Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACLF6L_RS14125 Genome accession   NZ_CP178333
Coordinates   3079206..3080240 (-) Length   344 a.a.
NCBI ID   WP_238616363.1    Uniprot ID   -
Organism   Ectothiorhodospira shaposhnikovii strain PHS-Q     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3074206..3085240
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLF6L_RS14105 (ACLF6L_14105) - 3074561..3075853 (+) 1293 WP_238616360.1 dihydroorotase -
  ACLF6L_RS14110 (ACLF6L_14110) - 3075879..3076754 (+) 876 WP_412852794.1 dihydroorotate dehydrogenase electron transfer subunit -
  ACLF6L_RS14115 (ACLF6L_14115) - 3076779..3077975 (-) 1197 WP_238616361.1 PilT/PilU family type 4a pilus ATPase -
  ACLF6L_RS14120 (ACLF6L_14120) pilU 3077993..3079159 (-) 1167 WP_238616362.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACLF6L_RS14125 (ACLF6L_14125) pilT 3079206..3080240 (-) 1035 WP_238616363.1 type IV pilus twitching motility protein PilT Machinery gene
  ACLF6L_RS14130 (ACLF6L_14130) - 3080371..3081057 (+) 687 WP_238616364.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACLF6L_RS14135 (ACLF6L_14135) proC 3081094..3081921 (+) 828 WP_238616365.1 pyrroline-5-carboxylate reductase -
  ACLF6L_RS14140 (ACLF6L_14140) - 3081931..3082482 (+) 552 WP_238616366.1 YggT family protein -
  ACLF6L_RS14145 (ACLF6L_14145) - 3082472..3082795 (+) 324 WP_008931390.1 DUF167 domain-containing protein -
  ACLF6L_RS14150 (ACLF6L_14150) - 3082785..3083462 (-) 678 WP_238616367.1 sulfite exporter TauE/SafE family protein -
  ACLF6L_RS14155 (ACLF6L_14155) yrfG 3083469..3084155 (-) 687 WP_238616368.1 GMP/IMP nucleotidase -
  ACLF6L_RS14160 (ACLF6L_14160) nudE 3084328..3084870 (+) 543 WP_008931393.1 ADP compounds hydrolase NudE -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38215.94 Da        Isoelectric Point: 7.0677

>NTDB_id=1088901 ACLF6L_RS14125 WP_238616363.1 3079206..3080240(-) (pilT) [Ectothiorhodospira shaposhnikovii strain PHS-Q]
MDITQLLAFSVKNGASDLHLSAGLPPMIRVDGDVRRINVPAMEHKDVHGMVYDIMNDKQRKDYEERMETDFSFEIPGLAR
FRVNAFNHNRGAGAVFRTIPTKILTLEELGCPKIFEKISEYPRGIVLVTGPTGSGKSTTLAAMINHKNETEYGHILTVED
PIEFVHESKKCLVNQREVHRDTLGFNEALRSALREDPDTILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAAEKDMVRAMLSESLRAVISQTLMKKIGGGRCAAHEIMLGTPAVRNLIRENKIAQMYSAIQTSQGVGMQTLDQSL
KELLQRGLISKEEARRKAANPDNL

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1088901 ACLF6L_RS14125 WP_238616363.1 3079206..3080240(-) (pilT) [Ectothiorhodospira shaposhnikovii strain PHS-Q]
ATGGATATCACCCAATTGCTGGCGTTCAGCGTCAAGAACGGTGCCTCCGACCTGCACCTGTCCGCGGGCCTGCCGCCCAT
GATCCGCGTGGACGGCGATGTTCGCCGCATCAATGTGCCCGCCATGGAGCACAAGGACGTCCACGGCATGGTGTACGACA
TCATGAACGACAAGCAGCGCAAGGACTACGAGGAGCGGATGGAAACCGACTTCTCCTTCGAGATTCCGGGGCTGGCCCGG
TTCCGTGTCAATGCCTTCAATCACAACCGGGGCGCCGGCGCCGTCTTCCGTACCATTCCCACCAAGATCCTCACCCTGGA
AGAGTTGGGCTGTCCCAAGATCTTCGAGAAGATTTCCGAATATCCCCGCGGCATCGTGCTGGTGACCGGCCCCACCGGTT
CGGGCAAGTCCACCACCCTGGCGGCCATGATCAATCACAAGAACGAGACCGAGTACGGCCACATCCTGACGGTGGAAGAT
CCCATCGAGTTCGTCCACGAGTCCAAGAAATGCCTGGTGAACCAGCGCGAAGTGCACCGGGATACCCTGGGTTTCAACGA
GGCCCTGCGCTCCGCCTTGCGCGAGGACCCGGACACCATCCTGGTGGGGGAAATGCGGGATCTGGAAACCATCCGTCTGG
CCCTGACGGCAGCGGAGACCGGTCACCTGGTCTTCGGTACCCTGCACACCAGTTCCGCCGCCAAGACCATCGACCGTATC
GTCGACGTCTTCCCCGCGGCGGAAAAGGACATGGTGCGGGCGATGCTGTCCGAATCCCTGCGGGCGGTCATTTCCCAGAC
CCTGATGAAGAAGATCGGCGGCGGTCGCTGCGCCGCCCACGAGATCATGTTGGGTACCCCGGCGGTGCGAAACCTCATCC
GCGAGAACAAGATCGCGCAGATGTATTCCGCCATCCAGACCAGCCAGGGCGTGGGCATGCAGACCCTGGATCAGAGTCTC
AAGGAGCTCCTGCAGCGGGGCCTGATCAGCAAGGAAGAGGCCCGCCGCAAGGCCGCCAACCCGGACAACCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

80.523

100

0.805
  pilT Acinetobacter baumannii D1279779

80.523

100

0.805
  pilT Acinetobacter baumannii strain A118

80.523

100

0.805
  pilT Pseudomonas aeruginosa PAK

79.942

100

0.799
  pilT Pseudomonas stutzeri DSM 10701

79.36

100

0.794
  pilT Acinetobacter baylyi ADP1

78.488

100

0.785
  pilT Legionella pneumophila strain Lp02

72.941

98.837

0.721
  pilT Legionella pneumophila strain ERS1305867

72.941

98.837

0.721
  pilT Neisseria meningitidis 8013

69.275

100

0.695
  pilT Neisseria gonorrhoeae MS11

68.986

100

0.692
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.139

97.965

0.677
  pilT Vibrio cholerae strain A1552

69.139

97.965

0.677
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.17

99.419

0.509
  pilU Pseudomonas stutzeri DSM 10701

41.261

100

0.419
  pilU Vibrio cholerae strain A1552

41.159

100

0.413
  pilU Acinetobacter baylyi ADP1

39.884

100

0.401

Multiple sequence alignment