Detailed information NTG1086154

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: ACK8NJ_RS04160
• Genome accession: NZ_CP177202
• Coordinates: 871152..872132 (+)
• Length: 326 a.a.
• NCBI ID: WP_071794655.1
• Uniprot ID: A0A4Y8GK02
• Organism: Escherichia sp. F1
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 866152..877132

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACK8NJ_RS04130 (ACK8NJ_04130)	-	866528..867514 (-)	987	WP_000784004.1	TRAP transporter substrate-binding protein	-
ACK8NJ_RS04135 (ACK8NJ_04135)	hemW	867829..868965 (-)	1137	WP_000239965.1	radical SAM family heme chaperone HemW	-
ACK8NJ_RS04140 (ACK8NJ_04140)	-	868958..869551 (-)	594	WP_001174747.1	XTP/dITP diphosphatase	-
ACK8NJ_RS04145 (ACK8NJ_04145)	yggU	869559..869849 (-)	291	WP_001277223.1	DUF167 family protein YggU	-
ACK8NJ_RS04150 (ACK8NJ_04150)	yggT	869846..870412 (-)	567	WP_097336252.1	osmotic shock tolerance protein YggT	-
ACK8NJ_RS04155 (ACK8NJ_04155)	yggS	870430..871134 (-)	705	WP_001521256.1	pyridoxal phosphate homeostasis protein	-
ACK8NJ_RS04160 (ACK8NJ_04160)	pilT	871152..872132 (+)	981	WP_071794655.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACK8NJ_RS04165 (ACK8NJ_04165)	ruvX	872247..872663 (-)	417	WP_000017106.1	Holliday junction resolvase RuvX	-
ACK8NJ_RS04170 (ACK8NJ_04170)	-	872663..873226 (-)	564	WP_001355636.1	YqgE/AlgH family protein	-
ACK8NJ_RS04175 (ACK8NJ_04175)	gshB	873335..874285 (-)	951	WP_000593273.1	glutathione synthase	-
ACK8NJ_RS04180 (ACK8NJ_04180)	rsmE	874298..875029 (-)	732	WP_001222509.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
ACK8NJ_RS04185 (ACK8NJ_04185)	endA	875109..875816 (-)	708	WP_000286500.1	deoxyribonuclease I	-
ACK8NJ_RS04190 (ACK8NJ_04190)	-	875911..876408 (-)	498	WP_001296359.1	SprT family zinc-dependent metalloprotease	-

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 36030.18 Da
• Isoelectric Point: 5.5925

>NTDB_id=1086154 ACK8NJ_RS04160 WP_071794655.1 871152..872132(+) (pilT) [Escherichia sp. F1]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRTILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLVNTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VEEGRL

Nucleotide

• Length: 981 bp

>NTDB_id=1086154 ACK8NJ_RS04160 WP_071794655.1 871152..872132(+) (pilT) [Escherichia sp. F1]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGCAGAATGGAAGCTGCGCCGTTTGACGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGACAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCCGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCGCTGACGG
CAGCAGAAACCGGACACCTGGTGCTGGCAACTTTACATACGCGTGGTGCGGCGCAGGCAGTTGAGCGGTTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCGGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTGCTGGTTAACACACCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGAGGAAGGACGTTTGTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (62-239)

3D structure

• AlphaFold DB: A0A4Y8GK02

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae strain A1552	49.541	100	0.497
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	49.541	100	0.497
pilT	Neisseria meningitidis 8013	48.476	100	0.488
pilT	Neisseria gonorrhoeae MS11	48.171	100	0.485
pilT	Acinetobacter baylyi ADP1	46.483	100	0.466
pilT	Acinetobacter baumannii D1279779	46.177	100	0.463
pilT	Acinetobacter nosocomialis M2	46.177	100	0.463
pilT	Acinetobacter baumannii strain A118	46.177	100	0.463
pilT	Pseudomonas stutzeri DSM 10701	46.177	100	0.463
pilT	Pseudomonas aeruginosa PAK	45.872	100	0.46
pilT	Legionella pneumophila strain ERS1305867	45.26	100	0.454
pilT	Legionella pneumophila strain Lp02	45.26	100	0.454
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.138	97.546	0.411
pilU	Vibrio cholerae strain A1552	40.379	97.239	0.393
pilU	Pseudomonas stutzeri DSM 10701	37.273	100	0.377
pilU	Acinetobacter baylyi ADP1	36.646	98.773	0.362
pilB	Legionella pneumophila strain ERS1305867	30.89	100	0.362