Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACL0K2_RS15505 Genome accession   NZ_CP178378
Coordinates   3365787..3366932 (+) Length   381 a.a.
NCBI ID   WP_045164002.1    Uniprot ID   A0A0D9AEN8
Organism   Pseudomonas sp. YJ42     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3360787..3371932
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACL0K2_RS15485 (ACL0K2_15485) - 3362187..3362777 (-) 591 WP_045164005.1 YggT family protein -
  ACL0K2_RS15490 (ACL0K2_15490) proC 3363054..3363881 (-) 828 WP_413042971.1 pyrroline-5-carboxylate reductase -
  ACL0K2_RS15495 (ACL0K2_15495) - 3363894..3364607 (-) 714 WP_413042972.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACL0K2_RS15500 (ACL0K2_15500) pilT 3364674..3365708 (+) 1035 WP_019339777.1 type IV pilus twitching motility protein PilT Machinery gene
  ACL0K2_RS15505 (ACL0K2_15505) pilU 3365787..3366932 (+) 1146 WP_045164002.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACL0K2_RS15510 (ACL0K2_15510) - 3366933..3367763 (-) 831 WP_045164001.1 TIGR03915 family putative DNA repair protein -
  ACL0K2_RS15515 (ACL0K2_15515) - 3367763..3368974 (-) 1212 WP_413042973.1 putative DNA modification/repair radical SAM protein -
  ACL0K2_RS15520 (ACL0K2_15520) - 3369111..3369509 (+) 399 WP_045163999.1 NINE protein -
  ACL0K2_RS15525 (ACL0K2_15525) - 3369564..3370835 (-) 1272 WP_413042974.1 dihydroorotase -
  ACL0K2_RS15530 (ACL0K2_15530) - 3370835..3371851 (-) 1017 WP_045163997.1 aspartate carbamoyltransferase catalytic subunit -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 42430.68 Da        Isoelectric Point: 6.5054

>NTDB_id=1089245 ACL0K2_RS15505 WP_045164002.1 3365787..3366932(+) (pilU) [Pseudomonas sp. YJ42]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKILPVNKTPMSPEMTRETVHAVMNEQQRREFAENHECNFAISARGIGR
FRVSAFYQRNLAGMVLRRIETNIPTLEDLKLPDVLKKLAMTKRGLVLFVGATGTGKSTSLAAMIGYRNKNSSGHIISIED
PIEFIHQHQSCIVTQREVGIDTDSFDVALKNTLRQAPDVILIGEVRTRETMDYAVAFAETGHLCLATLHANNANQALDRI
INFFPADRQQQVWMDLSLNLKAIVAQQLIPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKSLMKRSTDLGMQTFDQA
LYNLYVQGEITYEDALLHADSSNDLRLMIKLGSETDGEHLTSVSQGLTLEVSDDDPGRSFR

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=1089245 ACL0K2_RS15505 WP_045164002.1 3365787..3366932(+) (pilU) [Pseudomonas sp. YJ42]
ATGGAATTCGAGAAACTGTTGCGCCTGATGGTCGAAAAGGGCGGATCCGATCTGTTCATTACCGCTGGTGTGCCGCCTTC
GATGAAGGTCAACGGCAAGATCCTGCCGGTGAACAAGACGCCCATGTCGCCGGAAATGACCCGCGAAACCGTGCATGCCG
TGATGAACGAGCAGCAGCGCCGCGAGTTCGCCGAGAACCACGAGTGCAACTTTGCCATCAGCGCGCGCGGCATCGGTCGC
TTCCGGGTCAGCGCCTTCTATCAGCGCAACCTCGCCGGGATGGTGCTGCGCCGTATCGAGACCAATATCCCGACGCTCGA
AGACCTGAAACTGCCGGACGTGCTGAAAAAGCTGGCGATGACCAAGCGTGGGCTGGTGCTGTTCGTCGGCGCTACCGGCA
CCGGCAAGTCCACTTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCAGCGGTCATATCATTTCCATCGAAGAC
CCCATCGAGTTCATTCACCAGCATCAGAGCTGCATCGTTACTCAGCGCGAGGTCGGTATCGATACCGATTCCTTCGACGT
GGCGCTGAAGAACACGCTGCGCCAAGCGCCCGATGTGATCCTCATCGGTGAGGTGCGGACCCGCGAGACCATGGACTATG
CGGTCGCGTTCGCCGAAACCGGCCACCTGTGCCTGGCCACGCTACACGCCAACAACGCCAACCAGGCACTGGATCGAATC
ATCAACTTCTTCCCCGCCGACCGGCAGCAGCAGGTCTGGATGGATCTCTCGCTCAATCTCAAGGCCATCGTCGCCCAGCA
ACTGATCCCCACGCCCGACGGTAAAGGCCGGCGGGCGGTCATTGAGGTATTGATCAATACGCCGCTGGCGGCTGACCTGA
TCCGCAAGGGTGAAGTGCACGAACTCAAATCACTGATGAAGCGCTCCACCGACCTCGGCATGCAGACGTTCGATCAGGCG
CTCTACAACCTTTACGTGCAGGGCGAGATCACCTACGAAGATGCGCTGCTGCATGCCGACTCGTCCAACGACCTGCGCCT
GATGATCAAATTGGGCTCGGAGACCGATGGCGAACATCTGACCTCCGTATCCCAAGGCCTCACTCTGGAAGTCAGCGACG
ACGACCCGGGTCGCAGCTTCCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D9AEN8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

97.113

100

0.971
  pilU Acinetobacter baylyi ADP1

66.106

93.701

0.619
  pilU Vibrio cholerae strain A1552

59.143

91.864

0.543
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.953

88.976

0.391
  pilT Neisseria meningitidis 8013

40.87

90.551

0.37
  pilT Legionella pneumophila strain Lp02

42.09

87.927

0.37
  pilT Legionella pneumophila strain ERS1305867

42.09

87.927

0.37
  pilT Neisseria gonorrhoeae MS11

40.87

90.551

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.42

88.714

0.367
  pilT Vibrio cholerae strain A1552

41.42

88.714

0.367
  pilT Pseudomonas aeruginosa PAK

41.493

87.927

0.365
  pilT Pseudomonas stutzeri DSM 10701

41.194

87.927

0.362

Multiple sequence alignment