Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACLB90_RS14580 Genome accession   NZ_CP177163
Coordinates   3345995..3347032 (+) Length   345 a.a.
NCBI ID   WP_019185385.1    Uniprot ID   A0A498C434
Organism   Stenotrophomonas sp. LGBM10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3340995..3352032
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLB90_RS14555 (ACLB90_14555) soxR 3341924..3342358 (+) 435 WP_411850126.1 redox-sensitive transcriptional activator SoxR -
  ACLB90_RS14560 (ACLB90_14560) - 3342404..3343006 (-) 603 WP_411850127.1 short chain dehydrogenase -
  ACLB90_RS14565 (ACLB90_14565) - 3343101..3343994 (+) 894 WP_411850128.1 LysR substrate-binding domain-containing protein -
  ACLB90_RS14570 (ACLB90_14570) proC 3344001..3344822 (-) 822 WP_411850129.1 pyrroline-5-carboxylate reductase -
  ACLB90_RS14575 (ACLB90_14575) - 3345237..3345908 (-) 672 WP_411850130.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACLB90_RS14580 (ACLB90_14580) pilT 3345995..3347032 (+) 1038 WP_019185385.1 type IV pilus twitching motility protein PilT Machinery gene
  ACLB90_RS14585 (ACLB90_14585) pilU 3347118..3348254 (+) 1137 WP_411850131.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACLB90_RS14590 (ACLB90_14590) - 3348371..3349051 (+) 681 WP_411850132.1 RDD family protein -
  ACLB90_RS14595 (ACLB90_14595) - 3349116..3349937 (-) 822 WP_411850133.1 alpha/beta fold hydrolase -
  ACLB90_RS14600 (ACLB90_14600) - 3350117..3350332 (+) 216 WP_411850134.1 DUF4287 domain-containing protein -
  ACLB90_RS14605 (ACLB90_14605) - 3350420..3351130 (+) 711 WP_411850135.1 YitT family protein -
  ACLB90_RS14610 (ACLB90_14610) - 3351115..3351648 (-) 534 WP_411850136.1 DNA-3-methyladenine glycosylase I -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38601.38 Da        Isoelectric Point: 6.5779

>NTDB_id=1085714 ACLB90_RS14580 WP_019185385.1 3345995..3347032(+) (pilT) [Stenotrophomonas sp. LGBM10]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLACPPIFRELIDQPQGLILVTGPTGSGKSTTLAAMIDHINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQQMGMMTLDQHL
QDLVKRSLITRNQAREYAKDKRIFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1085714 ACLB90_RS14580 WP_019185385.1 3345995..3347032(+) (pilT) [Stenotrophomonas sp. LGBM10]
ATGGATATCGCCGAACTGTTGGCGTTTTCCGTAAAGAACAAAGCGTCGGATCTGCATCTGTCGGCCGGCTTGCCGCCGAT
GATCCGCGTGGACGGCGATGTCCGTCGCATCAACATTCCGGCCCTGGACCACAAGCAGGTCCACGCGCTGGTGTACGACA
TCATGTCGGACAAGCAGCGCCGCGATTACGAGGAGTTCCTCGAAGTCGACTTCTCGTTCGAGATCCCGTCGCTGGCCCGG
TTCCGTGTCAACGCGTTCAACCAGAACCGCGGCGCCGGTGCGGTGTTCCGTACCATTCCGTCGGAGGTGCTCACGCTGGA
AGACCTGGCGTGCCCGCCGATCTTCCGTGAGCTGATCGACCAGCCGCAGGGCCTGATCCTGGTCACCGGTCCGACCGGTT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCGACCACATCAACAAGAACGAATACGGCCACATCCTCACCGTCGAGGAC
CCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTGCACCGTGATACGCACGGCTTCAACGA
AGCGCTGCGTTCGGCGCTGCGTGAAGATCCGGACATCATCCTGGTCGGCGAACTGCGTGACCTGGAAACCATCCGCCTGG
CGTTGACCGCCGCGGAAACCGGCCACCTGGTGTTCGGCACGCTGCACACCAGTTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCCGCCGGCGAGAAGCCGATGGTGCGCTCGATGCTGTCCGAGTCGCTGCGTGCGGTGATCTCGCAGGC
GCTGTTGAAGAAGGTGGGCGGCGGTCGTACCGCCGCATGGGAAATCATGGTGGGCACCCCGGCCATCCGCAACCTGATCC
GTGAGGACAAGGTGGCGCAGATGTACTCGGCGATCCAGACGGGCCAGCAGATGGGCATGATGACGCTCGACCAGCACCTC
CAGGACCTGGTCAAGCGCAGCCTGATCACGCGCAACCAGGCCAGGGAATACGCCAAGGACAAGCGCATCTTCGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A498C434

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.942

99.71

0.797
  pilT Acinetobacter baumannii D1279779

79.942

99.71

0.797
  pilT Acinetobacter baumannii strain A118

79.942

99.71

0.797
  pilT Pseudomonas stutzeri DSM 10701

78.488

99.71

0.783
  pilT Acinetobacter baylyi ADP1

78.198

99.71

0.78
  pilT Pseudomonas aeruginosa PAK

78.761

98.261

0.774
  pilT Legionella pneumophila strain Lp02

74.709

99.71

0.745
  pilT Legionella pneumophila strain ERS1305867

74.709

99.71

0.745
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.821

95.362

0.675
  pilT Vibrio cholerae strain A1552

70.821

95.362

0.675
  pilT Neisseria meningitidis 8013

65.689

98.841

0.649
  pilT Neisseria gonorrhoeae MS11

65.396

98.841

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

100

0.499
  pilU Acinetobacter baylyi ADP1

40.588

98.551

0.4
  pilU Pseudomonas stutzeri DSM 10701

40.413

98.261

0.397
  pilU Vibrio cholerae strain A1552

40.299

97.101

0.391

Multiple sequence alignment