Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACLF6L_RS14120 Genome accession   NZ_CP178333
Coordinates   3077993..3079159 (-) Length   388 a.a.
NCBI ID   WP_238616362.1    Uniprot ID   -
Organism   Ectothiorhodospira shaposhnikovii strain PHS-Q     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3072993..3084159
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLF6L_RS14095 (ACLF6L_14095) pyrR 3073036..3073545 (+) 510 WP_238616359.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  ACLF6L_RS14100 (ACLF6L_14100) - 3073542..3074564 (+) 1023 WP_008931381.1 aspartate carbamoyltransferase catalytic subunit -
  ACLF6L_RS14105 (ACLF6L_14105) - 3074561..3075853 (+) 1293 WP_238616360.1 dihydroorotase -
  ACLF6L_RS14110 (ACLF6L_14110) - 3075879..3076754 (+) 876 WP_412852794.1 dihydroorotate dehydrogenase electron transfer subunit -
  ACLF6L_RS14115 (ACLF6L_14115) - 3076779..3077975 (-) 1197 WP_238616361.1 PilT/PilU family type 4a pilus ATPase -
  ACLF6L_RS14120 (ACLF6L_14120) pilU 3077993..3079159 (-) 1167 WP_238616362.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACLF6L_RS14125 (ACLF6L_14125) pilT 3079206..3080240 (-) 1035 WP_238616363.1 type IV pilus twitching motility protein PilT Machinery gene
  ACLF6L_RS14130 (ACLF6L_14130) - 3080371..3081057 (+) 687 WP_238616364.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACLF6L_RS14135 (ACLF6L_14135) proC 3081094..3081921 (+) 828 WP_238616365.1 pyrroline-5-carboxylate reductase -
  ACLF6L_RS14140 (ACLF6L_14140) - 3081931..3082482 (+) 552 WP_238616366.1 YggT family protein -
  ACLF6L_RS14145 (ACLF6L_14145) - 3082472..3082795 (+) 324 WP_008931390.1 DUF167 domain-containing protein -
  ACLF6L_RS14150 (ACLF6L_14150) - 3082785..3083462 (-) 678 WP_238616367.1 sulfite exporter TauE/SafE family protein -
  ACLF6L_RS14155 (ACLF6L_14155) yrfG 3083469..3084155 (-) 687 WP_238616368.1 GMP/IMP nucleotidase -

Sequence

Protein


Download         Length: 388 a.a.        Molecular weight: 43236.69 Da        Isoelectric Point: 6.3155

>NTDB_id=1088900 ACLF6L_RS14120 WP_238616362.1 3077993..3079159(-) (pilU) [Ectothiorhodospira shaposhnikovii strain PHS-Q]
MDRDKAIRFMHDLLKTLLSKKGSDLFITVGAPPSMKIDGKMTPITNQPLSPAHTQVLVRSIMNDRQAAEFERTQECNFAI
SLPGISRFRVNAFTQRGSSGMVLRVITSEIPSLDDLGLPMQLKDISMTRRGLVIFVGGTGSGKSTSLAAMVGYRNQNSYG
HIVTIEDPIEFVHSHVNCLVTQREVGVDTENYEIALKNTLRQAPDVILIGEIRDRETMDYAIAFAETGHLCLSTLHANST
NQALDRIINFFPEERRQQLLMDLSLNLKAVVSQRLIPLATGKGRVPAVEIMINSPLMSDLIFKGEIHEMKALMAKSNEAG
MQTFDQALFQLFETGRITYEDALRNADSVNDLRLRIKLDSKRARSQNLLDDTEGLEMEATPEESGLLK

Nucleotide


Download         Length: 1167 bp        

>NTDB_id=1088900 ACLF6L_RS14120 WP_238616362.1 3077993..3079159(-) (pilU) [Ectothiorhodospira shaposhnikovii strain PHS-Q]
ATGGATCGCGACAAGGCCATACGCTTCATGCACGACTTGCTCAAGACCCTCCTGAGCAAGAAGGGCTCCGACCTGTTCAT
TACCGTGGGTGCGCCGCCGTCCATGAAGATCGACGGCAAGATGACCCCCATCACCAATCAGCCGCTGTCGCCGGCCCATA
CCCAGGTGCTGGTGCGTTCCATCATGAATGACCGCCAGGCGGCGGAATTCGAACGGACCCAGGAGTGCAACTTCGCCATC
AGTCTGCCCGGGATTTCCCGCTTTCGCGTGAATGCCTTCACCCAGCGGGGTTCTTCCGGCATGGTGCTGCGGGTGATCAC
CTCGGAGATCCCGTCTCTGGACGATCTGGGTCTGCCGATGCAGCTCAAGGACATCTCCATGACCCGGCGCGGTCTGGTCA
TTTTCGTGGGGGGGACCGGTTCCGGCAAGTCCACCTCCCTGGCCGCCATGGTGGGTTATCGCAATCAGAACAGTTACGGG
CACATCGTCACCATCGAGGATCCCATCGAGTTCGTTCACTCCCACGTCAACTGCCTGGTGACCCAGCGGGAGGTGGGGGT
GGATACGGAAAACTACGAGATCGCCCTCAAGAACACCCTGCGCCAGGCCCCCGACGTGATCCTCATCGGCGAGATCCGCG
ACCGCGAAACCATGGATTACGCCATTGCCTTCGCCGAGACCGGTCACCTGTGCCTGAGCACCCTGCATGCCAACAGCACC
AACCAGGCCCTGGACCGCATCATCAACTTCTTCCCCGAGGAACGCCGCCAGCAGCTGCTGATGGATTTGTCGTTGAACCT
GAAGGCGGTGGTGTCCCAGCGTCTGATCCCGCTGGCCACCGGCAAGGGGCGGGTGCCGGCGGTGGAGATCATGATCAACA
GCCCGCTGATGTCGGATCTGATCTTCAAGGGCGAGATCCACGAGATGAAGGCGCTGATGGCCAAGTCCAACGAGGCGGGC
ATGCAAACCTTCGACCAGGCCCTGTTCCAGCTCTTCGAGACCGGCAGGATCACCTACGAGGATGCCCTGCGCAACGCCGA
CTCCGTCAACGACCTGCGTCTGCGCATCAAGCTGGACAGCAAGCGCGCCCGCAGCCAGAACCTGCTGGACGACACCGAAG
GTCTGGAGATGGAGGCCACCCCGGAGGAGTCCGGGCTGCTCAAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(54-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.93

96.134

0.595
  pilU Acinetobacter baylyi ADP1

58.537

95.103

0.557
  pilU Vibrio cholerae strain A1552

56.034

89.691

0.503
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.179

86.34

0.381
  pilT Legionella pneumophila strain ERS1305867

44.109

85.309

0.376
  pilT Legionella pneumophila strain Lp02

44.109

85.309

0.376
  pilT Pseudomonas aeruginosa PAK

43.284

86.34

0.374
  pilT Acinetobacter baylyi ADP1

42.73

86.856

0.371
  pilT Acinetobacter baumannii D1279779

43.202

85.309

0.369
  pilT Acinetobacter baumannii strain A118

43.202

85.309

0.369
  pilT Acinetobacter nosocomialis M2

43.202

85.309

0.369
  pilT Pseudomonas stutzeri DSM 10701

42.388

86.34

0.366

Multiple sequence alignment