Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACLD71_RS00680 Genome accession   NZ_CP177241
Coordinates   143638..144672 (-) Length   344 a.a.
NCBI ID   WP_137674185.1    Uniprot ID   -
Organism   Agarivorans sp. DSG3-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 138638..149672
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLD71_RS00665 (ACLD71_00665) - 140302..141042 (-) 741 WP_303512341.1 OmpA family protein -
  ACLD71_RS00670 (ACLD71_00670) yaaA 141713..142492 (-) 780 WP_411992400.1 peroxide stress protein YaaA -
  ACLD71_RS00675 (ACLD71_00675) pilU 142511..143629 (-) 1119 WP_411992402.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACLD71_RS00680 (ACLD71_00680) pilT 143638..144672 (-) 1035 WP_137674185.1 type IV pilus twitching motility protein PilT Machinery gene
  ACLD71_RS00685 (ACLD71_00685) - 144698..145396 (+) 699 WP_411992404.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACLD71_RS00690 (ACLD71_00690) proC 145421..146242 (+) 822 WP_411992406.1 pyrroline-5-carboxylate reductase -
  ACLD71_RS00695 (ACLD71_00695) - 146260..146835 (+) 576 WP_137674188.1 YggT family protein -
  ACLD71_RS00700 (ACLD71_00700) yggU 146836..147123 (+) 288 WP_137674189.1 DUF167 family protein YggU -
  ACLD71_RS00705 (ACLD71_00705) - 147182..147616 (+) 435 WP_137674190.1 DUF4426 domain-containing protein -
  ACLD71_RS00710 (ACLD71_00710) rdgB 147718..148317 (+) 600 WP_137674191.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  ACLD71_RS00715 (ACLD71_00715) hemW 148311..149459 (+) 1149 WP_411992408.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38368.97 Da        Isoelectric Point: 6.3897

>NTDB_id=1086361 ACLD71_RS00680 WP_137674185.1 143638..144672(-) (pilT) [Agarivorans sp. DSG3-1]
MDVTELLAFSVKHNASDLHLSAELPPMIRVDGEVRKINMPALEHKQVHSLIYDIMNDKQRKEYEENLEIDFSFEVPSVAR
FRVNAFQQSRGAAAVFRTIPSEVLNLDQLGAPAIFRQIADHPRGLVLVTGPTGSGKSTTLAAMIDYVNEQRHEHILTIED
PIEFVHKNKNCLINQREVHRDTHSFNNALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAAEKDMVRSMLSESLRAVIAQTLLKKLGGGRVAAHEIMIGIPAIRNLIREDKVAQMYSVIQTGMAHGMQTLDQNL
KELVNQGMVSYEDAKLKAADANNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1086361 ACLD71_RS00680 WP_137674185.1 143638..144672(-) (pilT) [Agarivorans sp. DSG3-1]
ATGGATGTTACTGAATTACTGGCCTTTAGTGTAAAACATAACGCGTCGGATCTACACCTATCTGCCGAATTACCACCAAT
GATTCGAGTTGATGGCGAAGTGCGTAAAATTAATATGCCAGCATTAGAGCATAAACAAGTACACAGCTTGATTTATGACA
TTATGAACGACAAGCAGCGCAAAGAGTACGAAGAAAACTTAGAGATCGATTTCTCGTTTGAAGTGCCAAGTGTTGCTCGC
TTCAGGGTAAATGCTTTTCAACAAAGCCGCGGCGCAGCAGCAGTGTTTCGTACCATTCCCTCGGAAGTATTGAATTTAGA
CCAATTGGGCGCGCCTGCTATCTTTCGCCAAATTGCAGATCACCCACGTGGCTTAGTTTTAGTCACCGGCCCTACTGGTT
CGGGTAAATCAACGACCTTGGCGGCGATGATTGATTATGTGAATGAGCAACGTCACGAGCATATTCTGACGATTGAAGAC
CCTATCGAATTTGTACACAAAAACAAAAACTGTTTGATTAACCAGCGTGAAGTTCACCGTGATACGCACAGCTTTAACAA
CGCCTTGCGTTCGGCGCTGCGTGAAGATCCCGATATTATTCTGGTGGGTGAATTACGTGATTTGGAAACCATTCGCTTAG
CGCTTACCGCGGCCGAAACCGGTCACTTAGTATTTGGCACCTTGCACACTACCTCGGCGGCTAAAACCATTGACCGTGTG
ATTGATGTATTCCCAGCGGCTGAAAAAGACATGGTTCGTTCAATGTTGTCTGAATCATTACGTGCAGTAATTGCGCAAAC
CCTGCTTAAAAAACTAGGTGGCGGCCGTGTTGCAGCCCATGAGATTATGATTGGTATACCAGCCATTCGTAACTTGATTC
GAGAAGATAAAGTGGCGCAAATGTACTCGGTAATTCAAACCGGTATGGCGCATGGCATGCAAACCCTTGATCAAAACCTT
AAAGAGTTAGTGAACCAAGGCATGGTGTCATATGAAGACGCTAAGCTTAAAGCAGCTGACGCAAATAACTTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.651

100

0.797
  pilT Acinetobacter baumannii D1279779

79.651

100

0.797
  pilT Acinetobacter baumannii strain A118

79.651

100

0.797
  pilT Pseudomonas stutzeri DSM 10701

78.198

100

0.782
  pilT Pseudomonas aeruginosa PAK

77.907

100

0.779
  pilT Acinetobacter baylyi ADP1

77.616

100

0.776
  pilT Legionella pneumophila strain Lp02

74.128

100

0.741
  pilT Legionella pneumophila strain ERS1305867

74.128

100

0.741
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

74.777

97.965

0.733
  pilT Vibrio cholerae strain A1552

74.777

97.965

0.733
  pilT Neisseria meningitidis 8013

67.251

99.419

0.669
  pilT Neisseria gonorrhoeae MS11

66.959

99.419

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

100

0.5
  pilU Vibrio cholerae strain A1552

41.279

100

0.413
  pilU Pseudomonas stutzeri DSM 10701

40.87

100

0.41
  pilU Acinetobacter baylyi ADP1

40.87

100

0.41

Multiple sequence alignment