Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACLB90_RS14585 Genome accession   NZ_CP177163
Coordinates   3347118..3348254 (+) Length   378 a.a.
NCBI ID   WP_411850131.1    Uniprot ID   -
Organism   Stenotrophomonas sp. LGBM10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3342118..3353254
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLB90_RS14560 (ACLB90_14560) - 3342404..3343006 (-) 603 WP_411850127.1 short chain dehydrogenase -
  ACLB90_RS14565 (ACLB90_14565) - 3343101..3343994 (+) 894 WP_411850128.1 LysR substrate-binding domain-containing protein -
  ACLB90_RS14570 (ACLB90_14570) proC 3344001..3344822 (-) 822 WP_411850129.1 pyrroline-5-carboxylate reductase -
  ACLB90_RS14575 (ACLB90_14575) - 3345237..3345908 (-) 672 WP_411850130.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACLB90_RS14580 (ACLB90_14580) pilT 3345995..3347032 (+) 1038 WP_019185385.1 type IV pilus twitching motility protein PilT Machinery gene
  ACLB90_RS14585 (ACLB90_14585) pilU 3347118..3348254 (+) 1137 WP_411850131.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACLB90_RS14590 (ACLB90_14590) - 3348371..3349051 (+) 681 WP_411850132.1 RDD family protein -
  ACLB90_RS14595 (ACLB90_14595) - 3349116..3349937 (-) 822 WP_411850133.1 alpha/beta fold hydrolase -
  ACLB90_RS14600 (ACLB90_14600) - 3350117..3350332 (+) 216 WP_411850134.1 DUF4287 domain-containing protein -
  ACLB90_RS14605 (ACLB90_14605) - 3350420..3351130 (+) 711 WP_411850135.1 YitT family protein -
  ACLB90_RS14610 (ACLB90_14610) - 3351115..3351648 (-) 534 WP_411850136.1 DNA-3-methyladenine glycosylase I -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42195.55 Da        Isoelectric Point: 6.8286

>NTDB_id=1085715 ACLB90_RS14585 WP_411850131.1 3347118..3348254(+) (pilU) [Stenotrophomonas sp. LGBM10]
MSTTTIDFTSFLKLMAHQKASDLFITAGMPPAIKVNGKISPITQTPLTPQQSRDLVLNVMTPAQREEFEKTHECNFAIGL
SGVGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIGYRNHNSTGHI
ITIEDPIEFVHKHEGCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQ
AMDRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPSPDGKSRKVAMEIMLGTPLVQDYIRDGEIHKLKEVMKESVQLGMR
TFDQSLYELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDARTLSQGLDGVEISEIR

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=1085715 ACLB90_RS14585 WP_411850131.1 3347118..3348254(+) (pilU) [Stenotrophomonas sp. LGBM10]
ATGAGCACCACCACCATCGACTTCACCTCGTTCCTCAAGCTGATGGCGCACCAGAAGGCGTCAGACCTGTTCATCACTGC
GGGCATGCCGCCGGCGATCAAGGTCAACGGCAAGATCTCGCCCATCACCCAGACCCCGCTGACGCCGCAGCAGAGCCGCG
ACCTGGTGCTGAACGTGATGACGCCGGCACAGCGCGAAGAGTTCGAGAAAACCCACGAATGCAACTTCGCCATCGGCCTG
TCCGGTGTCGGCCGTTTCCGCGTGAGCTGCTTCTACCAGCGCAACCAGGTTGGCATGGTGCTGCGTCGCATCGAGACGCG
CATTCCCACCGTGGAAGAGCTGAGCCTGCCGCCGATCATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCCTGTTCG
TGGGCGCGACCGGTACCGGCAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCACAACTCCACCGGGCACATC
ATCACCATCGAAGACCCGATCGAGTTCGTGCACAAGCATGAAGGCTGCATCATCACCCAGCGCGAAGTCGGCATCGATAC
CGACAGCTGGGAAGCGGCGCTGAAGAACACGCTGCGCCAGGCACCGGACGTCATCATGATCGGCGAAGTGCGTACCCGCG
AAGGCATGGACCACGCCATCGCGTTTGCCGAGACCGGCCACCTGGTGCTGTGTACGCTGCATGCCAACAACGCCAACCAG
GCGATGGACCGCATCATCAACTTCTTCCCCGAGGACCGCCGCAACCAGCTGCTGATGGATCTGTCGCTGAACCTCAAGGG
CGTGGTTGCCCAGCAGCTGATTCCCTCGCCGGATGGCAAGTCGCGCAAGGTGGCGATGGAGATCATGCTGGGCACGCCGC
TGGTGCAGGACTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAAGTGATGAAGGAATCGGTGCAGTTGGGCATGCGC
ACCTTCGACCAGAGTCTGTACGAGCTGTACCAGGCCGGTGAGATCAGCTACGAGGACGCCCTGCGCTACGCCGACTCGCA
GAACGAGGTGCGCCTGCGCATCAAGCTGTCGCAGGGCGGTGATGCGCGCACGCTGTCGCAGGGCCTGGATGGGGTGGAGA
TCTCCGAAATCCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.847

97.09

0.659
  pilU Acinetobacter baylyi ADP1

63.585

94.444

0.601
  pilU Vibrio cholerae strain A1552

53.652

94.18

0.505
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.458

94.709

0.402
  pilT Acinetobacter baumannii strain A118

40.407

91.005

0.368
  pilT Acinetobacter nosocomialis M2

40.407

91.005

0.368
  pilT Acinetobacter baumannii D1279779

40.407

91.005

0.368
  pilT Pseudomonas aeruginosa PAK

40.896

88.624

0.362
  pilT Legionella pneumophila strain Lp02

40.896

88.624

0.362
  pilT Legionella pneumophila strain ERS1305867

40.896

88.624

0.362

Multiple sequence alignment