Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACL0K2_RS15500 Genome accession   NZ_CP178378
Coordinates   3364674..3365708 (+) Length   344 a.a.
NCBI ID   WP_019339777.1    Uniprot ID   A0A0D9AFI5
Organism   Pseudomonas sp. YJ42     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3359674..3370708
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACL0K2_RS15480 (ACL0K2_15480) - 3360108..3362069 (-) 1962 WP_239611241.1 dynamin-like GTPase family protein -
  ACL0K2_RS15485 (ACL0K2_15485) - 3362187..3362777 (-) 591 WP_045164005.1 YggT family protein -
  ACL0K2_RS15490 (ACL0K2_15490) proC 3363054..3363881 (-) 828 WP_413042971.1 pyrroline-5-carboxylate reductase -
  ACL0K2_RS15495 (ACL0K2_15495) - 3363894..3364607 (-) 714 WP_413042972.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACL0K2_RS15500 (ACL0K2_15500) pilT 3364674..3365708 (+) 1035 WP_019339777.1 type IV pilus twitching motility protein PilT Machinery gene
  ACL0K2_RS15505 (ACL0K2_15505) pilU 3365787..3366932 (+) 1146 WP_045164002.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACL0K2_RS15510 (ACL0K2_15510) - 3366933..3367763 (-) 831 WP_045164001.1 TIGR03915 family putative DNA repair protein -
  ACL0K2_RS15515 (ACL0K2_15515) - 3367763..3368974 (-) 1212 WP_413042973.1 putative DNA modification/repair radical SAM protein -
  ACL0K2_RS15520 (ACL0K2_15520) - 3369111..3369509 (+) 399 WP_045163999.1 NINE protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37930.67 Da        Isoelectric Point: 6.8360

>NTDB_id=1089244 ACL0K2_RS15500 WP_019339777.1 3364674..3365708(+) (pilT) [Pseudomonas sp. YJ42]
MDITELLAFSAKQGASDLHLSSGLPPMIRVDGDVRRINLPAMDHKQVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGEVFRKITDVPRGLVLVTGPTGSGKSTTLAAMLDYLNNTKYHHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQAVISQTLLKKVGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSSIQTGGSLGMQTLDSCL
KGLLAKGLISRDAAKEKAKQPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1089244 ACL0K2_RS15500 WP_019339777.1 3364674..3365708(+) (pilT) [Pseudomonas sp. YJ42]
ATGGATATCACAGAACTGCTGGCCTTCAGTGCCAAGCAGGGTGCGTCTGACTTGCACCTGTCTTCCGGCTTGCCGCCGAT
GATCCGCGTTGACGGTGACGTGCGCCGGATCAATCTGCCGGCGATGGACCACAAGCAGGTGCACGCACTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGATTTCGAGGAGTTCCTCGAGACTGACTTTTCGTTCGAAGTGCCCGGCGTGGCGCGC
TTCCGGGTCAACGCATTCAACCAGAATCGCGGCGCCGGTGCGGTGTTCCGGACCATTCCGTCGAAGGTGCTGACCATGGA
AGACCTCGGCATGGGCGAGGTCTTCCGCAAGATCACCGATGTGCCGCGAGGGCTGGTGCTGGTCACCGGGCCGACCGGTT
CGGGCAAGTCGACCACCCTGGCGGCGATGCTCGACTATCTGAACAACACCAAATATCACCACATCCTCACCATCGAGGAC
CCGATCGAGTTCGTCCACGAGTCGAAGAAGTGCCTGGTCAACCAGCGTGAAGTGCACCGCGACACATTGGGCTTCTCCGA
AGCCCTGCGCTCGGCGCTGCGTGAAGACCCGGACATCATCCTCGTGGGCGAGATGCGCGATCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGTCACCTGGTATTCGGCACCCTGCACACCACCTCGGCGGCCAAGACCATTGACCGCGTG
GTCGATGTGTTCCCGGCGGAAGAAAAATCCATGGTTCGCTCGATGCTCTCCGAATCGCTGCAGGCGGTGATCTCCCAGAC
CCTGCTGAAGAAGGTCGGTGGCGGTCGAGTGGCCGCGCACGAAATCATGATCGGTACACCGGCGATCCGAAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCCTCGATCCAGACCGGCGGTTCGCTGGGCATGCAGACGCTCGATTCCTGTCTC
AAGGGGCTGCTGGCCAAGGGGTTGATTTCGCGCGACGCAGCCAAAGAAAAGGCCAAACAGCCGGAAAATTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D9AFI5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

99.709

100

0.997
  pilT Pseudomonas aeruginosa PAK

94.186

100

0.942
  pilT Acinetobacter baumannii D1279779

84.012

100

0.84
  pilT Acinetobacter baumannii strain A118

84.012

100

0.84
  pilT Acinetobacter nosocomialis M2

83.721

100

0.837
  pilT Acinetobacter baylyi ADP1

83.43

100

0.834
  pilT Legionella pneumophila strain Lp02

74.26

98.256

0.73
  pilT Legionella pneumophila strain ERS1305867

74.26

98.256

0.73
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.843

97.965

0.674
  pilT Vibrio cholerae strain A1552

68.843

97.965

0.674
  pilT Neisseria gonorrhoeae MS11

67.246

100

0.674
  pilT Neisseria meningitidis 8013

67.246

100

0.674
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.464

100

0.526
  pilU Vibrio cholerae strain A1552

41.003

98.547

0.404
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.384

0.398
  pilU Acinetobacter baylyi ADP1

39.017

100

0.392

Multiple sequence alignment