Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACLD71_RS00675 Genome accession   NZ_CP177241
Coordinates   142511..143629 (-) Length   372 a.a.
NCBI ID   WP_411992402.1    Uniprot ID   -
Organism   Agarivorans sp. DSG3-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 137511..148629
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLD71_RS00655 (ACLD71_00655) srmB 137685..138941 (+) 1257 WP_137674859.1 ATP-dependent RNA helicase SrmB -
  ACLD71_RS00665 (ACLD71_00665) - 140302..141042 (-) 741 WP_303512341.1 OmpA family protein -
  ACLD71_RS00670 (ACLD71_00670) yaaA 141713..142492 (-) 780 WP_411992400.1 peroxide stress protein YaaA -
  ACLD71_RS00675 (ACLD71_00675) pilU 142511..143629 (-) 1119 WP_411992402.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACLD71_RS00680 (ACLD71_00680) pilT 143638..144672 (-) 1035 WP_137674185.1 type IV pilus twitching motility protein PilT Machinery gene
  ACLD71_RS00685 (ACLD71_00685) - 144698..145396 (+) 699 WP_411992404.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACLD71_RS00690 (ACLD71_00690) proC 145421..146242 (+) 822 WP_411992406.1 pyrroline-5-carboxylate reductase -
  ACLD71_RS00695 (ACLD71_00695) - 146260..146835 (+) 576 WP_137674188.1 YggT family protein -
  ACLD71_RS00700 (ACLD71_00700) yggU 146836..147123 (+) 288 WP_137674189.1 DUF167 family protein YggU -
  ACLD71_RS00705 (ACLD71_00705) - 147182..147616 (+) 435 WP_137674190.1 DUF4426 domain-containing protein -
  ACLD71_RS00710 (ACLD71_00710) rdgB 147718..148317 (+) 600 WP_137674191.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41548.66 Da        Isoelectric Point: 6.0936

>NTDB_id=1086360 ACLD71_RS00675 WP_411992402.1 142511..143629(-) (pilU) [Agarivorans sp. DSG3-1]
MLIDELLSDMSERKASDLYLSVGVPPSAKVSGRLTPLSEHKLMPEDVMEMLATIRTPAQIEAFESGREANFAIARKGLGR
YRVSAFFQREQPSMVIRRIETDIPSFEQLQLPQILKEVGMSKRGLILFVGATGAGKSTTQASMIDYRNHNSSGHILTIED
PVEFMHKHAGCIVNQREVGIDTPSFEEALKNSLRQAPDVILIGEIRTRETMEFALQFSETGHLCMATLHANNANQALDRI
MHLVPEERHRQLCFDLSFNLRAIVAQQLVPTKDGNGRRGVFEILLNTPLAADLIRKGDMHKLKELMTKSREQGMLTFDQS
LFELYESGVIGYAESIAHADSPNDLRLMIKLHGSEKSNNLDGGMLDGVTIDF

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=1086360 ACLD71_RS00675 WP_411992402.1 142511..143629(-) (pilU) [Agarivorans sp. DSG3-1]
ATGTTAATTGATGAACTACTGAGTGATATGTCGGAGCGTAAAGCCTCCGATTTATACTTATCGGTTGGCGTTCCTCCTAG
TGCAAAAGTGAGTGGTCGCTTAACACCGTTAAGCGAGCATAAGCTCATGCCTGAAGATGTTATGGAAATGCTGGCAACCA
TTCGCACGCCCGCTCAAATTGAGGCATTTGAAAGCGGACGAGAGGCTAACTTTGCTATCGCCCGTAAAGGTTTGGGCCGT
TATCGTGTGAGCGCATTTTTTCAGCGCGAACAGCCAAGCATGGTGATTCGTAGAATTGAAACCGATATTCCCAGCTTTGA
GCAGCTTCAACTGCCACAAATTCTTAAAGAAGTGGGAATGTCTAAACGTGGTTTGATTCTGTTTGTAGGGGCAACAGGTG
CCGGTAAATCGACTACTCAGGCGAGCATGATTGATTACCGCAACCACAATTCTAGTGGGCATATTCTTACCATTGAAGAC
CCAGTGGAATTTATGCACAAACATGCTGGTTGTATTGTTAATCAGCGAGAAGTGGGCATTGATACGCCGTCGTTTGAAGA
AGCGTTGAAAAACTCGTTGCGCCAAGCGCCAGACGTTATTCTTATTGGTGAGATCCGTACTCGTGAAACCATGGAATTTG
CCCTGCAGTTCTCTGAAACCGGCCATTTATGTATGGCGACCTTGCACGCCAACAACGCCAACCAAGCCTTAGACCGAATT
ATGCACCTTGTGCCTGAAGAACGTCATCGCCAGTTATGTTTTGATTTGTCTTTTAACTTGCGAGCGATTGTGGCGCAGCA
GTTAGTGCCAACCAAAGATGGTAATGGGCGCCGTGGTGTATTTGAGATCCTACTTAACACCCCATTGGCTGCAGATTTAA
TTCGTAAAGGCGACATGCATAAGCTGAAAGAGCTCATGACTAAGTCGCGTGAACAGGGCATGTTAACCTTTGACCAAAGC
TTGTTTGAACTCTATGAATCGGGTGTGATTGGCTATGCCGAGTCAATTGCGCACGCAGATTCGCCAAACGATTTGCGCTT
GATGATTAAATTGCATGGCTCTGAAAAATCGAATAACTTAGATGGCGGAATGTTAGATGGGGTAACCATCGATTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.408

95.43

0.586
  pilU Acinetobacter baylyi ADP1

59.053

96.505

0.57
  pilU Vibrio cholerae strain A1552

56.183

100

0.562
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.691

92.204

0.384
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.719

89.785

0.366
  pilT Vibrio cholerae strain A1552

40.719

89.785

0.366
  pilT Legionella pneumophila strain Lp02

40.785

88.978

0.363
  pilT Legionella pneumophila strain ERS1305867

40.785

88.978

0.363

Multiple sequence alignment