Detailed information NTG1089708

Bioinformatically predicted

Overview

• Name: pilF
• Type: Machinery gene
• Locus tag: ACLWNE_RS10045
• Genome accession: NZ_CP178485
• Coordinates: 1915082..1917748 (+)
• Length: 888 a.a.
• NCBI ID: WP_413217187.1
• Uniprot ID: -
• Organism: Thermus oshimai strain Blanco
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1910082..1922748

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACLWNE_RS10000 (ACLWNE_10000)	-	1910468..1911271 (+)	804	WP_018461208.1	histidinol-phosphatase HisJ family protein	-
ACLWNE_RS10005 (ACLWNE_10005)	-	1911247..1911504 (-)	258	WP_018461207.1	hypothetical protein	-
ACLWNE_RS10010 (ACLWNE_10010)	-	1911529..1912134 (-)	606	WP_016330145.1	sulfite oxidase-like oxidoreductase	-
ACLWNE_RS10015 (ACLWNE_10015)	-	1912179..1912508 (-)	330	WP_016330146.1	DUF190 domain-containing protein	-
ACLWNE_RS10020 (ACLWNE_10020)	crcB	1912513..1912890 (-)	378	WP_018461206.1	fluoride efflux transporter CrcB	-
ACLWNE_RS10025 (ACLWNE_10025)	ribH	1912912..1913388 (+)	477	WP_041436569.1	6,7-dimethyl-8-ribityllumazine synthase	-
ACLWNE_RS10030 (ACLWNE_10030)	-	1913372..1913776 (-)	405	WP_016330149.1	DUF4395 domain-containing protein	-
ACLWNE_RS10035 (ACLWNE_10035)	pgeF	1913847..1914575 (+)	729	WP_413217186.1	peptidoglycan editing factor PgeF	-
ACLWNE_RS10040 (ACLWNE_10040)	-	1914600..1915085 (+)	486	WP_018461203.1	YqeG family HAD IIIA-type phosphatase	-
ACLWNE_RS10045 (ACLWNE_10045)	pilF	1915082..1917748 (+)	2667	WP_413217187.1	type IV pilus assembly ATPase PilB	Machinery gene
ACLWNE_RS10050 (ACLWNE_10050)	pilT	1917760..1918851 (+)	1092	WP_339956755.1	type IV pilus twitching motility protein PilT	Machinery gene
ACLWNE_RS10055 (ACLWNE_10055)	gatB	1918869..1920299 (+)	1431	WP_339956754.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB	-
ACLWNE_RS10060 (ACLWNE_10060)	purM	1920304..1921305 (+)	1002	WP_339956753.1	phosphoribosylformylglycinamidine cyclo-ligase	-
ACLWNE_RS10065 (ACLWNE_10065)	-	1921302..1921931 (+)	630	WP_018461198.1	histidine phosphatase family protein	-

Sequence

Protein

• Length: 888 a.a.
• Molecular weight: 97927.15 Da
• Isoelectric Point: 5.0923

>NTDB_id=1089708 ACLWNE_RS10045 WP_413217187.1 1915082..1917748(+) (pilF) [Thermus oshimai strain Blanco]
MSVLTIGDKRLGAILLDAGLLTDEELQMALERHREVGGSLAEVLVDMGLLSERRIAQAIEDHFGIPLVELHTLEIPPKVR
ALLPAEKAKELQAIPFALDEEAGVVRVAFVNPLDTLALEEVEDLTGLVVEPYQTTKSAFLYTLAKAYPELGLPVPPPPSG
PSGAEMRLGELLVEKGLISRDTLEEALVEQEKTGDLLGRILVRKGLDEKALYRVLAEQNGLEFLEDTAGLTPALEATRLL
LRSDALRYSAVPVGLKDGKVEVVLSDPRHKAQVEELLGKPAHFYLTLPKAWEELFHRVYPEKGRLGEVLVQEGKLSREAL
KEALAVQETLPKAKPLGEILVELGLVRPEDVEEALKKQRQGGGRLEDTLIQSGKLKPEALAQAMAAQLGYPYINPEESPP
DPGVALLLPEDLARRYGVFAHHLEGNTLVLLMREPRNIIVLDDLKQYFKRKGLNYTLAPAVAPEAAITKLIERFYGKAEL
GEIAKELSRGFKEEEAPSLDLDESAAQRFVKQVIREAYLQDASDIHIEPRQGDVLVRLRVDGTLRQYTTLPKGALGPVIS
VIKILGGLDIAERRLPQDGRVRYREGEVDLDLRLSTLPTVYGEKAVMRLLKKASDIPEIEGLGFAPEVFQRFKEVIEKPY
GIFLITGPTGSGKSFTTFSILKRIATPDKNTQTIEDPVEYEIPGINQTQVNPQAGLTFARALRAFLRQDPDIIMVGEIRD
SETAKIATEAALTGHLVIATLHTNDAAQAITRLDEMGVELFNISAALIGVLSQRLVRKICEGCKQEVKPDPEVLRRLGLS
EEEIRGAKLYKGLGCERCGGTGYKGRYAIHELLVVDDEIRHAIVAGKSATEIKEIARRKGMKTLREDGIYKALQGITTLE
EVLARTIE

Nucleotide

• Length: 2667 bp

>NTDB_id=1089708 ACLWNE_RS10045 WP_413217187.1 1915082..1917748(+) (pilF) [Thermus oshimai strain Blanco]
ATGAGCGTCCTGACCATCGGCGACAAGCGGCTTGGGGCCATCCTTTTGGACGCGGGCCTCCTCACGGACGAGGAGCTCCA
GATGGCCTTAGAGCGGCACCGGGAGGTGGGGGGGTCCTTGGCCGAGGTCCTGGTGGACATGGGCCTCCTCTCCGAGCGCC
GCATCGCCCAGGCCATAGAGGACCACTTCGGCATCCCCCTGGTGGAGCTCCACACCCTGGAGATCCCCCCCAAGGTGCGG
GCCCTTCTCCCCGCGGAGAAGGCCAAGGAGCTCCAGGCCATCCCCTTCGCCCTGGACGAGGAGGCGGGGGTGGTGCGGGT
GGCCTTCGTGAACCCCCTGGACACCCTCGCCCTGGAAGAGGTGGAGGACCTCACCGGGCTGGTGGTGGAACCCTACCAGA
CCACCAAGAGCGCCTTCCTCTACACCCTGGCCAAGGCCTACCCCGAGCTGGGCCTCCCCGTGCCCCCACCCCCTTCCGGG
CCCAGCGGGGCGGAGATGCGCCTGGGGGAGCTTCTGGTAGAGAAGGGCCTCATAAGCCGGGACACCCTGGAGGAGGCCCT
GGTGGAGCAGGAAAAGACGGGGGACCTCCTGGGGCGGATCCTGGTGCGAAAGGGGCTGGACGAGAAGGCCCTTTACCGGG
TCCTGGCGGAGCAGAACGGCCTGGAGTTCCTGGAGGACACCGCAGGGCTCACCCCCGCCCTCGAGGCCACCCGCCTCCTC
CTCCGCTCGGATGCCCTCCGCTACAGCGCGGTGCCCGTGGGCCTCAAGGACGGGAAGGTGGAGGTGGTCCTCTCTGACCC
CCGGCACAAGGCCCAGGTGGAGGAGCTTTTGGGCAAGCCGGCCCATTTCTACCTCACCCTGCCCAAGGCCTGGGAAGAGC
TCTTCCACCGGGTCTACCCGGAGAAGGGGCGGCTCGGGGAGGTGTTGGTCCAGGAGGGGAAGCTCTCCCGGGAGGCCCTG
AAGGAGGCCCTGGCGGTGCAGGAAACCCTCCCCAAGGCCAAGCCCCTGGGGGAGATCCTGGTGGAGCTGGGCCTCGTCCG
CCCCGAGGACGTGGAGGAGGCCCTGAAGAAGCAGCGCCAGGGTGGGGGGCGCCTGGAGGACACCCTGATCCAGTCCGGCA
AGCTCAAGCCCGAGGCCCTGGCCCAGGCCATGGCCGCCCAGCTGGGCTACCCCTACATCAACCCCGAGGAAAGCCCCCCC
GACCCCGGGGTGGCCCTCCTCCTCCCCGAGGACCTGGCCCGGCGCTACGGGGTCTTCGCCCACCACCTGGAGGGGAACAC
CCTGGTCCTCCTCATGCGGGAACCCCGGAACATCATCGTTCTGGACGACCTCAAGCAGTACTTCAAGCGCAAGGGGCTGA
ACTACACCCTGGCCCCCGCGGTGGCCCCGGAGGCGGCCATCACCAAGCTCATCGAGCGCTTCTACGGCAAGGCGGAACTG
GGGGAGATCGCCAAGGAGCTCTCCCGGGGCTTCAAGGAGGAGGAGGCCCCCAGCCTGGACCTGGACGAGAGCGCCGCCCA
GCGCTTCGTCAAGCAGGTGATCCGGGAGGCCTACCTCCAAGACGCCTCGGACATCCACATCGAACCCCGCCAGGGCGACG
TCCTGGTGCGCCTCCGGGTTGACGGCACCCTGCGCCAGTACACCACCCTGCCCAAAGGGGCCTTGGGACCGGTGATCAGC
GTGATCAAGATCCTGGGGGGTCTGGACATCGCGGAGAGGCGTCTCCCCCAGGACGGCCGCGTGCGCTACCGGGAAGGGGA
GGTGGACCTGGACCTCCGCCTCTCCACCCTGCCCACGGTCTACGGGGAGAAGGCCGTCATGCGCCTTTTGAAAAAGGCCT
CGGACATCCCCGAGATTGAGGGGCTTGGGTTCGCCCCCGAGGTCTTCCAGCGCTTTAAGGAGGTCATCGAAAAACCCTAC
GGCATCTTCCTCATCACCGGGCCCACAGGGTCAGGGAAGAGCTTCACCACCTTCTCCATCCTGAAGCGCATCGCCACCCC
CGACAAGAACACCCAGACCATCGAGGACCCCGTGGAGTACGAGATCCCGGGCATCAACCAGACCCAGGTGAACCCGCAGG
CCGGGCTCACCTTCGCCCGCGCGCTTAGGGCCTTCCTCCGGCAGGACCCGGACATCATCATGGTGGGGGAGATCCGGGAC
TCGGAAACGGCCAAGATCGCCACCGAGGCCGCCCTCACCGGCCACCTGGTCATCGCCACCCTGCACACCAACGATGCCGC
CCAGGCCATCACCCGCCTGGACGAGATGGGGGTGGAGCTCTTCAACATCTCCGCGGCCCTCATCGGGGTGCTCTCCCAGA
GGCTGGTGCGGAAGATCTGCGAGGGGTGCAAGCAGGAGGTGAAGCCCGACCCCGAGGTCCTAAGGCGCCTGGGGCTTAGC
GAAGAGGAGATCCGGGGGGCCAAGCTCTACAAGGGCCTGGGGTGCGAGCGGTGCGGGGGCACGGGGTACAAGGGCCGCTA
CGCCATCCACGAGCTTTTGGTGGTGGACGACGAGATCCGCCACGCCATCGTGGCGGGGAAGTCGGCCACGGAGATCAAGG
AGATCGCCCGCAGGAAGGGCATGAAGACCCTGCGGGAAGACGGGATCTACAAGGCCCTCCAGGGGATCACCACCCTCGAG
GAGGTCCTGGCCCGTACCATTGAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (512-778)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (369-479)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (172-303)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (44-148)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilF	Thermus thermophilus HB27	85.377	100	0.855
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	56.614	100	0.569