACGTCTTCCTCCAGCGGGGGAGCGTGGGCGGGGTCTTGCGGGTGGTGCCGGCCCAGAT
CAAGAGCTTTGAGGAGCTGGGCCTGCCCAAGAACATCGCCGAGATCGCCATGAGCCCCCGGGGCCTGGTCCTGGTCACGG
GGCCTACGGGAAGCGGGAAGAGCACCACCCTGGCCTCCATGATCGACTACATCAACGAACGCAAGCCCGTGCACATCGTG
ACCATCGAGGACCCCATCGAGTTCTTCCACCGCCACAAGAGGGCCATCATCAACCAGCGGGAGATCGGTTCGGACACCCA
CGGCTTCCACAAGGCCCTAAGGAGCGTCCTCCGCCAGGCCCCGGACGTGATCCTGGTGGGGGAGATGCGGGACTACGAGA
CCATCGCCGCGGCCATCACCGCCGCAGAGACCGGCCACCTGGTCATGGGCACCCTGCACACCAACTCCGCCCCCGAGACC
ATCGACCGCATCATCGACGTCTTCCCCGAGAACCAACAGGAGCAGGTGCGGGTGCAGCTTTCCAACAACCTGGTGGCCGT
CCTCACCCAGCAGCTCCTGCCCAAGGCCTTCGGCGGGGGCAGGGTCCTGGCCTACGAGCTCATGATCGCCACCCCCGCGG
TGCGGGCCCTCATCCGCGAGGGGAAGAGCCACCAGCTAAGGAGCGTGATCCAGACCGGGGGGCAGTACGGGATGGTCACC
ATGGACGCCTGCCTGGCGGACCTCTACAAGCGCAAGCTCATCACCTACGAGATGGGCCTGGCCCGGGCCGTGGACCCCAA
GGAGTTCATGCGCCTGGCGGGGGTGGGGGAAAAGGAGGGCCGGCCCAAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

67.422

97.245

0.656
  pilT Acinetobacter baylyi ADP1

54.942

94.766

0.521
  pilT Acinetobacter nosocomialis M2

53.913

95.041

0.512
  pilT Acinetobacter baumannii strain A118

53.913

95.041

0.512
  pilT Acinetobacter baumannii D1279779

53.913

95.041

0.512
  pilT Pseudomonas aeruginosa PAK

53.779

94.766

0.51
  pilT Pseudomonas stutzeri DSM 10701

53.488

94.766

0.507
  pilT Neisseria meningitidis 8013

52.071

93.113

0.485
  pilT Legionella pneumophila strain ERS1305867

50.872

94.766

0.482
  pilT Legionella pneumophila strain Lp02

50.872

94.766

0.482
  pilT Vibrio cholerae strain A1552

52.584

90.634

0.477
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.584

90.634

0.477
  pilT Neisseria gonorrhoeae MS11

51.335

92.837

0.477
  pilU Pseudomonas stutzeri DSM 10701

46.939

94.49

0.444
  pilU Acinetobacter baylyi ADP1

41.899

98.623

0.413
  pilU Vibrio cholerae strain A1552

44.012

92.011

0.405

Multiple sequence alignment